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PerfeCTa® MultiPlex qPCR ToughMix®

Supplier: Quantabio
Description: PerfeCTa® MultiPlex qPCR ToughMix® is a 5X concentrated, ready to use reaction cocktail for Real-Time quantitative PCR (qPCR) that overcomes many known inhibitors of PCR often present in crude samples extracted from environmental specimens, plant tissues, or animal tissues. It contains all components, except primers, probes and templates. The 5X concentrated ToughMix® allows addition of higher amounts of template and improved detection sensitivity with low concentration samples. PerfeCta® MultiPlex qPCR ToughMix® has been optimised to deliver maximum PCR efficiency, sensitivity, and specificity in reduced reaction volumes with fast cycle or conventional PCR cycling protocols.

MSDS Certificates

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PerfeCTa® qPCR FastMix® II

Supplier: Quantabio
Description: PerfeCTa® qPCR FastMix® II is an advanced qPCR reagent system for both fast and conventional PCR cycling protocols or instruments. It is a versatile and robust solution that provides the ultimate sensitivity and high PCR efficiency using a variety of fluorogenic probe chemistries, including TaqMan® hydrolysis probes. PerfeCTa® qPCR FastMix® II is provided as a 2X concentrated ready to use reaction cocktail that contains all required reaction components, except primers, probe(s), and DNA template. The light blue colour of the AccuVue™ tracer dye simplifies reaction assembly in white, or clear, plates and helps to minimise pipetting or mixing errors. It does not interfere with qPCR performance or affect the stability of the product.

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PerfeCTa® qPCR FastMix™ reaction mixes

Supplier: Quantabio
Description: FastMixes include buffers and stabilisers optimised for maximum PCR efficiency, reproducibility, and sensitivity with TaqMan® and TaqMan® MGB probe chemistries. A key component of PerfeCTa® qPCR FastMixes is AccuFast™ <i>Taq</i> DNA polymerase that enables instant activation and rapid polymerisation kinetics. Upon heat activation (20 seconds at 95 °C), the antibodies irreversibly denature releasing fully active, unmodified <i>Taq</i> DNA polymerase. This promotes specific and efficient primer extension with the convenience of room temperature reaction assembly. FastMixes deliver maximum PCR efficiency, sensitivity, specificity, and a robust fluorescent signal with either fast or conventional cycling conditions.

MSDS

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PerfeCTa® qPCR ToughMix®

Supplier: Quantabio
Description: PerfeCTa® qPCR ToughMix® is a 2X concentrated ready to use reaction cocktail for PCR amplification of DNA templates that overcomes many known inhibitors of PCR often present in crude samples extracted from environmental specimens, plant tissues or animal tissues. It is a versatile and robust Real-Time qPCR reagent that provides maximum sensitivity and PCR efficiency with a variety of fluorogenic probe chemistries, including TaqMan® hydrolysis probes. PerfeCTa® qPCR ToughMix® contains all required reaction components, except primers, probe(s) and DNA template. The light blue colour of the AccuVue™ tracer dye simplifies reaction assembly in white, or clear plates and helps to minimise pipetting or mixing errors. It does not interfere with qPCR performance or affect the stability of the product.

MSDS Certificates

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qPCR SuperMix, PerfeCTa® MultiPlex

Supplier: Quantabio
Description: This SuperMix is a 2X concentrated, ready to use reaction cocktail for Real-Time qPCR that contains all components, except primers, probes, and templates. The system transcends multiplex limitations of conventional PCR master mixes, enabling unbiased amplification of up to five target sequences in a single tube. Suppression of low copy amplicons by high copy reference targets in the amplification is a common problem in multiplex PCR. This can skew, or mask the apparent representation and quantification of low copy target sequences. PerfeCTa® MultiPlex qPCR SuperMix delivers dynamic range and sensitivity to multiplexed qPCR that is comparable to that for singleplex qPCR probe assays without the need for limiting or variable primer concentrations.

MSDS Certificates

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Cyanine 5-dUTP

Catalog Number: (ENZOENZ42502)
Supplier: ENZO LIFE SCIENCES
Description: Cyanine 5-dUTP can replace TTP in reactions in which it serves as a substrate for E. coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase. Fluorescently labeled probes can be prepared with this fluorescent nucleotide by a variety of methods including nick translation, cDNA labeling and 3’-end labeling. Probes generated by these methods are suitable for use for the identification of specific sequences by in situ hybridization procedures on fixed cells and tissues by direct fluorescence detection. Cyanine 5-dUTP can also be used for multicolor fluorescence labeling.
UOM: 1 * 25 Nmo

