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Benzonase® Nuclease

Description: Benzonase® nuclease degrades all forms of DNA and RNA while having no proteolytic activity. Benzonase® is compatible with BugBuster® and PopCulture® protein extraction reagents and therefore can be added to these reagents to eliminate viscosity and remove nucleic acids from <i>E. coli </i>extracts.
Catalog Number: 70746-3
UOM: 1 * 10 KU
Supplier: Merck Millipore (Novagen)
Product Image-786-181

Protein extraction kit, tissue PE LB™

Description: Tissue PE LB™ has been developed for extraction of total biologically active, soluble proteins from animal tissues. Tissue PE LB™ is based on an organic buffer and utilises a mild non-ionic detergent, chelating agent, and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. Depending on the required downstream application, additional agents such as reducing agents and protease inhibitors may be added into Tissue PE LB™. Tissue PE LB™ has been tested for use with a wide variety of fresh and frozen animal tissues.
Catalog Number: 786-181
UOM: 1 * 500 mL
Supplier: G-Biosciences
Product Image-786-411

Protein extraction kit, Insect PE LB™

Description: Insect PE LB™ has been developed for extraction of total biologically active, soluble proteins from adherent or suspension cultured insect cells, including Sf9 and Sf21. Insect PE LB™ utilises a mild non-ionic detergent and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. The Insect PE LB™ is fully compatible with downstream processes, such as electrophoresis and chromatography. Depending on the required downstream application, additional agents such as reducing agents and protease inhibitors may be added into Insect PE LB™.
Catalog Number: 786-411
UOM: 1 * 250 mL
Supplier: G-Biosciences
Product Image-PIER78870

Pierce™ DNA Extraction Reagent Kit, Yeast

Description: The Pierce™ yeast DNA extraction reagent kit uses Y-PER yeast protein extraction reagent to quickly extract and purify genomic and plasmid DNA from yeast.
Catalog Number: PIER78870
UOM: 1 * 1 KIT
Supplier: Thermo Fisher Scientific
Product Image-786-180

Protein extraction kit, mammalian cell PE LB™

Description: Mammalian Cell PE LB™ has been developed for extraction of total biologically active, soluble proteins from mammalian cultured cells. The Mammalian Cell PE LB™ is based on organic buffering agents and utilises a mild non-ionic detergent, chelating agent, and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. Depending on the required downstream application, additional agents such as reducing agents, phosphatase and protease inhibitors may be added into Mammalian Cell PE LB™. Mammalian Cell PE LB™ has been tested on a wide variety of mammalian cells and can be used for both suspension and adherent cells.
Catalog Number: 786-180
UOM: 1 * 500 mL
Supplier: G-Biosciences
Product Image-PIER20395

Immobilised jacalin agarose, Pierce™

Description: Jacalin is an alpha-D-galactose binding lectin extracted from jack-fruit seeds (Artocarpus integrifolia). This lectin is a glycoprotein (approx. 40 kDa) composed of four identical subunits. Jacalin binds to human IgA1 (also called serum or secretory IgA) with high specificity (i.e., it does not bind IgG or IgM). Thus, Jacalin that has been immobilised on crosslinked beaded agarose is useful for purifying human IgA from serum or colostrum samples.
Catalog Number: PIER20395
UOM: 1 * 5 mL
Supplier: Thermo Fisher Scientific

MSDS


Product Image-PIER89839

Organelle Enrichment Kits

Description: Organelle enrichment kit for lysosomes enables enrichment of intact organelles from cells and tissue. The kit provides the media and reagents necessary to accomplish subcellular fractionation using high-speed density gradient centrifugation.
Catalog Number: PIER89839
UOM: 1 * 1 KIT
Supplier: Thermo Fisher Scientific
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Lysonase

Description: Lysonase™ bioprocessing reagent is an optimised, ready-to-use convenient blend of rLysozyme™ solution and benzonase nuclease which is ideal for protein extraction.
Catalog Number: 71230-3
UOM: 1 * 0,2 mL
Supplier: Merck Millipore (Novagen)

