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Antibody Fab and F(ab)₂ fragmentation of mouse IgG₁ kits

Supplier: G-Biosciences
Description: Antibody Fab and F(ab)₂ Fragmentation of Mouse IgG<sub>1</sub> Kits are designed for the generation and isolation of Fab and F(ab)₂ fragments from mouse IgG₁ molecules. The kit utilises Immobilised Ficin resin. Ficin (or Ficain) (~25000 Da) is a cysteine protease enzyme (EC 3.4.22.3) isolated from fig latex that has the endopeptidase activity to cleave immunoglobulin G molecules in the hinge region. Ficin has an effective range of pH 4 to 9,5 with an optimal pH of 6,5 and cleaves bonds that involve uncharged or aromatic amino acids. Ficin is typically used to cleave mouse IgG₁ as this is difficult to cleave with papain and pepsin. In the presence of 1 to 4 mM or 10 to 20 mM cysteine, ficin generates F(ab)₂ and Fab fragments respectively.

Image Unavailable-ENZOENZPRT1010500

AMPIGENE® HS Taq DNA Polymerase

Catalog Number: (ENZOENZPRT1010500)
Supplier: ENZO LIFE SCIENCES
Description: Increased sensitivity standard PCR for a broader range of samples, with enhanced speed, yield, and specificity.
UOM: 1 * 1 KIT
Product Image-733-2587

AccuStart™ II PCR Genotyping Kit

Supplier: Quantabio
Description: The AccuStart™ II Mouse Genotyping kit is designed for fast and easy preparation of PCR-ready DNA extracts and endpoint PCR analysis from tissues such as tail snips and ear punches commonly used for genotyping knockout and transgenic animals. The kit combines Extracta™ DNA Prep for PCR - Tissue with AccuStart™ II GelTrack™ PCR SuperMix in a single kit.

MSDS Certificates

Product Image-BSENBEK-2221-1P

Neurotrophin 3 (NT3) Rapid™ ELISA kit

Supplier: Biosensis
Description: The Biosensis NT3 Rapid™ enzyme-linked immune-sorbent assay (ELISA) kit is a sandwich ELISA that allows the specific, fast and reliable quantification of NT3 in less than 4 hours in cell culture supernatants and human plasma (EDTA and citrate) only if used as directed.

Product Image-BSENBEK-2220-1P

Nerve growth factor Receptor (NGFR/P75ECD) Rapid™ ELISA kit: Mouse

Supplier: Biosensis
Description: The Biosensis NGFR/p75ECD Rapid™ enzyme-linked immunosorbent assay (ELISA) kit is a sandwich ELISA that allows the quantification of the extracellular domain (ECD) of mouse p75 neurotrophin receptor in less than 4 hours in cell culture supernatants and urine only if used as directed.

Product Image-733-2546

VWR®, Red Taq DNA polymerase master mix

Supplier: VWR Chemicals
Description: VWR® Red <i>Taq</i> DNA Polymerase Master Mix, which also contains an inert red dye, can be directly loaded onto an agarose gel without addition of electrophoresis loading buffers.

Image Unavailable-ENZOBMLAK5560001

Fluor-de-Lys® SIRT2 fluorometric drug discovery assay kit

Catalog Number: (ENZOBMLAK5560001)
Supplier: ENZO LIFE SCIENCES
Description: A Fluor-de-Lys® fluorescent assay system. The SIRT2 Fluorescent Activity Assay/Drug Discovery Kit is a complete assay system designed to measure the lysyl deacetylase activity of the recombinant human SIRT2 included in the kit. The kit is ideal for chemical library screening for candidate inhibitors or activators or kinetic assay of the enzyme under varying conditions.
UOM: 1 * 1 KIT
Image Unavailable-ENZOENZNUC1011000

AMPIGENE® HS Taq Mix

Supplier: ENZO LIFE SCIENCES
Description: Increased sensitivity standard PCR mix for a broader range of samples, with enhanced speed, yield, and specificity.

Product Image-BSENBEK-2222-2P

Glial cell line-derived neurotrophic factor (GDNF) Rapid™ ELISA kit: human

Supplier: Biosensis
Description: The Biosensis GDNF Rapid™ enzyme-linked immunosorbent assay (ELISA) kit is a sandwich ELISA that allows the quantification of GDNF in less than 4 hours in cell culture supernatants, cell lysates, and guinea pig serum only if used as directed.

