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Urea ≥98%, white powder for molecular biology

Supplier: MP Biomedicals
Description: Applications:
Urea is used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies and in the extraction of the mitochondrial complex. It is commonly used to solubilize and denature proteins for denaturing isoelectric focusing and two-dimensional electrophoresis and in acetic acid-urea PAGE gels. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form. Urea is used in cell or tissue culture media to increase the osmolality. Urea has also been used as fertilizer because of the easy availability of nitrogen; in animal feeds; it is reacted with aldehydes to make resins and plastics; condensed with malonic ester to form barbituric acid; used in the paper industry to soften cellulose.
Biochem/physiol Actions:
Urea has been shown to act as an aldosterone antagonist in the development of peanut agglutinin binding in cultured embryonic renal collecting duct epithelial cells. Urea is the principal end product of nitrogen metabolism in most mammals, formed by the enzymatic reactions of the Kreb's cycle.
Typical Working Concentration:
The use of 2 g/L urea in the culture of Kluyveromyces marxianus to produce a thermostable extracellular lipase has been described. Urea is typically used at a concentration of 8 M for protein denaturation or solubilization. A final concentration of 5 M urea is commonly used in molecular biology for sequencing gels. To prevent carbamylation, do not heat urea containing buffers above 37 °C

Product Image-9731-15

Ammonia solution 28,0 - 30,0 % (NH₃ basis), CMOS for microelectronic, J.T.Baker®

Supplier: Avantor
Description: Ammonia solution 28,0 - 30,0 % (NH₃ basis), CMOS for microelectronic, J.T.Baker®

MSDS Certificates

Image Unavailable-8.04608.1000

Hydrazinium hydroxide ≥99% for synthesis

Supplier: Merck
Description: Hydrazinium hydroxide ≥99% for synthesis

MSDS Certificates

Image Unavailable-51788-5ML-F

Aniline, Supelco®

Supplier: Merck
Description: Aniline, Supelco®

Certificates

Image Unavailable-H62305.03

Boc-Glu-NH₂ 98%

Supplier: Thermo Fisher Scientific
Description: Boc-Glu-NH₂ 98%

Image Unavailable-45456-250MG

Diphenylamine, Supelco®

Catalog Number: (45456-250MG)
Supplier: Merck
Description: Diphenylamine, Supelco®
UOM: 1 * 250 mg

Certificates


Image Unavailable-APOSBIPG1410-500MG

Fmoc-NH-PEG4-CH2CO2H

Catalog Number: (APOSBIPG1410-500MG)
Supplier: Apollo Scientific
Description: Fmoc-NH-PEG4-CH2CO2H
UOM: 1 * 500 mg
Image Unavailable-8.04604.9055

Hydrazine hydrate ≥80% in aqueous solution for synthesis, Sigma-Aldrich®

Supplier: Merck
Description: Hydrazine hydrate ≥80% in aqueous solution for synthesis, Sigma-Aldrich®

Certificates

Product Image-9721-15

Ammonia solution 28,0 - 30,0% (NH₃ basis), BAKER ANALYZED® ACS, J.T.Baker®

Supplier: Avantor
Description: Ammonia solution 28,0 - 30,0% (NH₃ basis), BAKER ANALYZED® ACS, J.T.Baker®

Certificates

Product Image-1.32227.0100

Ammonium CRMs, standard solutions, Supelco®

Supplier: Merck
Description: NH₄Cl in H₂O

MSDS Certificates

Image Unavailable-1.04220.5000

Guanidinium hydrochloride, Millipore®

Supplier: Merck
Description: Guanidinium hydrochloride, Millipore®

MSDS

Image Unavailable-1.01164.0250

Ammonium fluoride, EMSURE® ACS for analysis, Supelco®

Supplier: Merck
Description: Ammonium fluoride, EMSURE® ACS for analysis, Supelco®

MSDS

Image Unavailable-0834-26

Guanidinium hydrochloride, GenAR® for biotechnology, Macron Fine Chemicals™

Catalog Number: (0834-26)
Supplier: Avantor
Description: Guanidinium hydrochloride, GenAR® for biotechnology, Macron Fine Chemicals™
UOM: 1 * 200 L
Image Unavailable-APOSBIPG1413-500MG

Fmoc-NH-PEG8-CH2COOH

Catalog Number: (APOSBIPG1413-500MG)
Supplier: Apollo Scientific
Description: Fmoc-NH-PEG8-CH2COOH
UOM: 1 * 500 mg
Product Image-7716-88

Guanidinium hydrochloride ≥99.5%, GenAR® for biotechnology, Macron Fine Chemicals™

Supplier: MACRON AVANTOR BRAND
Description: Tested for absence of detectable aldehydes and ammeline. DNase-, RNase-, and protease-free.

Certificates

Image Unavailable-APOSBIPG1481-100MG

Mal-amido-PEG12-NHS

Supplier: Apollo Scientific
Description: Mal-amido-PEG12-NHS

Inquire for Price
Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on +353 1 88 22222.
Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on +353 1 88 22222
This product is marked as restricted and can only be purchased by approved Shipping Accounts. If you need further assistance, email VWR Regulatory Department at eurega_services@eu.vwr.com
-Additional Documentation May be needed to purchase this item. A VWR representative will contact you if needed.
This product has been blocked by your organisation. Please contact your purchasing department for more information.
The original product is no longer available. The replacement shown is available.
Product(s) marked with this symbol are discontinued - sold till end of stock. Alternatives may be available by searching with the VWR Catalog Number listed above. If you need further assistance, please call VWR Customer Service on +353 1 8822222.
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