MSDS


Product Image-786-316

Cell surface protein isolation and labelling kit, HOOK™

Description: The HOOK™ Cell Surface Protein Isolation Kit uses HOOK™ biotin labelling and purification technology in conjunction with Mammalian Cell PE LB™ Lysis Buffer to conveniently label cell surface proteins and isolate them for further analysis, including Western blotting. The Kit is supplied with all the necessary reagents and buffers for convenience and improved reproducibility. The kit is compatible with a wide variety of mammalian cells and can be used to compare treated and untreated cells and differences between different cell lines.
Catalog Number: 786-316
UOM: 1 * 1 KIT
Supplier: G-Biosciences
Product Image-AATB24100

ReadiUse™ Bacterial Cell Lysis Buffer *5X* (10 ml)

Description: Cell lysis refers to the breaking down of cells, and it is often used to analyze specific cellular compositions such as proteins, lipids, nucleic acids, reporter molecules, cell signal molecules and other small biomolecules.
Catalog Number: AATB24100
UOM: 1 * 10 mL
Supplier: AAT BIOQUEST
Product Image-TB110

Kingdom blot

Description: Kingdom blot is prepared using total protein extracted from a variety of species, where whole species is not available the whole liver lysate is used. These blots can be used to identify similar proteins in other species, including human, chicken, frog, worm, Drosophila, yeast, Aspergillus, <i>E. coli</i>, Rhizobium and green algae.
Catalog Number: TB110
UOM: 1 * 1 items
Supplier: G-Biosciences
Product Image-733-2877

VWR®, Fast extract DNA solution

Description: The fast extract DNA solution provides rapid and efficient extraction of PCR-ready DNA from mammalian and plant tissues. DNA is ready in eight minutes. The one-reagent protocol is divided into two simple heating steps, which can be directly followed by PCR analysis, such as screening and genotyping.
Catalog Number: 733-2877
UOM: 1 * 500 Tests
Supplier: VWR Chemicals
Product Image-PIER75705

β-Galactosidase Assay Kit and Reagents

Description: The Mammalian β-Galactosidase Assay Reagent Kit is a simple and versatile assay to measure β-galactosidase activity in cultured mammalian cells. Cells grown on a plate may be lysed and tested for β-galactosidase activity by adding a single all-in-one lysis-and-assay solution. Alternatively, M-PER™ Mammalian Protein Extraction Reagent may be used to prepare lysate from washed adherent or pelleted cells, and then a portion of the lysate used to assay for β-galactosidase activity. With M-PER Reagent, there is no need to scrape cells or to perform freeze-thaw cycles. The remaining lysates prepared with M-PER™ Reagent may be used for applications such as reporter assays (luciferase, β-galactosidase and chloramphenical acetyltransferase), protein kinase assays (PKA, PKC, and tyrosine kinase), immunoassays (Western blot, ELISA, RIA) and protein purification.
Catalog Number: PIER75705
UOM: 1 * 25 mL
Supplier: Thermo Fisher Scientific
Image Unavailable-ENZOADI801526

Extraction reagents

Description: Concentrated lysis buffer for preparation of cell and tissue extracts.
Catalog Number: ENZOADI801526
UOM: 1 * 10 mL
Supplier: ENZO LIFE SCIENCES

New Product


Product Image-733-2879

VWR® Fast extract genotyping PCR kit

Description: This fast extract genotyping PCR kit consists of a fast extraction DNA solution and VWR® Red Taq DNA polymerase 2X master mix. It is ideal for genotyping of DNA extracted from mammalian tissues, plant material, saliva or bacteria, providing PCR-ready DNA in 8 minutes and genotyping results in less than 1,5 hours.
Catalog Number: 733-2879
UOM: 1 * 500 Tests
Supplier: VWR Chemicals
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Phenol, white solid, Ultrapure

Description: Phenol is useful for extraction of viral, plasmid and phage DNAs, and to solubilise and dissociate proteins. It is used as a general disinfectant, either in solution or mixed with slaked lime, etc., for toilets, stables, cesspools, floors, drains, etc.; for the manufacture of colorless or light-colored artificial resins, many medical and industrial organic compounds and dyes; as a reagent in chemical analysis. Aqueous solutions are used as topical anesthetic; topical antiseptic; topical antipruritic. Phenol is used in DNA extractions for removing protein.
Catalog Number: ICNA04818048
UOM: 1 * 500 g
Supplier: MP Biomedicals
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