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RevertAid™ Reverse Transcriptase

Supplier: Thermo Fisher Scientific
Description: RevertAid™ Reverse Transcriptase (RT) is a genetically modified M-MuLV RT. It differs from the M-MuLV RT by its structure and catalytic properties. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity and a RNase H activity specific to RNA in RNA-DNA hybrids, which is significantly lower than that of Avian Myeloblastosis Virus (AMV) reverse transcriptase.

Product Image-EP0452

RevertAid™ H Minus Reverse Transcriptase

Supplier: Thermo Fisher Scientific
Description: RevertAid™ H Minus Reverse Transcriptase (RT) is a genetically modified M-MuLV RT. It differs from the M-MuLV RT by its structure and catalytic properties. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity, but lacks RNase H activity due to point mutation in the RNase H domain. RevertAid™ H Minus Reverse Transcriptase does not degrade RNA in RNA-DNA hybrids during synthesis of the first strand cDNA and therefore high yields of full-length cDNA from long templates are obtained. RevertAid™ H Minus Reverse Transcriptase maintains activity over a wide temperature range (42 to 55 °C), which makes the enzyme an ideal tool for reverse transcription of RNAs having a high degree of secondary structure.

Image Unavailable-USBIC2448-03E

Anti-KIT Rat Monoclonal Antibody (APC (Allophycocyanin)) [clone: 2B8]

Catalog Number: (USBIC2448-03E)
Supplier: US Biological
Description: Anti-KIT Rat Monoclonal Antibody (APC (Allophycocyanin)) [clone: 2B8]
UOM: 1 * 100 µG
Product Image-HAKATSCIR35

Circulating water baths, Precision™

Catalog Number: (HAKATSCIR35)
Supplier: THERMO ELECTRON LED
Description: These circulating water baths are ideal when temperature uniformity and control are particularly critical, such as when working with enzymes and in serological applications.
UOM: 1 * 1 items
Product Image-733-1175

cDNA synthesis kits, qScript™

Supplier: Quantabio
Description: qScript™ cDNA synthesis kit contains qScript™ Reverse Transcriptase, a mixture of a MMLV reverse transcriptase derivative and ribonuclease inhibitor protein, optimised for sensitive and reliable cDNA synthesis over a broad dynamic range of input RNA. cDNA synthesis reagents are provided in a variety of SuperMix and kit formats to suit specific applications.

MSDS Certificates

Product Image-460-0283

Digital block heater, compact

Supplier: Thermo Fisher Scientific
Description: These versatile digital block heaters provide good temperature uniformity and stability ensuring reproducible results. Units are compact, light weight with digital temperature controls and display, they maximises valuable bench space. The units are ideal for sample preparation, enzyme preservation, DNA amplification and enzyme–substrate reactions.

Product Image-460-0309

Digital block heater, with touch screen

Supplier: Thermo Fisher Scientific
Description: Designed to provide precise temperature stability and uniformity. The units have an easy to use touch screen control which displays time and temperature and are durable with a powder-coated steel body. Ideal for sample preparation, enzyme preservation, enzyme-substrate reactions, DNA amplification, electrophoresis gel degeneration and serum coagulation. Choice of models to hold 1, 2 and 4 interchangeable modular blocks to accommodate a variety of vessels and applications. Interchangeable aluminum alloy sample blocks designed to provide versatility, easy cleaning and disinfecting.

Product Image-460-0308

Digital block heaters

Supplier: Thermo Fisher Scientific
Description: Versatile digital block heaters offer a wide range of blocks to accommodate various tubes provide temperature uniformity and stability for reproducible results. The units have digital controls and display of time and temperature and are durable with a powder-coated steel body. Ideal for sample preparation, enzyme preservation, enzyme-substrate reactions, DNA amplification, electrophoresis gel degeneration and serum coagulation. Choice of models to hold 1, 2 and 4 interchangeable modular blocks to accommodate a variety of vessels and applications.

Product Image-BTRABR1401502

Rapid translation system (RTS), insect membrane kits

Supplier: biotechrabbit
Description: RTS 100 Insect Membrane Kit provides high yields of <i>in vitro</i> synthesised post-translationally modified eukaryotic proteins.

