Supplier: Merck Millipore (Novagen)

Description: pET-41 series is designed for cloning and high-level expression of peptide sequences fused with the 220 aa GST•Tag™ protein. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase. The f1 origin is oriented so that infection with helper phage will produce virions containing single stranded DNA that corresponds to the coding strand.

Supplier: Cytiva

Description: Poly(A) (polyadenylic acid) can be used to evaluate the binding of potential therapeutic agents to mRNA in drug development research.

Certificates

Catalog Number: (69750-3)

Supplier: Merck Millipore (Novagen)

Description: The pET-24a-d(+) vectors carry an N-terminal T7-Tag® sequence plus an optional C-terminal His-Tag® sequence. These vectors differ from pET-21a-d(+) only by their selectable marker (kanamycin vs ampicillin resistance).

UOM: 1 * 10 µG

Sale

Catalog Number: (69660-3)

Supplier: Merck Millipore (Novagen)

Description: The pET-14b vector carries an N-terminal His·Tag® sequence followed by a thrombin cleavage site and three cloning sites.

UOM: 1 * 10 µG

Sale

Catalog Number: (69909-3)

Supplier: Merck Millipore (Novagen)

Description: The pET-30a-c(+) vectors carry an N-terminal His Tag®/thrombin/S Tag™/enterokinase configuration plus an optional C-terminal His tag sequence.

UOM: 1 * 10 µG

Sale

Catalog Number: (27-1524-01)

Supplier: Cytiva

Description: M13 phage for the GST expression system.

UOM: 1 * 100 µl

Sale

Catalog Number: (ORIGMG225346)

Supplier: OriGene

Description: Slc7a11 (GFP-tagged) - Mouse solute carrier family 7 (cationic amino acid transporter y+ system) member 11 (Slc7a11), (10 µg).

UOM: 1 * 10 µG

Sale

Catalog Number: (27-1542-01)

Supplier: Cytiva

Description: The <i>E. coli </i>BL21 strain is protease-deficient for optimal expression of a variety of recombinant proteins.

UOM: 1 * 1 Vial

Sale

Catalog Number: (13-6943-00)

Supplier: VWR Chemicals

Description: Plasmid Miniprep Kit I for the easy, quick and efficient extraction of highly pure plasmid DNA. Isolate up to 30 µg of high quality plasmid DNA from 1 to 5 ml bacterial cultures in 30 minutes or less. Plasmid DNA obtained with peqGOLD Plasmid Miniprep kits can directly be used for a wide range of downstream applications.

UOM: 1 * 5 Tests

MSDS Certificates Sale

Catalog Number: (AGLS400763)

Supplier: AGILENT

Description: The StrataPrep plasmid preparation kits provide a rapid method for purifying plasmid DNA from bacterial cultures.

UOM: 1 * 1 items

Sale

Supplier: OMEGA BIO-TEK

Description: E.Z.N.A.® Plasmid Midi Kit offers a novel and reliable method of isolating up to 200 µg of plasmid DNA in less than 60 minutes. This kit combines HiBind® membrane technology with the time-tested consistency of alkaline SDS lysis of bacterial cells to deliver high-quality plasmid DNA. The DNase/RNase-free HiBind® DNA midi columns operate by eliminating time consuming phenol-chloroform extractions and alcohol precipitations. Plasmid purification using the E.Z.N.A.® Plasmid Midi Kit follows a simple bind-wash-elute procedure thereby making it possible for multiple samples to be processed. Although yields vary according to plasmid copy number, <i>E.coli </i>strain, and conditions of growth, 50 ml of an overnight culture in LB medium will typically produce 200 µg of high-copy number plasmid DNA. When working with low-copy number plasmids the starting culture volume can be increased up to 100 ml. High-quality DNA is ready for immediate use in routine molecular biology applications, such as automated fluorescent sequencing, restriction enzyme digestion, and transfection screening.

MSDS

Supplier: OMEGA BIO-TEK

Description: Plasmid isolated with traditional purification procedures normally contain high levels of endotoxins that can significantly interfere with transfection experiments downstream. The E.Z.N.A.® Endo-Free Plasmid kits integrate an efficient endotoxin removal step into the plasmid purification procedure to produce high quality transfection grade plasmid.

MSDS Certificates

Supplier: OMEGA BIO-TEK

Description: The E.Z.N.A.® Plasmid Maxi Kit is the maxi scale system to deliver high-quality plasmid DNA using the spin column format, without costly accessories. The HiBind® DNA Maxi column facilitates the binding, washing, and elution steps, thus enabling multiple samples to be simultaneously processed. Although yields vary according to plasmid copy number, <i>E.coli</i> strain, and conditions of growth, 50 to 200 ml of overnight culture in LB medium typically produces 0,5 to 1 mg of high copy number plasmid DNA. Up to 500 ml of culture may be processed when working with low copy number plasmid. The product is suitable for automated fluorescent DNA sequencing (typical reads exceed 800 bp), restriction enzyme digestion, transfection of mammalian cells, transcription<i> invitro</i>, and other applications.

MSDS Certificates

Catalog Number: (D6943-03)

Supplier: OMEGA BIO-TEK

Description: Isolate up to 25 µg plasmid DNA from 1 to 5 ml bacterial cultures using mini spin columns.

UOM: 1 * 1 KIT

Sale

Catalog Number: (D6942-03)

Supplier: OMEGA BIO-TEK

Description: Isolate up to 25 µg plasmid DNA from 1 to 5 ml bacterial cultures using mini spin columns.

UOM: 1 * 1 KIT

Sale

Supplier: OMEGA BIO-TEK

Description: E.Z.N.A.® Fastfilter® Plasmid kits rapidly purify plasmid DNA utilising lysate clearance filter syringes, whilst E-Z 96® Fastfilter Plasmid kits employ lysate clearance plates in a 96-well format. Fastfilter® kits allow midi- and maxi-scale plasmid isolation in less than 40 m.