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You Searched For: Western Blotting Reagents

Nucleic acids or proteins in a complex sample can be visualized using electrophoresis and blotting procedures and the compatible reagents. Improving the overall efficiency of electrophoresis systems, these reagents accommodate a variety of blotting techniques and with antibodies provided against tens of thousands of different proteins. These methods are used in the fields of molecular biology, immunogenetics, and other molecular biology disciplines.

Nucleic acids or proteins in a complex sample can be visualized using electrophoresis and blotting procedures and the compatible reagents. Improving the overall efficiency of electrophoresis systems, these reagents accommodate a variety of blotting techniques and with antibodies provided against tens of thousands of different proteins. These methods are used in the fields of molecular biology, immunogenetics, and other molecular biology disciplines.


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Product Image-PIER20158

Chemiluminescent RNA EMSA kit, LightShift™

Catalog Number: (PIER20158)
Supplier: Thermo Fisher Scientific
Description: LightShift™ Chemiluminescent RNA EMSA Kit provides a non-radioactive solution for studying RNA-protein interactions using an electrophoretic mobility-shift assay (EMSA).
UOM: 1 * 1 items
Product Image-PIER80196X3

Western Blotting Substrate, Pierce™ ECL Plus

Supplier: Thermo Fisher Scientific
Description: The Pierce™ ECL Plus Western Blotting Substrate is an acridan-based chemiluminescent and chemifluorescent HRP substrate for Western blot detection using X-ray film or CCD- or laser-based imagers.

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Product Image-786-123T

Sample clean up, PAGE-Perfect™

Supplier: G-Biosciences
Description: Many lysis buffers and reagents used in sample preparation are incompatible with routinely used electrophoretic analysis. The presence of contaminants, or interfering agents, such as salts, acids, bases and detergents, result in band distortion and poor protein resolution. As a result, SDS-PAGE gels are hard to analyse and lack reproducibility. PAGE-Perfect™ is a simple, two-step method for concentrating, cleaning and preparing protein solutions for running publication quality gels.

Image Unavailable-69078-3

Western blotting kit, Perfect Protein™, Novagen®

Catalog Number: (69078-3)
Supplier: Merck Millipore (Novagen)
Description: The Perfect Protein™ HRP Western Blot Kit includes an HRP or AP conjugate in addition to Perfect Protein™ Western markers.
UOM: 1 * 1 items
Image Unavailable-J60561.AK

SSC (20X), RNAse free

Supplier: Thermo Fisher Scientific
Description: SSC solution is commonly used in nucleic acid hybridization techniques at concentrations ranging from 0,1X to 20X depending on the application. It is used to facilitate transfer of nucleic acids to membranes in Southern and Northern blotting, dot blotting, and colony and plaque lifts.

MSDS Certificates

Product Image-ROCKFEMTOMAX-020

Chemiluminescent super sensitive HRP substrate, FemtoMax™

Supplier: Rockland Immunochemicals
Description: FemtoMax™ is an extremely sensitive, non radioactive, and enhanced luminol-based chemiluminescent substrate for the detection of horseradish peroxidase (HRP). It allows for the detection of femtogram (10 to 15) amounts of antigen using photographic film or other imaging methods including highly sensitive CCD cameras.

Product Image-PIER35065

Fast western blotting kit, SuperSignal™ west Pico, Pierce™

Supplier: Thermo Fisher Scientific
Description: The Fast Western Blot Kit, SuperSignal™ West Pico Substrate contains optimised reagents and protocol for picogram-level detection and and Western blot development less than 1 hour after gel transfer.

Product Image-ROCK18-8815-33

Western blot kits, TrueBlot®

Supplier: Rockland Immunochemicals
Description: TrueBlot® Western blot kits contain the critical supporting reagents, buffers, and substrates for immunoprecipitation and Western blotting of samples using TrueBlot® second step immunoblotting reagents in conjunction with the user's own primary IP antibody and primary (mouse, goat or rabbit IgG) Western blotting antibody.

