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Nucleic acids or proteins in a complex sample can be visualized using electrophoresis and blotting procedures and the compatible reagents. Improving the overall efficiency of electrophoresis systems, these reagents accommodate a variety of blotting techniques and with antibodies provided against tens of thousands of different proteins. These methods are used in the fields of molecular biology, immunogenetics, and other molecular biology disciplines.

Nucleic acids or proteins in a complex sample can be visualized using electrophoresis and blotting procedures and the compatible reagents. Improving the overall efficiency of electrophoresis systems, these reagents accommodate a variety of blotting techniques and with antibodies provided against tens of thousands of different proteins. These methods are used in the fields of molecular biology, immunogenetics, and other molecular biology disciplines.


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Description: The Fast Western Blot Kits, SuperSignal™ West Dura provide reagents and protocol for reliable and rapid, 1 hour chemiluminescent Western blotting at femtogram levels of sensitivity. These complete Fast Western Blot Kits requires minimal hands on time and yield results comparable to classical Western blotting with SuperSignal™ West Dura Chemiluminescent Substrate. This substrate produces a signal that is detectable using photographic or other imaging methods. The developed blots can be repeatedly exposed and stripped of the primary antibody and reprobed. The complete kit contains all the reagents necessary to block and probe a Western blot with mouse or rabbit primary antibody.
Catalog Number: PIER35071
UOM: 1 * 1 KIT
Supplier: Thermo Fisher Scientific


Description: QuantaRed™ Enhanced Chemifluorescent HRP Substrate contains chemical enhancers to increase the fluorescent yield and sensitivity of 10-acetyl-3,7-dihydroxyphenoxazine (ADHP) chemifluorescence.
Catalog Number: PIER15159
UOM: 1 * 1 items
Supplier: Thermo Fisher Scientific


Description: For the hybridization of probes to nucleic acids in Northern and Southern blotting.
Catalog Number: J63618.AD
UOM: 1 * 50 mL
Supplier: Thermo Fisher Scientific

Description: The appearance of streaks that distort 2-D electrophoresis maps is a common problem, occurring most frequently when running gels that contain regions greater than pH 7,0. Increased sample load, increased length of the IPG strip, or using a narrower pH gradient worsens the problem. Extra spots on 2-D gels, caused by non specific oxidation of proteins, is another difficulty encountered when running gels containing basic regions. Both streaking and non specific oxidation result in poorly resolved protein patterns and reduced reproducibility between electrophoresis runs.
Catalog Number: 17-6003-18
UOM: 1 * 1 mL
Supplier: Cytiva


Description: AlkPhos Direct™ Labelling and detection systems are based on the rapid, direct labelling of DNA or RNA probes with thermostable alkaline phosphatase. AlkPhos Direct™ combines the convenience of direct enzyme labelling (no blocking or antibody stages) with those of alkaline phosphatase detection (long light output and high sensitivity).
Catalog Number: RPN3682
UOM: 1 * 1 items
Supplier: Cytiva


Description: ECL Direct Nucleic Acid Labeling and Detection Systems are based on the direct labeling of DNA or RNA probes with horseradish peroxidase (HRP) in a simple 20 min chemical reaction. The resulting probe can be used without purification. Detection is achieved by generation of light via the HRP-catalyzed breakdown of luminol.
Catalog Number: RPN3006
UOM: 1 * 1 items
Supplier: Cytiva


Description: ECF™ substrate for Western blotting
Catalog Number: RPN5785
UOM: 1 * 1 items
Supplier: Cytiva


Description: ECL Direct Nucleic Acid Labeling and Detection Systems are based on the direct labeling of DNA or RNA probes with horseradish peroxidase (HRP) in a simple 20 minute chemical reaction. The resulting probe can be used without purification. Detection is achieved by generation of light via the HRP-catalyzed breakdown of luminol.
Catalog Number: RPN3005
UOM: 1 * 1 items
Supplier: Cytiva


Description: Enhanced chemiluminescence (ECL) detection reagents for reliable detection of medium and high expression proteins.
Catalog Number: RPN2109
UOM: 1 * 1 KIT
Supplier: Cytiva

Description: Cy™ fluorescent amidites for incorporation of Cy3, Cy3,5, Cy5, Cy5,5 fluorescent dyes at any position in an oligonucleotide during synthesis.
Catalog Number: 28-9042-49
UOM: 1 * 100 mg
Supplier: Cytiva


Description: Chemiluminescence detection reagent for detection of high to medium abundant proteins.
Catalog Number: RPN3243
UOM: 1 * 1 KIT
Supplier: Cytiva


Description: Amersham ECL Select is a high sensitivity reagent for chemiluminescent Western blotting detection.
Catalog Number: RPN2235
UOM: 1 * 1 KIT
Supplier: Cytiva

Description: CypHer5E is a red-excitable, pH-sensitive cyanine dye derivative that is minimally fluorescent at a basic pH and maximally fluorescent at an acidic pH.
Catalog Number: PA15401
UOM: 1 * 1 mg
Supplier: Cytiva


Description: Amersham™ ECL™ Prime is a highly sensitive Western blotting detection reagent characterised by stable signal emission, allowing for the possibility of repeated exposures and making it easier to process several blots in the same experimental run. Amersham™ ECL™ Prime delivers a detection system that is sensitive, stable, precisely quantitative across a wide dynamic range of protein levels, and conservative in its consumption of expensive antibody reagents. The high performance of Amersham™ ECL™ Prime is due to the presence of an enhancer in the reagent, increasing enzyme turnover, and thereby significantly increasing both signal intensity and duration. The light signal is further intensified by the addition of a catalyst.
Catalog Number: RPN2232SK
UOM: 1 * 1 KIT
Supplier: Cytiva


Description: Pharmalyte® carrier ampholytes, prepared by the co-polymerization of glycine, glycylglycine, amines and epichlorohydrin, are available in five broad-range and four narrow-range pH intervals.
Catalog Number: 17-0452-01
UOM: 1 * 25 mL
Supplier: Cytiva

Description: Amersham ECL Blocking Agent is for blocking nitrocellulose and PVDF membranes to be used with Amersham ECL, Amersham ECL Prime and Amersham ECL Select Western Blotting Detection Reagents
Catalog Number: RPN2125
UOM: 1 * 40 g
Supplier: Cytiva


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