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Image Unavailable-BOSSBS-8539R-HRP

Anti-TLR5 Rabbit Polyclonal Antibody (HRP (Horseradish Peroxidase))

Catalog Number: (BOSSBS-8539R-HRP)
Supplier: Bioss
Description: Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.
UOM: 1 * 100 µl
Image Unavailable-BOSSBS-8539R-A647

Anti-TLR5 Rabbit Polyclonal Antibody (Alexa Fluor® 647)

Catalog Number: (BOSSBS-8539R-A647)
Supplier: Bioss
Description: Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.
UOM: 1 * 100 µl

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Product Image-71009-4

Protein extraction reagents, CytoBuster™

Supplier: Merck Millipore (Novagen)
Description: The CytoBuster™ protein extraction reagent is a proprietary formulation of detergents optimised for efficient extraction of soluble proteins from mammalian and insect cells. The gentle, non-ionic composition of the reagent enables isolation of functionally active endogenous or expressed proteins without a need for secondary treatment such as sonication or freeze/thaw.

Product Image-PIER87793

Accessories for primary cell isolation kits, Pierce™

Supplier: Thermo Fisher Scientific
Description: Neuronal Protein Extraction Reagent (N-PER), sufficient reagent to extract protein from ~10 g of cells or tissue, Pierce

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PopCulture® reagent

Supplier: Merck Millipore (Novagen)
Description: PopCulture® Reagent is a detergent-based concentrate that can be added directly to cultures of <i>E. coli</i> to effectively extract proteins without the need for cell harvest. Recombinant proteins can be directly screened in the crude extract, or purified by adding an affinity matrix, washing the matrix-target protein complex to remove spent culture medium and cellular contaminants, and eluting the purified protein from the matrix. The PopCulture protein purification procedure is ideally suited to high-throughput robotic processing of samples for proteomics research or any application that would benefit from the increased speed and convenience.

Product Image-PIER78510

T-PER™ Tissue Protein Extraction Reagent

Catalog Number: (PIER78510)
Supplier: Thermo Fisher Scientific
Description: Tissue Protein Extraction Reagent (T-PER ) enables mild extraction of total protein from tissue samples.
UOM: 1 * 500 mL

MSDS


Product Image-PIER78999

Y-PER™ plus Yeast Protein Extraction Reagent, Dialysable

Catalog Number: (PIER78999)
Supplier: Thermo Fisher Scientific
Description: This extraction reagent is formulated to effectively lyse yeast and bacterial cells to extract and solubilise proteins for analysis.
UOM: 1 * 500 mL
Product Image-REST25849

QuEChERS extraction salts, Q-sep®

Catalog Number: (REST25849)
Supplier: Restek
Description: QuEChERS methods are fast, easy, and cost-effective, and Restek Q-sep® products make QuEChERS procedures even simpler.
UOM: 1 * 50 items
Product Image-PIER89802

I-PER™ Insect Cell Protein Extraction Reagents

Catalog Number: (PIER89802)
Supplier: Thermo Fisher Scientific
Description: The I-PER Insect Cell Protein Extraction Reagent enables gentle and effective extraction of soluble protein from baculovirus-infected insect cells grown in suspension or monolayer culture. The baculovirus insect cell expression system is an efficient and popular system for production of recombinant (eukaryotic) proteins in cell culture. Proteins expressed in baculoviral systems can be used for structural analyses, biochemical assays and a variety of other applications. I-PER Reagent maintains functionality of extracted proteins and is directly compatible with downstream applications such as protein assays, Western blotting and 6xHis-tagged protein purification.
UOM: 1 * 250 mL
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Master mix, BugBuster®

Supplier: Merck Millipore (Novagen)
Description: BugBuster® master mix combines BugBuster® protein extraction reagent with Benzonase® Nuclease and rLysozyme™ solution in one convenient reagent. It allows for maximum recovery of active soluble protein from both Gram-negative and Gram-positive bacteria. The master mix provides sufficient reagents for protein extraction from 20 g and 100 g cell paste.

Product Image-PIER90402

Mass tagging, mass tag labelling reagents, AminoxyTMT™, Pierce™

Supplier: Thermo Fisher Scientific
Description: The carbonyl-reactive Thermo Scientific™ aminoxyTMT™ (Tandem Mass Tag™) Label Reagents enable multiplexed characterisation and quantitation of carbonyl-containing biomolecules (carbohydrates, steroids, oxidised proteins) by mass spectrometry (MS).

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Protein extraction reagent, BugBuster® (primary amine-free), Millipore®

Supplier: Merck Millipore (Novagen)
Description: BugBuster® (primary amine-free) Protein Extraction Reagent is a special formulation of BugBuster designed for applications where primary amines would interfere if present in the protein extract, such as protein immobilisation or cross-linking. The PIPPS buffer used in BugBuster® (primary amine-free) has a similar buffer capacity and pH range as the original Tris-buffered BugBuster®, but will not complex metal ions, also making it ideally suited for extraction of metal dependent proteins.

Product Image-PIER78505

Mammalian protein extract reagents, M-PER™

Supplier: Thermo Fisher Scientific
Description: The M-PER Mammalian Protein Extraction Reagent is designed to provide highly efficient total protein extraction from cultured mammalian cells.

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Protein extraction reagents, BugBuster® and BugBuster® plus Benzonase®, Millipore®

Supplier: Merck Millipore (Novagen)
Description: BugBuster® Protein Extraction reagent is formulated for the gentle disruption of the cell wall of <i>E. coli</i>, liberating soluble protein. It provides a simple, rapid, low-cost alternative to mechanical methods such as French Press or sonication for releasing expressed target protein in preparation for purification or other applications. The formulation utilises a detergent mix that is capable of cell wall perforation without denaturing soluble protein.

Product Image-786-428

Adenine deaminase

Catalog Number: (786-428)
Supplier: G-Biosciences
Description: Adenine deaminase converts adenine to hypoxanthine. It is a highly purified recombinant enzyme protein supplied at a 1 µM concentration.
UOM: 1 * 0,25 mL
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Protein extraction kits, BugBuster® Plus Lysonase™

Supplier: Merck Millipore (Novagen)
Description: BugBuster® Plus Lysonase™ includes BugBuster Protein Extraction Reagent and Lysonase Bioprocessing Reagent, an optimised, ready-to-use blend of rLysozyme™ and Benzonase® Nuclease in a single kit. This powerful combination of two reagents allows maximum recovery of active soluble protein from both Gram-negative and Gram-positive bacteria. The BugBuster® and rLysozyme™ efficiently lyse the bacterial cells while the Benzonase® degrades nucleic acids to facilitate downstream processing. Requires 5 ml BugBuster and 10 µl Lysonase per 1 g cell paste. The kits provide sufficient reagents for protein extraction from 20 g or 100 g cell paste.

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on +353 1 88 22222.
Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on +353 1 88 22222
This product is marked as restricted and can only be purchased by approved Shipping Accounts. If you need further assistance, email VWR Regulatory Department at eurega_services@eu.vwr.com
-Additional Documentation May be needed to purchase this item. A VWR representative will contact you if needed.
This product has been blocked by your organisation. Please contact your purchasing department for more information.
The original product is no longer available. The replacement shown is available.
Product(s) marked with this symbol are discontinued - sold till end of stock. Alternatives may be available by searching with the VWR Catalog Number listed above. If you need further assistance, please call VWR Customer Service on +353 1 8822222.
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