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Product Image-25-6400-80

illustra™ TempliPhi Large construct DNA amplification kit

Catalog Number: (25-6400-80)
Supplier: Cytiva
Description: illustra™ TempliPhi large construct DNA amplification kit (TempliPhi LC kit) is used to prepare templates for BAC or fosmid DNA sequencing.
UOM: 1 * 1.000 Tests
Image Unavailable-AGLSDNF-935-K1000

Qualitative DNA Kits

Supplier: AGILENT
Description: DNA fragment analysis for PCR, genotyping, and microsatellite applications.

Product Image-PRSI56-993

Anti-HTRA3 Rabbit Polyclonal Antibody

Catalog Number: (PRSI56-993)
Supplier: ProSci Inc.
Description: Insulin-like growth factors (IGFs) stimulate the proliferation and differentiation of a vast number of cell types. The action of the growth factors is mediated and controlled by a complex system of components, including several proteases that cleave the IGF-Binding Proteins. HtrA1 is a 480 aa protein that contains an N-terminus homologous to MAC25 (IGFBP7) with a conserved Kazal-type serine protease inhibitor motif, as well as a C-terminal PDZ domain. Semiquantitative RT-PCR and immunoblot analyses showed an approximately 7-fold increase of PRSS11 in osteoarthritis cartilage compared with controls. HTRA2 is released from mitochondria and inhibits the function of XIAP by direct binding in a way similar to SMAC. Moreover, when overexpressed extramitochondrially, HTRA2 induced atypical cell death, which was neither accompanied by a significant increase in caspase activity nor inhibited by caspase inhibitors, including XIAP. A catalytically inactive mutant of HTRA2, however, did not induce cell death. Suzuki et al. (2001) concluded that HTRA2 is a SMAC-like inhibitor of IAP (inhibitor of apoptosis proteins) activity with a serine protease-dependent cell death-inducing activity.
UOM: 1 * 400 µl

New Product


Product Image-13-6492-02

Peqlab peqGOLD Cycle-Pure™, PCR Clean-up, Kits

Supplier: VWR Chemicals
Description: Purification of single- or double-stranded DNA from PCR or other enzymatic reactions.

MSDS Certificates

Image Unavailable-EP0282

Taq DNA polymerase (native, without BSA)

Catalog Number: (EP0282)
Supplier: Thermo Fisher Scientific
Description: <i>Taq</i> DNA Polymerase is a highly thermostable DNA polymerase from the thermophilic bacterium <i>Thermus aquaticus</i>. The enzyme catalyses 5'→3' synthesis of DNA, has no detectable 3'→5' exonuclease (proofreading) activity and possesses low 5'→3' exonuclease activity. In addition, <i>Taq</i> DNA polymerase exhibits deoxynucleotidyl transferase activity, which frequently results in the addition of extra adenines at the 3’-end of PCR products. Native <i>Taq</i> DNA polymerase is preferred for amplification of bacterial DNA sequences homologous to those found in <i>E.coli</i>.
UOM: 1 * 1 KIT
Product Image-786-817

Pfu DNA polymerase

Supplier: G-Biosciences
Description: <i>Pfu</i> DNA Polymerase, derived from the hyperthermophilic archae <i>Pyrococcus furiosus</i>, has superior thermostability and proofreading properties compared to other thermostable polymerases. It has a molecular weight of 90 kDa and can amplify DNA targets up to 2 kb. The elongation velocity is 0,2~0,4 kb/min (70~75 °C). <i>Pfu</i> DNA Polymerase possesses 3' to 5' exonuclease proofreading activity that enables the polymerase to correct nucleotide misincorporation errors. This means that<i> Pfu</i> DNA Polymerase-generated PCR fragments will have fewer errors than <i>Taq</i>-generated PCR inserts. Using <i>Pfu</i> DNA Polymerase results in blunt-ended PCR products, which are ideal for cloning into blunt-ended vectors. <i>Pfu</i> DNA Polymerase is superior for techniques that require high-fidelity DNA synthesis.

Product Image-28-9035-29

illustra™ TempliPhi™ sequence resolver kit

Supplier: Cytiva
Description: For the amplification of difficult templates for successful DNA sequencing in 18 hours.

Image Unavailable-786-443

dNTP mix

Supplier: G-Biosciences
Description: Aqueous mixture of dATP, dCTP, dGTP and dTTP each at a final concentration of 10 or 25 mM.

