You Searched For: Thermo Fisher Scientific

Catalog Number: (PIER44688)

Supplier: Thermo Fisher Scientific

Description: Pierce™ F(ab')2 micro preparation kit enables efficient Fab generation from IgG.

UOM: 1 * 1 KIT

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Supplier: Thermo Fisher Scientific

Description: Thermo Scientific Pierce TCEP-HCl is a potent, versatile, odorless, thiol-free reducing agent with broad application to protein and other research involving reduction of disulfide bonds. The unique compound is easily soluble and very stable in many aqueous solutions. TCEP reduces disulfide bonds as effectively as dithiothreitol (DTT), but unlike DTT and other thiol-containing reducing agents, TCEP does not have to be removed before certain sulfhydryl-reactive crosslinking reactions.

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Supplier: Thermo Fisher Scientific

Description: These Ni(2+) chelate-coated plates are ideal for analysing polyhistidine-tagged fusion proteins by ELISA-based methods. Proteins that contain a succession of several histidine residues at the amino or carboxyl terminus have a strong binding affinity for metal. Bacterial lysates containing polyhistidine-tagged fusion proteins can be added directly to the plates without the need for blocking. The clear, white or black plates can be used with colorimetric, chemiluminescent or fluorescent detection methods, respectively.

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Supplier: Thermo Fisher Scientific

Description: This BCA Protein Assay Kit - Reducing Agent Compatible enables the measurement of protein concentration in samples that contain thiol-reductants dithiothreitol (DTT) and 2-mercaptoethanol (BME).

Supplier: Thermo Fisher Scientific

Description: Thermo Scientific Pierce BS3 (Sulfo-DSS) is bis(sulfosuccinimidyl)suberate, an amine-to-amine crosslinker that is homobifunctional, water-soluble, non-cleavable and membrane impermeable.

Supplier: Thermo Fisher Scientific

Description: Pierce

Supplier: Thermo Fisher Scientific

Description: Cross linkers are used for the identification of near-neighbour protein relationships and ligand-receptor interactions. Homobifunctional, amine-reactive NHS esters or imidates, and heterobifunctional, amine-reactive, photoactivatable phenyl azides are the most commonly used crosslinkers for these applications.

Supplier: Thermo Fisher Scientific

Description: The Pierce™ S-Nitrosylation Western Blot Kit enables sensitive detection of protein S-nitrosocysteine post-translational modifications.

Supplier: Thermo Fisher Scientific

Description: Desthiobiotin-ATP probe, 16×12,6 µg

Supplier: Thermo Fisher Scientific

Description: The Active GTPase Pull-Down and Detection Kits allows the monitoring of changes in GTPase activity levels by enriching and detecting the active (GTP-bound) state of a particular GTPase.

Catalog Number: (PIER28300)

Supplier: Thermo Fisher Scientific

Description: 3-[(3-Cholamidopropyl)dimethylammonio]-1-propane sulphate (CHAPS)

UOM: 1 * 5 g

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Supplier: Thermo Fisher Scientific

Description: Pierce™ HRP conjugated avidin is used in biotin-based detection methods, including Western blotting or ELISA detection with appropriate enzyme substrates.

Catalog Number: (PIER37615)

Supplier: Thermo Fisher Scientific

Description: The 1-Step™ ABTS (2,2'-azinobis [3-ethylbenzthiazoline-6-sulfonic acid] diammonium salt) is a ready-to-use, water-soluble ELISA substrate for horseradish peroxidase. This substrate yields a green product with absorbance maxima at 410 nm and 650 nm. Adding SDS to the substrate stops the reaction without changing the colour.

UOM: 1 * 250 mL

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Catalog Number: (PIER32400)

Supplier: Thermo Fisher Scientific

Description: Pierce™ Recombinant Peroxidase Conjugated Protein A can be used to probe and detect rabbit and human antibodies, especially IgG isotypes, in Western blotting, ELISA, IHC and other immunoassay protocols.

UOM: 1 * 1 mg

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Supplier: Thermo Fisher Scientific

Description: Nitrocellulose membranes are a popular matrix used in protein blotting because of their high protein-binding affinity, compatibility with a variety of detection methods (chemiluminescence, chromogenic, and fluorescence), and the ability to immobilise proteins, glycoproteins, or nucleic acids. Protein immobilisation is thought to occur by hydrophobic interactions, and high salt and low methanol concentrations help improve protein immobilisation to the membrane during electrophoretic transfer, especially for proteins with higher molecular weights. Nitrocellulose membranes are not optimal for electrophoretic transfer of nucleic acids, as the high salt concentrations that are required for efficient binding will effectively elute some or all of the charged nucleic acid fragments.

Supplier: Thermo Fisher Scientific

Description: Pierce C18 spin tips enable fast and efficient capture, concentration, desalting, and elution of up to 10 µg of peptides per 20 µl of sample with volumes ranging from 10 to 100 µl.