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Luminol and oxidising solutions

Supplier: Thermo Fisher Scientific
Description: For chemiluminecent HRP-based detection of western blotting systems. It is soluble in most polar organic solvents but is insoluble in water.

MSDS Certificates

Product Image-PIER31490

Horseradish peroxidase, Pierce™

Catalog Number: (PIER31490)
Supplier: Thermo Fisher Scientific
Description: Pierce™ Horseradish peroxidase (HRP) is purified horseradish peroxidase enzyme for use in activity assays and conjugation to antibodies for ELISA, Western blot and immunohistochemistry applications.
UOM: 1 * 10 mg
Product Image-PIER32110

Antibody extender solution NC, Miser™

Catalog Number: (PIER32110)
Supplier: Thermo Fisher Scientific
Description: Miser™ Antibody Extender Solution NC is a post-transfer nitrocellulose membrane-treatment reagent that enables Western blot detection with much less primary antibody.
UOM: 1 * 500 mL
Product Image-RPN2134

Amersham™ ECL™ Western Blotting Detection Reagent, Cytivia

Supplier: Cytiva
Description: Enhanced chemiluminescence (ECL) detection reagents for reliable detection of medium and high expression proteins.

Product Image-PIER21059

Restore™ Western Blot Stripping Buffer

Supplier: Thermo Fisher Scientific
Description: Although optimising assay conditions is the best way to achieve optimum results, re-performing the gel electrophoresis process to test each new primary antibody or antibody concentration is time-consuming and expensive. Restore™ Western blot stripping buffer offers an effective alternative.

Image Unavailable-786-423

High molecular weight transfer buffer (5X)

Catalog Number: (786-423)
Supplier: G-Biosciences
Description: High molecular weight transfer buffer (5X) is designed to facilitate the transfer of notoriously difficult high molecular weight proteins (>70 kDa) during Western blotting.
UOM: 1 * 1 L
Product Image-PIER15165

HRP conjugates, HisProbe™

Catalog Number: (PIER15165)
Supplier: Thermo Fisher Scientific
Description: HisProbe™-HRP is a nickel (Ni²⁺)-activated derivative of horseradish peroxidase (HRP) that enables direct, IMAC-based detection of His-tagged proteins and other histidine-rich proteins in Western blots and microplates.
UOM: 1 * 2 mg
Image Unavailable-A3417.5000

CheLuminate-HRP PicoDetect Chemiluminescent Detection Kit

Catalog Number: (A3417.5000)
Supplier: PanReac AppliChem
Description: The CheLuminate-HRP PicoDetect is a complete kit with ready to use reagents for chemiluminescent detection of immobilised proteins (Western blot) or immobilised nucleic acids (Southern or Northern blot), conjugated with horseradish peroxidase (HRP) directly or indirectly.
UOM: 1 * 1 KIT
Product Image-PIER34577

SuperSignal™ Pierce™ West Pico PLUS chemiluminescent substrate

Supplier: Thermo Fisher Scientific
Description: SuperSignal™ West Pico PLUS Chemiluminescent Substrate is an enhanced chemiluminescent (ECL) horseradish peroxidase (HRP) substrate that enables picogram- to high femtogram-level protein detection by Western blot analysis.

MSDS

Product Image-PIER34042

Substrate for western blotting, 1-Step™ NBT/BCIP

Catalog Number: (PIER34042)
Supplier: Thermo Fisher Scientific
Description: The 1-Step NBT/BCIP is a one-component sensitive precipitating alkaline phosphatase substrate for immunoblotting, immunohistochemistry, and <i>in situ</i> hybridisation applications.
UOM: 1 * 250 mL
Product Image-ROCKKCA004

Chemiluminescent western blot kits, FemtoMax™

Supplier: Rockland Immunochemicals
Description: These Chemiluminescent Western Blot Kits allow for the detection of primary polyclonal or monoclonal antibodies provided by the user. After protein separation and transfer, the membrane is probed with primary antibody. Detection of the membrane-bound primary antibody-antigen complex is achieved by the addition of a secondary antibody conjugated to the enzyme horseradish peroxidase. The enzyme reacts with a specialised formulation of luminol, an extremely sensitive, non radioactive substrate that emits light and allows visualisation using X-ray film or other imaging methods, including highly sensitive CCD cameras and imaging systems. Because of the extremely high sensitivity of the kit, primary and secondary antibodies can be used at greater dilutions. Primary antibodies currently diluted 1:1000 using an ECL™ substrate is the equivalent of a 1:5000 dilution using the FemtoMax™ substrate.

Product Image-RPN418

Western blotting blocking reagent, Amersham ECL™ Prime

Catalog Number: (RPN418)
Supplier: Cytiva
Description: Amersham ECL Prime Blocking Reagent is blocking Amersham Protran NC and Amersham Hybond PVDF membranes used with Amersham ECL start, Amersham ECL, Amersham, ECL Prime, Amersham ECL Select and Amersham ECL Plex.
UOM: 1 * 40 g

MSDS


Product Image-PIER15168

West HisProbe kit, SuperSignal™

Catalog Number: (PIER15168)
Supplier: Thermo Fisher Scientific
Description: SuperSignal™ West HisProbe Kit contains HisProbe-HRP conjugate and reagents required for detection of polyhistidine-tagged protein by Western blotting. HisProbe-HRP Conjugate is a nickel (Ni²⁺)-activated derivative of horseradish peroxidase (HRP) that enables direct, IMAC-based detection of His-tagged proteins.
UOM: 1 * 1 KIT
Product Image-732-3879

Automated western device, eZwest™ Lite

Catalog Number: (732-3879)
Supplier: Genscript
Description: eZwest™ Lite is an automated western blotting device providing an easy and simple solution in your western blotting workflow. After proteins are transferred onto the membrane, load the membrane into our eZwest™ Lite device for automated blocking, incubation, and washing cycles. The user only needs to prepare the required reagents, run the customised program, and eZwest™ Lite will take care of the rest. Once the program is finished, the user can collect the results and immediately proceed to detection and analysis steps. eZwest™ Lite is easy to use and has minimal footprint.
UOM: 1 * 1 SET
Product Image-PIER34070

Substrate for western blotting, 1-Step™ NBT/BCIP plus suppressor

Catalog Number: (PIER34070)
Supplier: Thermo Fisher Scientific
Description: The 1-Step NBT/BCIP plus suppressor is a one-component alkaline phosphatase substrate that inhibits endogenous phosphatase activity. This substrate reacts with calf intestinal alkaline phosphatase to produce a dark purple precipitate and is ideal for immunohistochemistry and <i>in situ</i> hybridisation applications.
UOM: 1 * 100 mL
Product Image-PIERA39260

Sulfo-SBED Biotin Label Transfer Kit and Reagents - Western Blot Application, Pierce™

Supplier: Thermo Fisher Scientific
Description: Sulfo-SBED is a trifunctional crosslinking reagent containing a biotin, a sulfonated N-hydroxysuccinimide (Sulfo-NHS) active ester and a photoactivatable aryl azide

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on +353 1 88 22222
This product is marked as restricted and can only be purchased by approved Shipping Accounts. If you need further assistance, email VWR Regulatory Department at eurega_services@eu.vwr.com
-Additional Documentation May be needed to purchase this item. A VWR representative will contact you if needed.
This product has been blocked by your organisation. Please contact your purchasing department for more information.
The original product is no longer available. The replacement shown is available.
Product(s) marked with this symbol are discontinued - sold till end of stock. Alternatives may be available by searching with the VWR Catalog Number listed above. If you need further assistance, please call VWR Customer Service on +353 1 8822222.
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