Image Unavailable-AATB17223

3'-O-Azidomethyl-dCTP

Supplier: AAT BIOQUEST
Description: 3'-O-Azidomethyl-dCTP is one of the four essential dNTPs (A, C, G, T) that can be used for the DNA sequencing by synthesis (SBS).

New Product

Product Image-732-4818

Workstation and stacking system for PCR tubes and plates

Catalog Number: (732-4818)
Supplier: Thermo Fisher Scientific
Description: Convenient platform for tube handling and reagent dispensing before and after sample processing.
UOM: 1 * 10 items
Product Image-786-449

Taq DNA polymerase

Supplier: G-Biosciences
Description: <i>Taq</i> DNA Polymerase is a highly thermostable recombinant DNA polymerase derived from the thermophile <i>Thermus aquaticus</i>. The molecular weight of the recombinant protein is 94 kD. The <i>Taq</i> Polymerase is able to amplify DNA up to 5 kb with an elongation velocity of 0,9 to 1,2 kb/min at 70 to 75 °C. The error rate of this <i>Taq</i> Polymerase is ~2,2×10⁻⁵ nucleotide⁻¹ cycle⁻¹. <i>Taq</i> DNA polymerase catalyses the 5’→3’ synthesis of DNA. The enzyme has no detectable 3’→5’ proofreading exonuclease activity, and possesses low 5’→3’ exonuclease activity, which results in a 3’-dA overhang on the PCR product

Product Image-M04018BF

VWR®, DNA extraction Kit for Real-time PCR food samples analysis

Catalog Number: (M04018BF)
Supplier: VWR Chemicals
Description: Rapid method for DNA extraction and purification from all types of microorganisms in a variety of matrices for molecular biology.
UOM: 1 * 1 KIT

Certificates


Product Image-F-122S

Phire® Hot Start II DNA polymerase

Supplier: FINNZYMES REAGENTS
Description: Phire® Hot Start II DNA Polymerase is faster, extremely robust, and capable of amplifying long DNA fragments with high yields. Phire® Hot Start II DNA Polymerase incorporates a dsDNA-binding domain which allows short extension times (10 to 15 s/kb), improves yields, and increases fidelity 2-fold compared to <i>Taq</i> DNA polymerase. In addition, the Hot Start technology allows complete reactivation of the enzyme in “zero-time” at standard cycling temperatures. This combination of features makes the polymerase an ideal solution for routine and high throughput PCR applications. Phire® Hot Start II DNA Polymerase delivers superior performance in conventional thermal cyclers as well as in fast instruments, such as the Piko® Thermal Cycler.

Image Unavailable-AATB17026-100NM

Cyanine-5- dUTP [Cy5-dUTP] *1 mM in TE Buffer (pH 7,5)*

Supplier: AAT BIOQUEST
Description: This Cy5-dUTP conjugate can be used as a red fluorescence colour with SpectrumFRed™ filter set (SpectrumFRed™ is the trademark of Vysis).

New Product

Product Image-AATB17036

Fluorescein-12-dATP *1 mM in Tris Buffer (pH 7,5)*

Catalog Number: (AATB17036)
Supplier: AAT BIOQUEST
Description: Fluorescein-12-dATP might be incorporated into a DNA sequence as its natural counterpart dATP to incorporate a fluorescein tag in a DNA molecule.
UOM: 1 * 25 Nmo

New Product


Product Image-AATB17044

iFluor® 488-dUTP *1 mM in TE Buffer (pH 7,5)*

Catalog Number: (AATB17044)
Supplier: AAT BIOQUEST
Description: This iFluor 488-dUTP conjugate is used as a green fluorescence colour with SpectrumGreen™ filter set (SpectrumGreen™ is the trademark of Vysis).
UOM: 1 * 100 Nmo

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on +353 1 88 22222
This product is marked as restricted and can only be purchased by approved Shipping Accounts. If you need further assistance, email VWR Regulatory Department at eurega_services@eu.vwr.com
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The original product is no longer available. The replacement shown is available.
Product(s) marked with this symbol are discontinued - sold till end of stock. Alternatives may be available by searching with the VWR Catalog Number listed above. If you need further assistance, please call VWR Customer Service on +353 1 8822222.
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