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pSTBlue-1 AccepTor™ vector kit

Description: The pSTBlue-1 AccepTor™ vector kits are designed to simplify the PCR cloning process by using non-proofreading thermostable DNA polymerases (eg. KOD XL polymerase and native and recombinant <i>Taq</i> polymerases), which leave single 3'-dA overhangs on the reaction products. The AccepTor™ vectors enable direct ligation by virtue of single 3'-dU overhangs that anneal efficiently with 3'-dA extensions on PCR products. The dU residues are converted to dT residues<i> in vivo</i> following transformation.
Catalog Number: 70595-4
UOM: 1 * 40 Tests
Supplier: Merck Millipore (Novagen)
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IPTG (isopropyl-β-D-thiogalactopyranoside) solution 100 mM, Sigma-Aldrich®

Description: The lac operon inducer IPTG (isopropyl-β-D- 1-thiogalactopyranoside) is recommended for blue/white screening by lacZ α- complementation with appropriate vectors and host strains, such as pSTBlue-1/NovaBlue, and for inducing protein expression with the pET System and other lac promoter-derived expression systems.
Catalog Number: 70527-3
UOM: 1 * 15 mL
Supplier: Merck Millipore (Novagen)
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Protein extraction kits, BugBuster® Plus Lysonase™

Description: BugBuster® Plus Lysonase™ includes BugBuster Protein Extraction Reagent and Lysonase Bioprocessing Reagent, an optimised, ready-to-use blend of rLysozyme™ and Benzonase® Nuclease in a single kit. This powerful combination of two reagents allows maximum recovery of active soluble protein from both Gram-negative and Gram-positive bacteria. The BugBuster® and rLysozyme™ efficiently lyse the bacterial cells while the Benzonase® degrades nucleic acids to facilitate downstream processing. Requires 5 ml BugBuster and 10 µl Lysonase per 1 g cell paste. The kits provide sufficient reagents for protein extraction from 20 g or 100 g cell paste.
Catalog Number: 71370-3
UOM: 1 * 1 items
Supplier: Merck Millipore (Novagen)
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Protein transfection reagent, Novagen®, ProteoJuice™

Description: ProteoJuice™ protein transfection reagent is an effective reagent for the transfection of intact functional protein and peptides into mammalian cells with minimal toxicity and broad cell specificity.
Catalog Number: 71281-3
UOM: 1 * 1 items
Supplier: Merck Millipore (Novagen)
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Protein extraction reagent, BugBuster® HT, Millipore®

Description: BugBuster® HT Protein Extraction Reagent combines BugBuster® Protein Extraction Reagent and Benzonase® Nuclease in one convenient reagent. BugBuster® HT eliminates common bioprocessing problems resulting from traditional cell lysis procedures. Soluble proteins are gently extracted from <i>E. coli</i> without exposure to heat or oxidative damage and viscosity is eliminated by nucleic acid digestion in a single step. The resulting protein extract can easily be fractionated by conventional purification techniques. BugBuster HT is ideally suited for application in high throughput protein purifications.
Catalog Number: 70922-3
UOM: 1 * 100 mL
Supplier: Merck Millipore (Novagen)
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Bovine Ribonuclease A (from Pancreas)

Description: Convenient solution for selective degradation of RNA.
Catalog Number: 70856-3
UOM: 1 * 1 mL
Supplier: Merck Millipore (Novagen)
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Dialysers, D-Tube™ and D-Tube96™

Description: D-Tube96™ Dialysers allow convenient, high throughput dialysis of 96 samples simultaneously. The device features the advantages of the D-Tube™ Dialyzer Mini in a 96-tube format. D-Tube Dialysers are easy to handle tubes with dual membranes providing a large surface area for fast, efficient dialysis. The membrane is ultra clean, EDTA-treated, regenerated cellulose that is sulfur and heavy metal-free.
Catalog Number: 71713-3
UOM: 1 * 1 items
Supplier: Merck Millipore (Novagen)
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His·Bind® buffer kit, Novagen®

Description: The His·Bind buffer kit is a set of pre-tested buffers designed for use with IDA-based His·Bind resin for convenient, rapid one-step purification of proteins by metal chelation chromatography.
Catalog Number: 69755-3
UOM: 1 * 1 KIT
Supplier: Merck Millipore (Novagen)
Product Image-69750-3

pET-24b(+) DNA, Novagen®

Description: The pET-24a-d(+) vectors carry an N-terminal T7-Tag® sequence plus an optional C-terminal His-Tag® sequence. These vectors differ from pET-21a-d(+) only by their selectable marker (kanamycin vs ampicillin resistance).
Catalog Number: 69750-3
UOM: 1 * 10 µG
Supplier: Merck Millipore (Novagen)
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S-protein agarose, Novagen

Description: S-protein agarose specifically retains STag™ fusion proteins. The capacity of S-protein agarose varies and is based on the size and folding characteristics of a given target protein.
Catalog Number: 69704-4
UOM: 1 * 10 mL
Supplier: Merck Millipore (Novagen)
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Competent cells, expression strains, BLR

Description: A common method for transformation of plasmids into E.coli is the use of chemically competent cells. Although competent cells can be prepared in the laboratory, greater efficiency, reproducibility, and convenience are achieved using Novagen® prepared competent cells. Every Novagen® competent cell strain is verified for phenotype and purity, and is guaranteed for transformation efficiency.
Catalog Number: 69053-3
UOM: 1 * 0,4 mL
Supplier: Merck Millipore (Novagen)
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pET-30a(+) DNA, Novagen®

Description: The pET-30a-c(+) vectors carry an N-terminal His Tag®/thrombin/S Tag™/enterokinase configuration plus an optional C-terminal His tag sequence.
Catalog Number: 69909-3
UOM: 1 * 10 µG
Supplier: Merck Millipore (Novagen)
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PopCulture® reagent

Description: PopCulture® Reagent is a detergent-based concentrate that can be added directly to cultures of <i>E. coli</i> to effectively extract proteins without the need for cell harvest. Recombinant proteins can be directly screened in the crude extract, or purified by adding an affinity matrix, washing the matrix-target protein complex to remove spent culture medium and cellular contaminants, and eluting the purified protein from the matrix. The PopCulture protein purification procedure is ideally suited to high-throughput robotic processing of samples for proteomics research or any application that would benefit from the increased speed and convenience.
Catalog Number: 71092-3
UOM: 1 * 15 mL
Supplier: Merck Millipore (Novagen)
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Expression vectors, pET-41 DNA, Novagen®

Description: pET-41 series is designed for cloning and high-level expression of peptide sequences fused with the 220 aa GST•Tag™ protein. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase. The f1 origin is oriented so that infection with helper phage will produce virions containing single stranded DNA that corresponds to the coding strand.
Catalog Number: 70556-3
UOM: 1 * 10 µG
Supplier: Merck Millipore (Novagen)
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BugBuster® Ni-NTA His•Bind® purification kit

Description: BugBuster®Ni-NTA HIS-BIND® Purification Kit is used for protein purification. Ni-NTA HIS-BIND® Resin is high-performance Ni2+-charged agarose used for rapid, one-step purification of proteins containing a polyhistidine tag sequence.
Catalog Number: 70793-3
UOM: 1 * 1 KIT
Supplier: Merck Millipore (Novagen)
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Ni-NTA His-Bind® Superflow™ Resin

Description: Ni-NTA HisBind® Superflow™ Resin is a medium-pressure compatible version of the Ni-NTA HisBind® Resin
Catalog Number: 70691-5
UOM: 1 * 100 mL
Supplier: Merck Millipore (Novagen)
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