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IPG buffers

Supplier: Cytiva
Description: IPG buffers are ampholyte-containing buffer concentrates specifically formulated for use with Immobiline™ DryStrip gels for isoelectric focusing, run as the first dimension of 2-D electrophoresis or as a separate application. Using Immobiline DryStrip gels together with matching IPG buffer gives high sample loading capacity, improves resolution, and increases the probability of detecting low abundance proteins.

Image Unavailable-BOSSBS-0889R

Anti-HSP90AA1 Rabbit Polyclonal Antibody

Catalog Number: (BOSSBS-0889R)
Supplier: Bioss
Description: In mammalian cells there are at least two Hsp90 isoforms, Hsp90a and Hsp90b which are encoded by separate genes. These ubiquitous and highly conserved proteins account for 1-2% of all cellular proteins in most cells. Hsp90 is part of the cells powerful network of chaperones to fight the deleterious consequences of protein unfolding caused by nonphysiological conditions. However, in the absence of stress, Hsp90 is a necessary component of fundamental cellular processes such as hormone signaling and cell cycle control. In this context several key regulatory proteins such as steriod receptors, cell cycle kinases involved in signal transduction and p53 have been identified as substrates of Hsp90. It has been suggested that Hsp90 acts as a capacitor for morphological evolution by buffering widespread variation, which may affect morphogenic pathways.
UOM: 1 * 100 µl
Product Image-LONZ50655

DNA gel loading buffer 6X

Catalog Number: (LONZ50655)
Supplier: LONZA
Description: This buffer is a Ficoll based loading buffer containing Ficoll®, EDTA, xylene cyanol, and bromophenol blue. Ideal for separation of DNA fragments with improved resolution.
UOM: 1 * 5 items

MSDS


Product Image-700-0354

VWR® shiroGEL vertical 20, Vertical Electrophoresis Systems

Supplier: VWR Collection
Description: The shiroGEL vertical 20 offers application flexibility and is distinguished by its ease of gel casting and set-up, requiring only four screws to secure up to four 20×20 cm gels. This vertical screw clamp technology distributes pressure evenly along the height of gel rather than in the centre to eliminate plate bowing and gel compression, while maintaining a leakproof seal during casting. A built-in inner buffer chamber within the PAGE insert allows set up to be completed without inclusion of an upper buffer chamber. These systems are ideal for 1D and 2D SDS-PAGE, native, preparative, gradient and high resolution nucleic acid electrophoresis.

Image Unavailable-ENZOADISPA845D

Anti-HSP91 Rat Monoclonal Antibody

Catalog Number: (ENZOADISPA845D)
Supplier: ENZO LIFE SCIENCES
Description: The 90kDa molecular chaperone family includes 90 kDa heat shock protein Hsp90 and 94 kDa glucose-regulated protein grp94, both major molecular chaperones of the cytosol and the endoplasmic reticulum. Mammalian cells contain isoforms Hsp90α and Hsp90β, encoded by separate genes. The amino acid sequences of human and yeast Hsp90-alpha are 85% and 90% homologous to those of Hsp90β , respectively. All known members of the Hsp90 protein family are highly conserved, especially in the N-terminal and C-terminal regions containing independent chaperone sites with different substrate specificity. These ubiquitous and highly conserved proteins account for 1-2% of all cellular proteins in most cells. Hsp90 functions as part of the cell’s powerful network of chaperones to fight the deleterious consequences of protein unfolding caused by non-physiological conditions. In the absence of stress, however, Hsp90 provides a necessary component of such fundamental cellular processes as hormone signaling and cell cycle control. In this context, researchers identified key regulatory proteins as substrates of Hsp90, including steroid receptors, cell cycle kinases involved in signal transduction, and p53. Hsp90 may act as a capacitor for morphological evolution by buffering widespread variation, potentially affecting morphogenic pathways. When temperature and other stress factors compromise Drosophila Hsp90 buffering, cryptic variant expression occurs, and selection can lead to the continued expression of these traits even after Hsp90 function is restored.
UOM: 1 * 50 µl
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Tricine buffer substance

Supplier: G-Biosciences
Description: This tricine is a buffer component used for the separation of low molecular weight peptides.

Product Image-ENZOADISPA845F

Anti-HSP91 Rat Monoclonal Antibody

Catalog Number: (ENZOADISPA845F)
Supplier: ENZO LIFE SCIENCES
Description: The 90 kDa molecular chaperone family includes 90 kDa heat shock protein Hsp90 and 94 kDa glucose-regulated protein grp94, both major molecular chaperones of the cytosol and the endoplasmic reticulum. Mammalian cells contain isoforms Hsp90α and Hsp90β, encoded by separate genes. The amino acid sequences of human and yeast Hsp90-alpha are 85% and 90% homologous to those of Hsp90β , respectively. All known members of the Hsp90 protein family are highly conserved, especially in the N-terminal and C-terminal regions containing independent chaperone sites with different substrate specificity. These ubiquitous and highly conserved proteins account for 1 to 2% of all cellular proteins in most cells. Hsp90 functions as part of the cell’s powerful network of chaperones to fight the deleterious consequences of protein unfolding caused by non-physiological conditions. In the absence of stress, however, Hsp90 provides a necessary component of such fundamental cellular processes as hormone signaling and cell cycle control. In this context, researchers identified key regulatory proteins as substrates of Hsp90, including steroid receptors, cell cycle kinases involved in signal transduction, and p53. Hsp90 may act as a capacitor for morphological evolution by buffering widespread variation, potentially affecting morphogenic pathways. When temperature and other stress factors compromise Drosophila Hsp90 buffering, cryptic variant expression occurs, and selection can lead to the continued expression of these traits even after Hsp90 function is restored.
UOM: 1 * 200 µl
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DNA Ladders in TE buffer

Supplier: Biotium
Description: DNA ladders in TE buffer are used for sizing linear double-stranded DNA fragments separated by gel electrophoresis. The 1 kb DNA ladder is suitable for sizing DNA fragments from 250 bp to 10 kb, while the 100 bp DNA ladder is suitable for sizing DNA fragments from 100 bp to 1500 bp.

