Fetus Lysate (7 Day Fetus)

Supplier: ProSci Inc.

1451-F7
PRSI1451-F7EA 0 GBP
PRSI1451-F7
Fetus Lysate (7 Day Fetus)
Cells

Fetus lysate (7 Day Fetus) was prepared by homogenisation in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7,4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0,1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin.


Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6,8, 12,5% glycerol, 1% sodium dodecylsulfate, 0,01% bromophenol blue) containing 5% β-mercaptoethanol.

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