New Product


Product Image-ENZOENZ42501

Cyanine 3-dUTP

Catalog Number: (ENZOENZ42501)
Supplier: ENZO LIFE SCIENCES
Description: Cyanine 3-dUTP can replace TTP in reactions in which it serves as a substrate for E. coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase. Fluorescently labeled probes can be prepared with this fluorescent nucleotide by a variety of methods including nick translation, cDNA labeling and 3’-end labeling. Probes generated by these methods are suitable for use for the identification of specific sequences by in situ hybridization procedures on fixed cells and tissues by direct fluorescence detection. Cyanine 3-dUTP can also be used for multicolor fluorescence labeling.
UOM: 1 * 25 Nmo

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Anti-STYK1 Mouse Monoclonal Antibody

Catalog Number: (PRSI32-221)
Supplier: ProSci Inc.
Description: Protein kinases (PKs) represent a well studied but most diverse protein superfamily. The covalent, reversible linkage of phosphate to serine, threonine, and tyrosine residues of substrate proteins by protein kinases is probably ubiquitous cellular mechanism for regulation of physiological processes. It is known to us that most signaling pathways impinge at some point on protein kinases. Here we report a human putative receptor protein kinase cDNA STYK1. The STYK1 cDNA is 2749 base pairs in length and contains an open reading frame encoding 422 amino acids. The STYK1 gene is mapped to human chromosome 12p13 and 11 exons were found. RT-PCR showed that STYK1 is widely expressed in human tissues.
UOM: 1 * 100 µl
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Real Time PCR Detection Test Kits, Supreme HLVd/LCV/CanCv

Supplier: BIOPREMIER
Description: This Supreme RT-PCR detection kit HLVd/LCV/CanCV is designed for the detection of Hop Latent Viroid (HLVd), Lettuce Chlorotic Virus (LCV) and Cannabis Cryptic Virus (CanCV) in a single PCR reaction (TETRAPLEX).

New Product

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Real Time PCR Detection Test Kits, Supreme HLVd (Hop Latent Viroid)

Supplier: BIOPREMIER
Description: SUPREME RT-PCR detection kit HLVd is designed for the detection of Hop Latent Viroid (HLVd) in a single PCR reaction. The kit is suitable for use in real time instruments with FAM and Cy5 detection channels.

New Product

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Verso™ qRT-PCR Kits

Supplier: Thermo Fisher Scientific
Description: The Verso™ qRT-PCR system combines the Verso™ RT enzyme with ABsolute™ Blue qPCR master mix to provide maximum convenience and versatility. Available in a 1- or 2-step format, as well as multiple pack sizes, to suit all application and throughput requirements.

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illustra™ PCR nucleotide mix dNTP set

Supplier: Cytiva
Description: PCR nucleotide mix dNTP set (2 mM, 10 mM, or 25 mM each A,C,G,T) for amplification, dideoxy sequencing, labelling, mutagenesis, cDNA synthesis, and expression profiling.

Product Image-27-9558-01

PCR beads, illustra™ PuReTaq Ready-To-Go™

Supplier: Cytiva
Description: PuReTaq RTG PCR beads and reagents ensures reliable and robust performance in both end point and RT-PCR amplifications and ensures the lowest possible levels of contaminating prokaryotic and eukaryotic nucleic acids.

MSDS

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dNTP mix

Catalog Number: (733-0548)
Supplier: Thermo Fisher Scientific
Description: A combined mixture of  dATP, dCTP, dGTP and dTTP containing 5 mM of each dNTP. Applications include PCR, RT-PCR, DNA labelling, and site-directed mutagenesis.
UOM: 1 * 1 mL
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TAQuest™ qPCR Master Mix with Helixyte™ Green *High ROX*

Supplier: AAT BIOQUEST
Description: TAQuest™ qPCR Master mix with Helixyte™ green is a ready-to-use 2X solution optimised for qPCR and two-step RT-qPCR.

New Product

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TAQuest™ qPCR Master Mix with Helixyte™ Green *No ROX*

Supplier: AAT BIOQUEST
Description: TAQuest™ qPCR Master mix with Helixyte™ green is a ready-to-use 2X solution optimised for qPCR and two-step RT-qPCR.

New Product

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on +353 1 88 22222.
Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on +353 1 88 22222
This product is marked as restricted and can only be purchased by approved Shipping Accounts. If you need further assistance, email VWR Regulatory Department at eurega_services@eu.vwr.com
-Additional Documentation May be needed to purchase this item. A VWR representative will contact you if needed.
This product has been blocked by your organisation. Please contact your purchasing department for more information.
The original product is no longer available. The replacement shown is available.
Product(s) marked with this symbol are discontinued - sold till end of stock. Alternatives may be available by searching with the VWR Catalog Number listed above. If you need further assistance, please call VWR Customer Service on +353 1 8822222.
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