Fluor-de-Lys® SIRT1 fluorometric drug discovery assay kit

Supplier: ENZO LIFE SCIENCES

BML-AK555-0001 BMLAK5550001
ALEXBML-AK555-0001EA 852 GBP
ALEXBML-AK555-0001 ENZOBMLAK5550001
Fluor-de-Lys® SIRT1 fluorometric drug discovery assay kit
Assays Cellular Assays

A Fluor-de-Lys® fluorescent assay system. The SIRT1 Fluorescent Activity Assay/Drug Discovery Kit is a complete assay system designed to measure the lysyl deacetylase activity of the recombinant human SIRT1 included in the kit.


  • Useful for inhibitor screening or characterizing enzyme kinetics
  • Includes optimal substrate selected from a panel of acetylated sites in p53 and histones


The Fluor-de-Lys® SIRT1 assay is based on the Fluor de Lys® SIRT1 Substrate and Fluor de Lys® Developer II combination. The assay procedure has two steps. First, the Fluor de Lys® SIRT1 Substrate, which contains a peptide comprising amino acids 379-382 of human p53 (Arg-His-Lys-Lys(Ac)). Deacetylation of the substrate sensitizes the substrate so that, in the second step, treatment with the Fluor de Lys®Developer II produces a fluorophore.


Delivery information: Kit includes SIRT1 (Sirtuin 1, hSir2SIRT1) (human, recombinant), FLUOR DE LYS® SIRT1 Deacetylase Substrate, 5X FLUOR DE LYS® Developer II Concentrate, NAD (Sirtuin Substrate), Nicotinamide (Sirtuin Inhibitor), Resveratrol (Sirtuin Activator), Suramin sodium (Sirtuin Inhibitor), FLUOR DE LYS® Deacetylated Standard, Sirtuin Assay Buffer, Half volume microplates.

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