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Product Image-F-530L

Phusion® High-Fidelity DNA Polymerase

Description: Phusion® High Fidelity DNA Polymerase generates PCR products with an accuracy and speed unattainable with a single enzyme, even on the most difficult templates. The structure and characteristics of Phusion® High Fidelity DNA Polymerase make it a superior choice for cloning, and sets the standard for PCR performance with an error rate 50-fold lower than that of <i>Taq</i> DNA polymerase, and 6-fold lower than that of <i>Pfu</i> DNA polymerase. In addition, Phusion® High Fidelity DNA Polymerase possesses processivity 10-fold greater than <i>Pfu </i>DNA polymerase.
Catalog Number: F-530L
UOM: 1 * 1 KIT
Supplier: FINNZYMES REAGENTS

MSDS


Product Image-F-130WH

Direct PCR kits

Description: Direct PCR Kits save time and cost by allowing amplification of DNA directly from the source material. Direct PCR is based on Thermo Scientific Phire® Hot Start II DNA Polymerase and Phusion® Hot Start II High-Fidelity DNA Polymerase. These fusion enzymes are exceptionally fast, robust and highly tolerant of many PCR inhibitors present in unpurified sample material. This allows reliable amplification in challenging conditions where conventional DNA polymerases are completely inhibited.
Catalog Number: F-130WH
UOM: 1 * 1 KIT
Supplier: FINNZYMES REAGENTS
Product Image-F-124L

Phire® Green Hot Start II DNA Polymerase

Description: Phire® Hot Start II DNA Polymerase is faster, extremely robust, and capable of amplifying long DNA fragments with high yields. Phire® Hot Start II DNA Polymerase incorporates a dsDNA-binding domain which allows short extension times (10 to 15 s/kb), improves yields, and increases fidelity 2-fold compared to <i>Taq</i> DNA polymerase. In addition, the Hot Start technology allows complete reactivation of the enzyme in “zero-time” at standard cycling temperatures. This combination of features makes the polymerase an ideal solution for routine and high throughput PCR applications. Phire® Hot Start II DNA Polymerase delivers superior performance in conventional thermal cyclers as well as in fast instruments, such as the Piko® Thermal Cycler.
Catalog Number: F-124L
UOM: 1 * 1 KIT
Supplier: FINNZYMES REAGENTS
Product Image-F-175L

Phusion® blood direct PCR master mix

Description: Phusion® Blood Direct PCR Master Mix is designed for amplification of DNA from whole blood. It eliminates the need for a separate DNA purification step prior to PCR. The modified Phusion® Hot Start II High-Fidelity DNA Polymerase is resistant to main PCR inhibitors present in blood and retains its activity at blood concentrations of up to 40% in the PCR reaction.
Catalog Number: F-175L
UOM: 1 * 1 KIT
Supplier: FINNZYMES REAGENTS
Product Image-F-122S

Phire® Hot Start II DNA polymerase

Description: Phire® Hot Start II DNA Polymerase is faster, extremely robust, and capable of amplifying long DNA fragments with high yields. Phire® Hot Start II DNA Polymerase incorporates a dsDNA-binding domain which allows short extension times (10 to 15 s/kb), improves yields, and increases fidelity 2-fold compared to <i>Taq</i> DNA polymerase. In addition, the Hot Start technology allows complete reactivation of the enzyme in “zero-time” at standard cycling temperatures. This combination of features makes the polymerase an ideal solution for routine and high throughput PCR applications. Phire® Hot Start II DNA Polymerase delivers superior performance in conventional thermal cyclers as well as in fast instruments, such as the Piko® Thermal Cycler.
Catalog Number: F-122S
UOM: 1 * 1 KIT
Supplier: FINNZYMES REAGENTS
Product Image-F-562L

Master mixes, multiplex, Phusion® U

Description: Phusion® U multiplex PCR master mix is a ready-to-use, 2X end-point PCR master mix designed for simultaneous amplification of multiple targets in a single tube.
Catalog Number: F-562L
UOM: 1 * 1 KIT
Supplier: FINNZYMES REAGENTS
Product Image-F-565S

DNA polymerases, Phusion® hot start II high-fidelity PCR master mix

Description: Phusion® high-fidelity DNA polymerases set a gold standard for high performance PCR.
Catalog Number: F-565S
UOM: 1 * 1 KIT
Supplier: FINNZYMES REAGENTS
Product Image-F-170S

