You Searched For: Quantabio

Supplier: Quantabio

Description: The AccuStart™ II Mouse Genotyping kit is designed for fast and easy preparation of PCR-ready DNA extracts and endpoint PCR analysis from tissues such as tail snips and ear punches commonly used for genotyping knockout and transgenic animals. The kit combines Extracta™ DNA Prep for PCR - Tissue with AccuStart™ II GelTrack™ PCR SuperMix in a single kit.

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Supplier: Quantabio

Description: Faster, longer, tougher next gen cDNA synthesis with flexible priming.

Supplier: Quantabio

Description: Ultra-Plex™ 1-Step ToughMix® is a ready-to-use, 4X-concentrated master mix for reverse transcription quantitative PCR (RT-qPCR) of RNA templates using hybridisation probe detection chemistries such as TaqMan® 5’-hydrolysis probes on Real-Time PCR systems that utilise ROX passive reference with 490 nM excitation. First-strand cDNA synthesis and PCR amplification are carried out in the same tube without opening between procedures. It is ideal for highly sensitive quantification of RNA viruses or low abundance RNA targets in uni- or multiplexed RT-qPCR applications as well as high throughput gene-expression studies. The system has been optimised to deliver maximum RT-qPCR efficiency, sensitivity, and specificity in reduced reaction volumes and fast cycle times. Ultra-Plex™ 1-Step ToughMix® contains all required components for RT-qPCR except RNA template and probe. It is compatible with all dual-labeled probe chemistries.

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Supplier: Quantabio

Description: PerfeCTa® MultiPlex qPCR ToughMix® is a 5X concentrated, ready to use reaction cocktail for Real-Time quantitative PCR (qPCR) that overcomes many known inhibitors of PCR often present in crude samples extracted from environmental specimens, plant tissues, or animal tissues. It contains all components, except primers, probes and templates. The 5X concentrated ToughMix® allows addition of higher amounts of template and improved detection sensitivity with low concentration samples. PerfeCta® MultiPlex qPCR ToughMix® has been optimised to deliver maximum PCR efficiency, sensitivity, and specificity in reduced reaction volumes with fast cycle or conventional PCR cycling protocols.

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Supplier: Quantabio

Description: The qScript™ XLT One-Step RT-PCR Kit is a convenient and highly sensitive system for amplification of RNA templates up to 2 kb. cDNA synthesis and PCR amplification are carried out in the same tube without opening between procedures. This system has been optimised to deliver maximum endpoint RT-PCR efficiency, sensitivity, and specificity.

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Supplier: Quantabio

Description: AccuStart™ II PCR SuperMix is a 2X concentrated, ready-to-use reaction cocktail for routine PCR amplification of DNA fragments up to 4 kb. It contains all components, except primers and template. AccuStart™ II PCR SuperMix simplifies reaction assembly, improves assay reproducibility, and reduces the risk of contamination. A key component is AccuStart™ II Taq DNA polymerase which contains monoclonal antibodies that bind to the polymerase and keep it inactive prior to the initial PCR denaturation step. Upon heat activation (1 minute at 94 °C), the antibodies denature irreversibly, releasing fully active, unmodified Taq DNA polymerase. This enables specific and efficient primer extension with the convenience of room temperature reaction assembly.

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Supplier: Quantabio

Description: PerfeCTa® qPCR SuperMixes are 2X concentrated, ready to use reaction cocktails containing all components except primers, probe and template for qPCR.

Supplier: Quantabio

Description: AccuStart™ II <i>Taq</i> DNA Polymerase is a high purity, recombinant <i>Taq</i> DNA polymerase preparation with high avidity monoclonal antibodies that bind the polymerase and keep it inactive prior to the initial PCR denaturation step. Upon heat activation (1 minute at 94 °C), the antibodies denature irreversibly, releasing fully active, unmodified <i>Taq</i> DNA polymerase. This enables specific and efficient primer extension with the convenience of room temperature reaction assembly. Non-specific extension of primers at low temperatures is a common cause of artifacts and poor sensitivity in PCR. The AccuStart™ II automatic hot-start enables specific and efficient primer extension in the PCR process with the added convenience of room temperature reaction assembly. The included 10X PCR Buffer II is a new optimised buffer that provides higher product yield, improved specificity, and enhanced multiplexing capability.

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Supplier: Quantabio

Description: FastMixes include buffers and stabilisers optimised for maximum PCR efficiency, reproducibility, and sensitivity with TaqMan® and TaqMan® MGB probe chemistries. A key component of PerfeCTa® qPCR FastMixes is AccuFast™ <i>Taq</i> DNA polymerase that enables instant activation and rapid polymerisation kinetics. Upon heat activation (20 seconds at 95 °C), the antibodies irreversibly denature releasing fully active, unmodified <i>Taq</i> DNA polymerase. This promotes specific and efficient primer extension with the convenience of room temperature reaction assembly. FastMixes deliver maximum PCR efficiency, sensitivity, specificity, and a robust fluorescent signal with either fast or conventional cycling conditions.

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Catalog Number: (733-1271)

Supplier: Quantabio

Description: The 10 mM dNTP mix is a solution of high purity deoxyribonucleoside triphosphates that has been functionally qualified for real-time quantitative PCR (qPCR). It is suitable for use in conventional end-point PCR, real time qPCR, first-strand cDNA synthesis, as well as other applications that require dNTP as substrate. The use of qPCR certified dNTPs is a simple but important measure to ensure consistent assay performance.

UOM: 1 * 1.000 µl

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Supplier: Quantabio

Description: This SuperMix is a 2X concentrated, ready to use reaction cocktail for Real-Time qPCR that contains all components, except primers, probes, and templates. The system transcends multiplex limitations of conventional PCR master mixes, enabling unbiased amplification of up to five target sequences in a single tube. Suppression of low copy amplicons by high copy reference targets in the amplification is a common problem in multiplex PCR. This can skew, or mask the apparent representation and quantification of low copy target sequences. PerfeCTa® MultiPlex qPCR SuperMix delivers dynamic range and sensitivity to multiplexed qPCR that is comparable to that for singleplex qPCR probe assays without the need for limiting or variable primer concentrations.

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Supplier: Quantabio

Description: Ultra FAST RNA library prep with integrated rRNA and globin depletion.

Catalog Number: (QUNT95900-4CEXTWAR)

Supplier: Quantabio

Description: Q Instrument extended warranty program.

UOM: 1 * 1 items

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Supplier: Quantabio

Description: Rapid extraction and purification of high-quality total RNA from cultured cells or tissue.

Supplier: Quantabio

Description: qScript™ XLT cDNA SuperMix is a next generation tool for first-strand cDNA synthesis, providing a highly sensitive and easy to use solution for two-step RT-PCR and RT-qPCR. qScript XLT is an engineered M-MLV reverse transcriptase with reduced RNase H activity and improved activity and stability at higher temperatures. Combined with a precise mixture of reaction components, this SuperMix enables superior results over a wide dynamic range of input RNA, with up to 8-fold higher sensitivity than cDNA synthesis kits utilising an RNase H(+) reverse transcriptase (RT).

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Supplier: Quantabio

Description: AccuStart™ Genotyping ToughMix® is a 2X concentrated ready to use reaction cocktail for PCR amplification of DNA templates that overcomes inhibitors often present in crude samples extracted from environmental specimens, plant tissues, or animal tissues. It is a versatile and robust PCR reagent that provides highly specific and efficient amplification for genotyping sequence variants including allelic discrimination of single nucleotide polymorphisms (SNPs) using a variety of fluorogenic probe chemistries, including TaqMan® hydrolysis probes.

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