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You Searched For: Western Blotting Reagents

Nucleic acids or proteins in a complex sample can be visualized using electrophoresis and blotting procedures and the compatible reagents. Improving the overall efficiency of electrophoresis systems, these reagents accommodate a variety of blotting techniques and with antibodies provided against tens of thousands of different proteins. These methods are used in the fields of molecular biology, immunogenetics, and other molecular biology disciplines.

Nucleic acids or proteins in a complex sample can be visualized using electrophoresis and blotting procedures and the compatible reagents. Improving the overall efficiency of electrophoresis systems, these reagents accommodate a variety of blotting techniques and with antibodies provided against tens of thousands of different proteins. These methods are used in the fields of molecular biology, immunogenetics, and other molecular biology disciplines.


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Product Image-786-372

Transfer pads for western blotting, Swift™

Supplier: G-Biosciences
Description: SWIFT™ transfer pads are treated with a proprietary electrolyte buffer to enhance Western blot transfer efficiency.

Product Image-TB54

Western blots, pre-made, human single tissue single species

Supplier: G-Biosciences
Description: Ready-to-screen, human, single tissue blots, from both normal and tumour tissues. G-Biosciences pre-made blots are prepared with the appropriate GenLysate™ and 50 μg protein is loaded onto a 4 to 20% denaturing polyacrylamide gel, along with a prestained molecular weight marker. After the proteins are resolved, they are transferred to a PVDF membrane, using our Western transfer buffer.

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Product Image-BB01

Botanical garden blot

Catalog Number: (BB01)
Supplier: G-Biosciences
Description: G-Biosciences' Botanical Garden Blot is prepared using total protein extracted from a variety of 5 to 10 day old, whole plant tissues.
UOM: 1 * 1 items
Product Image-TB57

Brain tissue region blots, single species

Supplier: G-Biosciences
Description: G-Biosciences offers ready-to-screen, normal tissue, brain tissue region blots. The brain tissue specific regions are prepared by carefully dissecting out anatomically and functionally distinct regions of adult mouse, rat, human, and primate brains.

Product Image-TB46

Heart tissue region blots, single species

Supplier: G-Biosciences
Description: These are ready to screen, normal tissue, heart tissue region blots from human and pig. The heart tissue specific regions are prepared by carefully dissecting out anatomically and functionally distinct regions of adult pig and adult human hearts.

Product Image-MILF64-202BP

Visualizer Western Blot Detection Kit

Supplier: Merck
Description: The Visualizer Western Blot Detection kit is designed for the sensitive detection of proteins in chemiluminescent Western blots.

Product Image-MILF2502

Chemicon® ReBlot Plus Mild Antibody Stripping Solution, 10X

Catalog Number: (MILF2502)
Supplier: Merck
Description: Western blotting is a commonly used technique for studying protein function and localisation. Typically, protein samples are electro-phoresed on SDS-page and transferred to a membrane such as nitrocellulose or nylon, where they are probed with specific antibodies. Unlike nucleic acid based technologies, which allow reuse of southern and northern blots, it has been difficult to reuse western blots.
UOM: 1 * 25 mL
Product Image-TB20

Mouse single tissue blot, single species

Supplier: G-Biosciences
Description: This blot contains two protein lanes, a protein marker lane and a lane with one GenLysate™ sample. After the proteins are resolved, they are transferred to a PVDF membrane using Western Transfer Buffer. The blots are ready to be blocked and probed with the antibodies of choice.

Product Image-TB03

Rat single tissue blot, single species

Supplier: G-Biosciences
Description: This blot contains two protein lanes, a protein marker lane and a lane with one GenLysate™ sample. After the proteins are resolved, they are transferred to a PVDF membrane using Western Transfer Buffer. The blots are ready to be blocked and probed with the antibodies of choice.

Product Image-MILF2060

Chemicon® ReBlot Western Blot Recycling Kit

Catalog Number: (MILF2060)
Supplier: Merck
Description: Western blotting is a commonly used technique for studying protein function and localisation. Typically, protein samples are electro-phoresed on SDS page and transferred to a membrane such as nitrocellulose or nylon, where they are probed with specific antibodies. Unlike nucleic acid based technologies, which allow reuse of southern and northern blots, it has been difficult to reuse western blots.
UOM: 1 * 1 items
Product Image-PIER34096

Protein immunodetection reagents, SuperSignal™ West Femto maximum sensitivity substrate

Supplier: Thermo Fisher Scientific
Description: SuperSignal™ West Femto maximum sensitivity substrate enables detection of low-femtogram amounts of protein deposited on nitrocellulose or PVDF membrane and probed with appropriate primary and secondary antibodies.

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Product Image-PIER46428

Western blot stripping buffer, restore PLUS™

Supplier: Thermo Fisher Scientific
Description: Some antibodies are difficult to remove from Western blots and require Longer incubation times or incubation temperatures greater than 22 °C. Restore™ PLUS Western Blot Stripping Buffer was developed to reduce incubation times while keeping incubations at room temperature. High-affinity antibodies can be quickly and effectively stripped from Western blots without removing transferred proteins, thereby allowing multiple reprobes of the target.

Product Image-PIER34035

Substrate for western blotting, NBT (nitro-blue tetrazolium chloride)

Catalog Number: (PIER34035)
Supplier: Thermo Fisher Scientific
Description: NBT (nitro-blue tetrazolium chloride) yields a blue-purple, water-insoluble product upon reduction of the tetrazolium salt. The product of reduction is NBT-formazan. This substrate is widely used for immunochemistry applications because the color is linear and stable. NBT is used as the substrate for both glucose oxidase and alkaline phosphatase systems.
UOM: 1 * 1 g
Product Image-PIER37552

Peroxidase conjugate stabiliser/diluent, Guardian™

Supplier: Thermo Fisher Scientific
Description: Guardian™ Peroxidase conjugate stabiliser/diluent preserves the functional integrity and activity of horseradish peroxidase conjugated antibodies and other proteins at very dilute concentrations for long term storage.

Product Image-PIER35055

Western blotting kit, ECL substrate, Pierce™

Supplier: Thermo Fisher Scientific
Description: The Fast Western Blot Kit, ECL Substrate contains optimised reagents that shorten the time which is required to perform a typical chemiluminescent Western blot from 4 hours down to approximately 55 minutes.

Product Image-PIER37574

1-Step™ Ultra TMB-Blotting solution, Pierce™

Catalog Number: (PIER37574)
Supplier: Thermo Fisher Scientific
Description: The Thermo Scientific 1-Step Ultra TMB-Blotting Solution is a ready-to-use, single-component horseradish peroxidase (HRP) substrate for Western blotting applications (not for use in ELISA assays or immunohistochemical applications). The high sensitivity, increased signal-to-noise ratio and low background blotting solution contains soluble TMB (3,3´,5,5´-tetramethylbenzidine), which reacts with HRP to produce an insoluble dark blue precipitate. The substrate is compatible with both nitrocellulose and PVDF membranes.
UOM: 1 * 250 mL
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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on +353 1 88 22222
This product is marked as restricted and can only be purchased by approved Shipping Accounts. If you need further assistance, email VWR Regulatory Department at eurega_services@eu.vwr.com
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This product has been blocked by your organisation. Please contact your purchasing department for more information.
The original product is no longer available. The replacement shown is available.
Product(s) marked with this symbol are discontinued - sold till end of stock. Alternatives may be available by searching with the VWR Catalog Number listed above. If you need further assistance, please call VWR Customer Service on +353 1 8822222.
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