You Searched For: Thermo Fisher Scientific

Supplier: Thermo Fisher Scientific

Description: The Pierce™ S-Nitrosylation Western Blot Kit enables sensitive detection of protein S-nitrosocysteine post-translational modifications.

Catalog Number: (PIERA39268)

Supplier: Thermo Fisher Scientific

Description: Thermo Scientific Pierce Sulfo-SMCC, No-Weigh Format is a water-soluble, amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a medium-length cyclohexane spacer arm (8.3 angstroms).

UOM: 1 * 20 mg

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Catalog Number: (PIERA35349)

Supplier: Thermo Fisher Scientific

Description: Thermo Scientific Pierce TCEP-HCl is a potent, versatile, odorless, thiol-free reducing agent with broad application to protein and other research involving reduction of disulfide bonds. The unique compound is easily soluble and very stable in many aqueous solutions. TCEP reduces disulfide bonds as effectively as dithiothreitol (DTT), but unlike DTT and other thiol-containing reducing agents, TCEP does not have to be removed before certain sulfhydryl-reactive crosslinking reactions.

UOM: 1 * 10 mg

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Catalog Number: (PIERA35394)

Supplier: Thermo Fisher Scientific

Description: Thermo Scientific Pierce SMCC is a hetero-bifunctional crosslinker that contain N-hydroxysuccinimide (NHS) ester and maleimide groups that allow covalent conjugation of amine- and sulfhydryl-containing molecules. NHS esters react with primary amines at pH 7–9 to form amide bonds, while maleimides react with sulfhydryl groups at pH 6.5–7.5 to form stable thioether bonds. In aqueous solutions, NHS ester hydrolytic degradation is a competing reaction whose rate increases with pH. The maleimide group is more stable than the NHS-ester group, but will slowly hydrolyze and lose its reaction specificity for sulfhydryls at pH values > 7.5. For these reasons, conjugations with these crosslinkers are usually performed at pH 7.2–7.5, with the NHS ester (amine-targeted) reacted before or simultaneous with the maleimide (sulfhydryl-targeted) reaction.

UOM: 1 * 10 mg

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Supplier: Thermo Fisher Scientific

Description: Thermo Scientific EZ-Link Sulfo-NHS-Biotin is a short-chain, water-soluble biotinylation reagent for labeling antibodies, proteins and other molecules that have primary amines.

Supplier: Thermo Fisher Scientific

Description: Pierce™ Streptavidin is a purified unconjugated recombinant protein.

Catalog Number: (PIER24585)

Supplier: Thermo Fisher Scientific

Description: The Thermo Scientific Pierce reversible protein stain kit for PVDF membranes is a rapid and sensitive alternative to ponceau S stain for protein detection on PVDF membrane after transfer from polyacrylamide gels.

UOM: 1 * 1 KIT

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Catalog Number: (PIER21578)

Supplier: Thermo Fisher Scientific

Description: Thermo Scientific Pierce DTSSP is a popular water-soluble crosslinker that contains amine-reactive NHS-ester ends around an 8-atom spacer arm, whose central disulfide bond can be cleaved with reducing agents.

UOM: 1 * 50 mg

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Supplier: Thermo Fisher Scientific

Description: Thermo Scientific Pierce BS3 (Sulfo-DSS) is bis(sulfosuccinimidyl)suberate, an amine-to-amine crosslinker that is homobifunctional, water-soluble, non-cleavable and membrane impermeable.

Supplier: Thermo Fisher Scientific

Description: Thermo Scientific Pierce TCEP-HCl is a potent, versatile, odorless, thiol-free reducing agent with broad application to protein and other research involving reduction of disulfide bonds. The unique compound is easily soluble and very stable in many aqueous solutions. TCEP reduces disulfide bonds as effectively as dithiothreitol (DTT), but unlike DTT and other thiol-containing reducing agents, TCEP does not have to be removed before certain sulfhydryl-reactive crosslinking reactions.

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Catalog Number: (PIER20308)

Supplier: Thermo Fisher Scientific

Description: SDS-Out™ SDS Precipitation Kit provides convenient removal of SDS from small-volume (0,1 to 10 ml) protein samples.

UOM: 1 * 1 KIT

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Catalog Number: (PIER21120)

Supplier: Thermo Fisher Scientific

Description: Pierce™ Hydrazide-activated strepavidin conjugate includes recombinant streptavidin in a purified form activated for crosslinking to carbonyl groups in a molecule.

UOM: 1 * 2 mg

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Catalog Number: (PIER17856)

Supplier: Thermo Fisher Scientific

Description: Agarose II (low melt) is a highly purified and carefully blended formulation that provides uniform lot-to-lot consistency for preparation of electrophoresis gels that require a lower melting temperature.

UOM: 1 * 100 g

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Supplier: Thermo Fisher Scientific

Description: These Ni(2+) chelate-coated plates are ideal for analysing polyhistidine-tagged fusion proteins by ELISA-based methods. Proteins that contain a succession of several histidine residues at the amino or carboxyl terminus have a strong binding affinity for metal. Bacterial lysates containing polyhistidine-tagged fusion proteins can be added directly to the plates without the need for blocking. The clear, white or black plates can be used with colorimetric, chemiluminescent or fluorescent detection methods, respectively.

Catalog Number: (PIER29810)

Supplier: Thermo Fisher Scientific

Description: Ethylene glycol

UOM: 1 * 200 mL

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Catalog Number: (PIER22329)

Supplier: Thermo Fisher Scientific

Description: Thermo Scientific Pierce SIAB is a mid-length (10.6 angstrom) crosslinker for amine-to-sulfhydryl conjugation via N-hydroxysuccinimide (NHS) ester and iodoacetyl reactive groups.

UOM: 1 * 50 mg

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