You Searched For: FINNZYMES REAGENTS

Supplier: FINNZYMES REAGENTS

Description: Phusion® High Fidelity DNA Polymerase generates PCR products with an accuracy and speed unattainable with a single enzyme, even on the most difficult templates. The structure and characteristics of Phusion® High Fidelity DNA Polymerase make it a superior choice for cloning, and sets the standard for PCR performance with an error rate 50-fold lower than that of <i>Taq</i> DNA polymerase, and 6-fold lower than that of <i>Pfu</i> DNA polymerase. In addition, Phusion® High Fidelity DNA Polymerase possesses processivity 10-fold greater than <i>Pfu </i>DNA polymerase.

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Supplier: FINNZYMES REAGENTS

Description: Phusion® U DNA polymerase is a novel engineered high fidelity enzyme developed using fusion technology.

Supplier: FINNZYMES REAGENTS

Description: Phusion® Hot Start II High-Fidelity DNA Polymerase allows the use of a wide range of primers, including those with low melting temperatures. Phusion® Hot Start II High-Fidelity DNA Polymerase is ideal for PCR applications from routine PCR to highly demanding applications, such as cloning and high throughput PCR. It incorporates a unique dsDNA-binding domain making this Pyrococcus-like proofreading DNA polymerase extremely robust, rapid, and accurate.

Supplier: FINNZYMES REAGENTS

Description: Phusion® Green Hot Start II High-Fidelity DNA Polymerase is a combination of Phusion® Hot Start II DNA Polymerase and 5× Phusion® Green buffers. The buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel. The coloured buffer does not interfere with excellent performance of Phusion® Hot Start II DNA polymerase and is compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.

Supplier: FINNZYMES REAGENTS

Description: DyNAmo™ qPCR Kits offer excellent performance in detection and quantitation of DNA and RNA sequences from various sources. The DyNAmo™ qPCR kit family features optimised kits for fast and conventional qPCR. Kits utilising either SYBR® Green or probe chemistries for various platforms are available. All DyNAmo™ qPCR kits are provided as convenient 2X master mixes - only template and primers need to be added.

Supplier: FINNZYMES REAGENTS

Description: Phusion® U hot start PCR master mix is a novel engineered high fidelity enzyme developed using fusion technology.

Supplier: FINNZYMES REAGENTS

Description: Phusion® green hot start II high-fidelity PCR master mix is a convenient 2X mix designed to minimise the number of pipetting steps. The master mix contains Phusion Hot Start II DNA Polymerase, nucleotides and optimised reaction buffer including MgCl₂. The buffer also includes a density reagent and two tracking dyes for direct loading of PCR products on a gel.

Supplier: FINNZYMES REAGENTS

Description: DyNAmo™ qPCR Kits offer excellent performance in detection and quantitation of DNA and RNA sequences from various sources. The DyNAmo™ qPCR kit family features optimised kits for fast and conventional qPCR. Kits utilising either SYBR® Green or probe chemistries for various platforms are available. All DyNAmo™ qPCR kits are provided as convenient 2X master mixes - only template and primers need to be added.

Supplier: FINNZYMES REAGENTS

Description: Phusion® High-Fidelity PCR Kit offers extreme performance for all major PCR applications. Incorporating Phusion® DNA polymerase, the Phusion® High-Fidelity PCR Kit contains all the reagents necessary, including lambda DNA control template and primers for 1,3 kb and 10 kb amplicons. The template amount is sufficient for performing 20 control reactions in 50 μl volume or 50 control reactions in 20 μl volume.

Supplier: FINNZYMES REAGENTS

Description: Phusion® U green multiplex PCR master mix is a ready-to-use, 2X end-point PCR master mix designed for simultaneous amplification of multiple targets in a single tube.

Supplier: FINNZYMES REAGENTS

Description: Phusion® Flash High-Fidelity PCR Master Mix is a 2X master mix enabling the use of extremely short PCR protocols without compromising either the fidelity or the yield in the reaction. The master mix utilises Phusion® Flash II DNA Polymerase, a proofreading DNA polymerase modified from Phusion® Hot Start II High-Fidelity DNA Polymerase. The fidelity of the Phusion® Flash II DNA Polymerase is 25-fold greater than <i>Taq</i>.

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Supplier: FINNZYMES REAGENTS

Description: Direct PCR Kits save time and cost by allowing amplification of DNA directly from the source material. Direct PCR is based on Thermo Scientific Phire® Hot Start II DNA Polymerase and Phusion® Hot Start II High-Fidelity DNA Polymerase. These fusion enzymes are exceptionally fast, robust and highly tolerant of many PCR inhibitors present in unpurified sample material. This allows reliable amplification in challenging conditions where conventional DNA polymerases are completely inhibited.

Supplier: FINNZYMES REAGENTS

Description: Phire® Hot Start II DNA Polymerase is faster, extremely robust, and capable of amplifying long DNA fragments with high yields. Phire® Hot Start II DNA Polymerase incorporates a dsDNA-binding domain which allows short extension times (10 to 15 s/kb), improves yields, and increases fidelity 2-fold compared to <i>Taq</i> DNA polymerase. In addition, the Hot Start technology allows complete reactivation of the enzyme in “zero-time” at standard cycling temperatures. This combination of features makes the polymerase an ideal solution for routine and high throughput PCR applications. Phire® Hot Start II DNA Polymerase delivers superior performance in conventional thermal cyclers as well as in fast instruments, such as the Piko® Thermal Cycler.

Supplier: FINNZYMES REAGENTS

Description: Phire® Hot Start II DNA Polymerase is faster, extremely robust, and capable of amplifying long DNA fragments with high yields. Phire® Hot Start II DNA Polymerase incorporates a dsDNA-binding domain which allows short extension times (10 to 15 s/kb), improves yields, and increases fidelity 2-fold compared to <i>Taq</i> DNA polymerase. In addition, the Hot Start technology allows complete reactivation of the enzyme in “zero-time” at standard cycling temperatures. This combination of features makes the polymerase an ideal solution for routine and high throughput PCR applications. Phire® Hot Start II DNA Polymerase delivers superior performance in conventional thermal cyclers as well as in fast instruments, such as the Piko® Thermal Cycler.

Supplier: FINNZYMES REAGENTS

Description: Phusion® Blood Direct PCR Master Mix is designed for amplification of DNA from whole blood. It eliminates the need for a separate DNA purification step prior to PCR. The modified Phusion® Hot Start II High-Fidelity DNA Polymerase is resistant to main PCR inhibitors present in blood and retains its activity at blood concentrations of up to 40% in the PCR reaction.

Supplier: FINNZYMES REAGENTS

Description: Phusion® U multiplex PCR master mix is a ready-to-use, 2X end-point PCR master mix designed for simultaneous amplification of multiple targets in a single tube.