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Product Image-700-0292

VWR® shiroGEL Modular mini vertical PAGE systems

Catalog Number: (700-0292)
Supplier: VWR Collection
Description: The shiroGEL mini vertical PAGE system is modular, allowing PAGE and electroblotting to be carried out in the same tank, simply by changing the inserts. The system features inserts for each application, and a common buffer tank in which to run them. Each insert has its own electrode assembly, which connects to the lid of the buffer tank. For safety, the power leads connect directly to the lid. When the lid is removed, power is disconnected from the system. The inserts also have a small pad that fits into position in the lid. This ensures that the lid is always properly placed and aids in its removal. The buffer tank is moulded to prevent separation and leaking. A variety of spacers, combs and other accessories are available to customise the system. All spacers and combs are colour coded or clearly labelled to indicate thickness.
UOM: 1 * 1 items
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HEPPSO (β-hydroxy-4-(2-hydroxyethyl)piperazine-1-propanesulphonic acid) ≥99%, white powder

Supplier: MP Biomedicals
Description: Used as a buffer in biological research. HEPPSO is an efficient separator in protein resolution by IEF.

MSDS

Product Image-78833.

Nuclear and cytoplasmic extraction reagents, NE-PER

Supplier: Thermo Fisher Scientific
Description: The NE-PER Nuclear and Cytoplasmic Extraction Kit provides for efficient cell lysis and extraction of separate cytoplasmic and nuclear protein fractions in less than two hours. The nuclear protein extraction method involves simple, stepwise lysis of cells and centrifugal isolation of nuclear and cytoplasmic protein fractions. A benchtop microcentrifuge, tubes and pipettors are the only tools required. The NE-PER Reagents efficiently solubilise and separate cytoplasmic and nuclear proteins into fractions with minimal cross-contamination or interference from genomic DNA and mRNA. The isolated proteins can be used to perform immunoassays as well as protein interaction studies such as mobility shift assays (EMSA), co-immunoprecipitation (Co-IP) and pull-down assays.

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Disposable columns, DISPOSAFLEX®, KIMBLE®

Catalog Number: (SCERSP420160-0000)
Supplier: DWK Life Sciences
Description: This is a multi purpose column designed to satisfy a wide range of analytical separations using aqueous buffers and typically used for the extraction of the cyclic nucleotides cAMP and cGMP from sample preparations using AG1-X8.
UOM: 1 * 100 items
Product Image-GENSM00654

Bis-Tris mini protein gels

Supplier: Genscript
Description: GenScript's Bis-Tris gels are high-performing polyacrylamide gels designed to separate a wide range of protein sizes by electrophoresis. The gels are cast in a neutral pH buffer that minimises polyacrylamide hydrolysis and increases gel stability. Furthermore, the neutral pH which minimises protein modification when compared to Tris-glycine gels.

Product Image-PIER43230

Desalting columns, Pierce™

Supplier: Thermo Fisher Scientific
Description: These desalting columns are ready-to-use, disposable, gel filtration columns for separating proteins and other macromolecules from low molecular weight buffer salts and reagents.

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CEP coated capillaries

Catalog Number: (HEWLG1600-62318)
Supplier: Agilent
Description: CEP coated capillaries contain a permanently bonded polymer coating. This CEP coating shields the silanol functionality of the capillary surface and helps prevent sample adsorption. Additionally, EOF is nearly eliminated, making the capillary ideal for applications such as DNA separations with sieving polymer buffers.
UOM: 1 * 2 items
Product Image-BINZ113178

High purity hardened qualitative filter paper

Supplier: Ahlstrom-Munksjö
Description: Ahlstrom-Munksjö’s qualitative grades of filter paper are recommended for use in analytical methods which determine or identify particulate constituents of a mixture irrespective of the amount present. Qualitative filter papers are often used in routine separation work that still requires high purity and consistent performance. These filter papers covers a wide range of laboratory applications, as liquids clarification, qualitative analytical separations for precipitates and buffers filtration, and are also used for soil analysis and in food and beverage testing. Wet-strengthened grades contain a reinforcement agent that increases their resistance to rupture when wet, such as in vacuum filtration, and their resistance when filtering acidic solutions. High Purity hardened filter papers grades 1288 to 1291 are also produced from cellulose with an alpha content of nearly 100% resulting in an ash content of <0,1%.

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on +353 1 88 22222
This product is marked as restricted and can only be purchased by approved Shipping Accounts. If you need further assistance, email VWR Regulatory Department at eurega_services@eu.vwr.com
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This product has been blocked by your organisation. Please contact your purchasing department for more information.
The original product is no longer available. The replacement shown is available.
Product(s) marked with this symbol are discontinued - sold till end of stock. Alternatives may be available by searching with the VWR Catalog Number listed above. If you need further assistance, please call VWR Customer Service on +353 1 8822222.
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