Tissue direct PCR master mix, Phire™

Description: Phire™ Tissue Direct PCR Master Mix has been developed for amplification of DNA directly from a wide variety of tissues obtained from mice, human, fish, birds and insects. The master mix containing Phire™ Hot Start II DNA polymerase is specially formulated to perform PCR in the presence of different animal tissue-derived inhibitors, such as collagen, melanin and eumelanin (hair, skin) or myoglobin (muscle).
Catalog Number: F-170S
UOM: 1 * 1 KIT
Supplier: FINNZYMES REAGENTS
Product Image-F-532S

Phusion® High-Fidelity PCR Master Mix with GC Buffer

Description: Phusion® High-Fidelity PCR Master Mix with GC Buffer is a convenient 2X mix containing Phusion® DNA polymerase, nucleotides and optimised GC buffer including MgCl₂. Only template and primers need to be added by the user.
Catalog Number: F-532S
UOM: 1 * 1 KIT
Supplier: FINNZYMES REAGENTS
Product Image-F-541

Phusion® site-directed mutagenesis kit

Description: Phusion® Site-Directed Mutagenesis Kit is a versatile and efficient tool for introducing point mutations, insertions, or deletions in any type of plasmid DNA.
Catalog Number: F-541
UOM: 1 * 1 KIT
Supplier: FINNZYMES REAGENTS
Product Image-F-555L

DNA polymerases, Phusion® U hot start

Description: Phusion® U DNA polymerase is a novel engineered high fidelity enzyme developed using fusion technology.
Catalog Number: F-555L
UOM: 1 * 1 KIT
Supplier: FINNZYMES REAGENTS
Product Image-F-531L

Phusion® High-Fidelity PCR Master Mix with HF Buffer

Description: Phusion® High-Fidelity PCR Master Mix with HF Buffer is a convenient 2X mix containing Phusion® DNA Polymerase, nucleotides and optimised HF buffer including MgCl₂. Only template and primers need to be added by the user.
Catalog Number: F-531L
UOM: 1 * 1 KIT
Supplier: FINNZYMES REAGENTS
Product Image-F-549L

Phusion® Hot Start II high-fidelity DNA polymerase

Description: Phusion® Hot Start II High-Fidelity DNA Polymerase allows the use of a wide range of primers, including those with low melting temperatures. Phusion® Hot Start II High-Fidelity DNA Polymerase is ideal for PCR applications from routine PCR to highly demanding applications, such as cloning and high throughput PCR. It incorporates a unique dsDNA-binding domain making this Pyrococcus-like proofreading DNA polymerase extremely robust, rapid, and accurate.
Catalog Number: F-549L
UOM: 1 * 1 KIT
Supplier: FINNZYMES REAGENTS
Product Image-F-537L

Phusion® Green hot start II High-fidelity DNA polymerase

Description: Phusion® Green Hot Start II High-Fidelity DNA Polymerase is a combination of Phusion® Hot Start II DNA Polymerase and 5× Phusion® Green buffers. The buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel. The coloured buffer does not interfere with excellent performance of Phusion® Hot Start II DNA polymerase and is compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.
Catalog Number: F-537L
UOM: 1 * 1 KIT
Supplier: FINNZYMES REAGENTS
Product Image-F-556S

DNA polymerases, Phusion® green U hot start

Description: Phusion® U DNA polymerase is a novel engineered high fidelity enzyme developed using fusion technology.
Catalog Number: F-556S
UOM: 1 * 1 KIT
Supplier: FINNZYMES REAGENTS
Product Image-F-415XL

DyNAmo™ Flash SYBR® green qPCR kits

Description: DyNAmo™ qPCR Kits offer excellent performance in detection and quantitation of DNA and RNA sequences from various sources. The DyNAmo™ qPCR kit family features optimised kits for fast and conventional qPCR. Kits utilising either SYBR® Green or probe chemistries for various platforms are available. All DyNAmo™ qPCR kits are provided as convenient 2X master mixes - only template and primers need to be added.
Catalog Number: F-415XL
UOM: 1 * 1 KIT
Supplier: FINNZYMES REAGENTS
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