Product Image-BSENBEK-2230-2P

Glial cell line-derived neurotrophic factor (GDNF) Rapid™ ELISA kit: rat

Supplier: Biosensis
Description: The Biosensis GDNF RapidTM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of GDNF in less than 4 hours in cell culture supernatants, cell lysates, and serum only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application, in particular rat serum samples.

Image Unavailable-ENZOALX850247KI01

ApoSENSOR™ Cell viability assay kit

Catalog Number: (ENZOALX850247KI01)
Supplier: ENZO LIFE SCIENCES
Description: The assay utilizes bioluminescent detection of the ATP level for a rapid screening of apoptosis and cell proliferation simultaneously in mammalian cells. The assay utilizes the enzyme luciferase to catalyze the formation of light from ATP and luciferin. The light can be measured using a luminometer or Beta Counter. The assay can be fully automatic for high throughput (10 seconds/sample) and is highly sensitive (detects 10-100 mammalian cells/well).
UOM: 1 * 200 Tests
Product Image-EL0016

T4 DNA ligase

Catalog Number: (EL0016)
Supplier: Thermo Fisher Scientific
Description: This T4 DNA ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA. The enzyme repairs single-strand nicks in duplex DNA, RNA, or DNA/RNA hybrids. It also joins DNA fragments with either cohesive or blunt termini, but has no activity on single-stranded nucleic acids.
UOM: 1 * 1 KIT

Certificates


Image Unavailable-AGLS600357

Cloned Pfu DNA polymerase alternative detergents

Supplier: AGILENT
Description: Cloned Pfu DNA Polymerase for high fidelity and difficult, GC-Rich PCR.

Product Image-BSENBEK-2237-1P

proBDNF Rapid ELISA Kit: Human, Mouse, Rat (1 Plate)

Catalog Number: (BSENBEK-2237-1P)
Supplier: Biosensis
Description: The Biosensis proBDNF (Brain-derived neurotrophic factor, BDNF, Abrineurin) RapidTM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the specific, fast and reliable quantification of proBDNF in less than 4 hours in cell culture supernatants, human serum and EDTA-plasma only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application, in particular human blood samples.
UOM: 1 * 1 KIT
Product Image-TNB-6611-KIT

APO-BRDU™ kit, TUNEL Assay

Catalog Number: (TNB-6611-KIT)
Supplier: Tonbo Biosciences
Description: The APO-DIRECT™ reagent kit provides a two color TUNEL (Terminal deoxynucleotide transferase dUTP Nick End Labelling) assay for labeling DNA breaks and total cellular DNA to detect apoptotic cells by flow cytometry. The kit contains the instructions and reagents required for measuring apoptosis in cells including: positive and negative control cells for assessing reagent performance; washing, reaction and rinsing buffers for processing individual steps in the assay; terminal deoxynucleotidyl tranferase enzyme (TdT), fluorescein isothiocyanate (FITC) deoxyuridine triphosphate (FITC-dUTP) for labeling DNA breaks and PI/RNase A solution for counter staining the total DNA.
UOM: 1 * 50 Tests
Image Unavailable-AGLS600191

Pfu DNA ligase

Catalog Number: (AGLS600191)
Supplier: AGILENT
Description: <i>Pfu</i> DNA ligase is a recombinant enzyme derived from <i>Pyrococcus furiosus</i> hyperthermophilic marine archaebacterium that catalyses linkage of adjacent 5'-phosphate and 3'-hydroxy ends of double-stranded DNA at 45 to 80 °C, with a temperature optimum near 70 °C for nick-sealing reactions.
UOM: 1 * 1 items
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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on +353 1 88 22222.
Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on +353 1 88 22222
This product is marked as restricted and can only be purchased by approved Shipping Accounts. If you need further assistance, email VWR Regulatory Department at eurega_services@eu.vwr.com
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The original product is no longer available. The replacement shown is available.
Product(s) marked with this symbol are discontinued - sold till end of stock. Alternatives may be available by searching with the VWR Catalog Number listed above. If you need further assistance, please call VWR Customer Service on +353 1 8822222.
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