Image Unavailable-WB59-100

Western-Re-Probe™ reagent, Calbiochem®

Catalog Number: (WB59-100)
Supplier: Merck Millipore (Oncogene)
Description: This Western-Re-Probe™ reagent is validated for use in immunoblotting.
UOM: 1 * 100 mL
Product Image-PIER35081

SuperSignal™, Pierce™ Fast Western Blotting Kit, West Femto

Supplier: Thermo Fisher Scientific
Description: The Pierce™ Fast Western Blot Kit, SuperSignal™ West Femto Substrate contains optimised reagents that shorten the time to perform a typical Western blot from 4 hours to ~60 minutes.

Product Image-786-124T

Sample clean up, Perfect-FOCUS™

Supplier: G-Biosciences
Description: Perfect-FOCUS™ is based on quantitative precipitation and concentration of protein solutions using UPPA™ (Universal Protein Precipitation Agent) and has been specifically developed for preparing lower conductivity protein samples for iso-electric focusing gels. Perfect-FOCUS™ is designed to clean and remove interfering agents from protein samples and concentrate protein solutions that give poor protein spot resolution during 2D electrophoresis.

Image Unavailable-J63209.AK

Saponin permeating solution, 0,5% w/v solution in PBS (10X)

Catalog Number: (J63209.AK)
Supplier: Thermo Fisher Scientific
Description: Saponin permeating solution, is used as a cell permeabilising agent.
UOM: 1 * 250 mL

MSDS Certificates


Image Unavailable-J61462.AK

Luminol and oxidising solutions

Supplier: Thermo Fisher Scientific
Description: For chemiluminecent HRP-based detection of western blotting systems. It is soluble in most polar organic solvents but is insoluble in water.

MSDS Certificates

Image Unavailable-J63618.AD

Prehybridisation solutions in SSC

Supplier: Thermo Fisher Scientific
Description: For the hybridization of probes to nucleic acids in Northern and Southern blotting.

MSDS Certificates

Product Image-PIER15159

Enhanced chemifluorescent HRP substrate kit, QuantaRed™

Catalog Number: (PIER15159)
Supplier: Thermo Fisher Scientific
Description: QuantaRed™ Enhanced Chemifluorescent HRP Substrate contains chemical enhancers to increase the fluorescent yield and sensitivity of 10-acetyl-3,7-dihydroxyphenoxazine (ADHP) chemifluorescence.
UOM: 1 * 1 items
Product Image-ROCKKCA001

Chemiluminescent western blot kits, FemtoMax™

Supplier: Rockland Immunochemicals
Description: These Chemiluminescent Western Blot Kits allow for the detection of primary polyclonal or monoclonal antibodies provided by the user. After protein separation and transfer, the membrane is probed with primary antibody. Detection of the membrane-bound primary antibody-antigen complex is achieved by the addition of a secondary antibody conjugated to the enzyme horseradish peroxidase. The enzyme reacts with a specialised formulation of luminol, an extremely sensitive, non radioactive substrate that emits light and allows visualisation using X-ray film or other imaging methods, including highly sensitive CCD cameras and imaging systems. Because of the extremely high sensitivity of the kit, primary and secondary antibodies can be used at greater dilutions. Primary antibodies currently diluted 1:1000 using an ECL™ substrate is the equivalent of a 1:5000 dilution using the FemtoMax™ substrate.

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on +353 1 88 22222.
Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on +353 1 88 22222
This product is marked as restricted and can only be purchased by approved Shipping Accounts. If you need further assistance, email VWR Regulatory Department at eurega_services@eu.vwr.com
-Additional Documentation May be needed to purchase this item. A VWR representative will contact you if needed.
This product has been blocked by your organisation. Please contact your purchasing department for more information.
The original product is no longer available. The replacement shown is available.
Product(s) marked with this symbol are discontinued - sold till end of stock. Alternatives may be available by searching with the VWR Catalog Number listed above. If you need further assistance, please call VWR Customer Service on +353 1 8822222.
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