Image Unavailable-71978-3

One step RT-PCR master mix kit

Catalog Number: (71978-3)
Supplier: Merck Millipore (Novagen)
Description: The One Step RT-PCR Master Mix Kit allows rapid, sensitive analysis of gene expression from tissues and cells. It can replace methods for detecting and quantifying gene expression such as Northern blots,<i> in situ</i> hybridisation, dot blots, S nuclease assays and conventional two step RT-PCR. The kit utilises recombinant <i>Thermus thermophilus</i> (rTth) DNA Polymerase, which acts as both a thermostable RNA dependent DNA polymerase and a DNA dependent DNA polymerase. The rTth DNA Polymerase is provided in a 2X master mix with an antibody for antibody-mediated hot start, optimised buffer, and ultrapure deoxynucleotides. Antibody-mediated Hot Start enhances specificity of both reverse transcription and PCR. The kit enables cDNA synthesis from input RNA followed by PCR amplification of the cDNA in a single reaction, with no additional hands-on requirement for buffer changes or adding reagents. Typically, detection of a specific transcript requires only 2 hours.
UOM: 1 * 1 KIT
Product Image-PRSI26-027

Anti-POMT1 Rabbit Polyclonal Antibody

Catalog Number: (PRSI26-027)
Supplier: ProSci Inc.
Description: POMT1 is an O-mannosyltransferase that requires interaction with the product of the POMT2 gene for enzymatic function. The encoded protein is found in the membrane of the endoplasmic reticulum. Defects in this gene are a cause of Walker-Warburg syndrome (WWS) and limb-girdle muscular dystrophy type 2K (LGMD2K).O-mannosylation is an important protein modification in eukaryotes that is initiated by an evolutionarily conserved family of protein O-mannosyltransferases. POMT1 shares sequence similarity with protein O-mannosyltransferases of S. cerevisiae. In yeast, these enzymes are located in the endoplasmic reticulum (ER) and are required for cell integrity and cell wall rigidity. POMT1 also shows similarity to the Drosophila 'rotated abdomen' (rt) gene, which when mutated causes defects in myogenesis and muscle structure.
UOM: 1 * 50 µG
Product Image-1.50036.1003

HPLC columns, Purospher® STAR RP-18 endcapped

Supplier: Merck
Description: Fast method development of complex samples across the pH range from 1,5 to 10,5 with different mobile phases and temperature conditions.
Purospher® STAR RP-18 endcapped HPLC columns are designed for universal use. Basic, neutral and metal-chelating compounds can easily be separated with simple mobile phases – naturally without peak tailing. Thanks to its outstanding performance and stability, Purospher® STAR RP-18 endcapped offers maximum flexibility in method development. Robust methods can be developed over the entire pH range from 1,5 to 10,5. High pH-stability allows the separation of strongly basic compounds with alkaline eluents. The combination of high purity silica, best all-round retention characteristics, outstanding pH stability up to pH 10,5, and suitability for up to 100% aqueous mobile phases, make Purospher® STAR RP-18 endcapped an all-round top performance column, almost universal in its range of applications.

MSDS

Product Image-BTRABR1402401

Cell-free protein synthesis, rapid translation system (RTS) linear template kits

Supplier: biotechrabbit
Description: Expression kits for PCR-based generation of linear templates.

Product Image-K1621

RevertAid™ First strand cDNA synthesis kit

Supplier: Thermo Fisher Scientific
Description: The RevertAid™ First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates. The kit is suitable for synthesis of cDNA up to 13 kb. The kit uses RevertAid™ Reverse Transcriptase which has lower RNase H activity compared to AMV reverse transcriptase. The recombinant RiboLock™ RNase Inhibitor, supplied with the kit, effectively protects RNA template from degradation. It is fully compatible with reverse transcription reaction, as it maintains activity at temperatures up to 55 °C.

Product Image-733-2340

Rapid translation system (RTS), E. coli disulfide and fab kits

Supplier: biotechrabbit
Description: The Rapid Translation System (RTS) is a scalable<i> in vitro</i> protein expression platform that produces large amounts of protein for characterisation studies, functional assays, or structural analysis. RTS enables both transcription and translation utilising High-Yield (HY) and continuous-exchange, cell-free (CECF) technologies, with systems based on bacterial (<i>E. coli</i>) or eukaryotic (wheat germ) cell lysates. RTS offers the advantages of a cell-free protein expression system, whilst eliminating the need for laborious up and downstream steps typically associated with cell-based expression systems.

Image Unavailable-R6950-01

Bacterial RNA kit, E.Z.N.A.®

Supplier: OMEGA BIO-TEK
Description: The E.Z.N.A.® Bacterial RNA kit is designed for isolation of high-quality total RNA from variety of bacterial strains. Up to 1×10⁹ log-phase bacterial cells can be processed. This kit uses an improved lysis procedure to ensure the complete lysis of bacterial cells. Purified RNA is suitable for downstream applications such as RT-PCR and hybridisation techniques.

MSDS Certificates

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on +353 1 88 22222
This product is marked as restricted and can only be purchased by approved Shipping Accounts. If you need further assistance, email VWR Regulatory Department at eurega_services@eu.vwr.com
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