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Supplier: TriLink BioTechnologies
Description: NLS-Cre Recombinase mRNA is a capped and polyadenylated messenger RNA encoding Cre recombinase fused to a nuclear localisation sequence (NLS). Cre recombinase is a tyrosine recombinase that catalyses recombination between two loxP sites.

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Supplier: TriLink BioTechnologies
Description: <p><span style="color:black">This construct expresses an enhanced version of the green fluorescent protein (EGFP), originally isolated from the jellyfish <span style="color:black"><em>Aequorea victoria</em></span><span style="color:black">, as self-amplifying RNA (saRNA). EGFP is a commonly used direct detection reporter in mammalian cells, yielding bright green fluorescence with an emission peak at 509 nm.</span></span></p>

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Supplier: TriLink BioTechnologies
Description: <p>mCherry mRNA encodes the fluorescent protein, mCherry, which is derived from DsRed, a protein found in <em>Discosoma </em>sp. mCherry is a monomeric fluorophore with a peak absorption at 587 nm and emission at 610 nm. It is stable and resistant to photobleaching.</p>

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Supplier: TriLink BioTechnologies
Description: β-galactosidase (β-gal), a protein product of the bacterial LacZ gene, catalyzes the conversion of β-galactosides into monosaccharides. β-gal is a common marker gene used to assess transfection efficiency.

Supplier: TriLink BioTechnologies
Description: mCherry mRNA encodes the fluorescent protein, mCherry, which is derived from DsRed, a protein found in <em>Discosoma sp.</em> mCherry is a monomeric fluorophore with a peak absorption at 587 nm and emission at 610 nm. It is stable and resistant to photobleaching.

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Supplier: TriLink BioTechnologies
Description: EPO mRNA encodes the human erythropoietin (EPO) protein, a hormone that controls erythropoiesis, or red blood cell production. EPO also plays a role in wound healing and the brain’s response to neural injury. Kariko <em>et al.</em> showed that transfection of EPO mRNA <em>in vivo</em> resulted in significant increases of both reticulocyte counts and hematocrits.

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Supplier: TriLink BioTechnologies
Description: The EGFP mRNA expresses an enhanced version of the green fluorescent protein, originally isolated from the jellyfish, <em>Aequorea victoria</em>. EGFP is a commonly used direct detection reporter in mammalian cell culture, yielding bright green fluorescence with an emission peak at 509 nm.

Supplier: TriLink BioTechnologies
Description: Renilla luciferase (Renilla Luc) is a bioluminescent reporter protein derived from Renilla reniformis (sea pansy). In the presence of the cofactor, coelenterazine, Renilla luciferase produces light. Renilla Luc mRNA can be used as an internal control in combination with FLuc mRNA to test different conditions, such as dosage or chemical modifications, and their effect on translation.

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Supplier: TriLink BioTechnologies
Description: <p>EPO mRNA encodes the human erythropoietin (EPO) protein, a hormone that controls erythropoiesis, or red blood cell production. EPO also plays a role in wound healing and the brain’s response to neural injury. Kariko et al. showed that transfection of EPO mRNA <em>in vivo</em> resulted in significant increases of both reticulocyte counts and hematocrits.</p>

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Supplier: TriLink BioTechnologies
Description: NLS-Cre Recombinase mRNA is a capped and polyadenylated messenger RNA encoding Cre recombinase fused to a nuclear localisation sequence (NLS). Cre recombinase is a tyrosine recombinase that catalyzes recombination between two loxP sites.

Supplier: TriLink BioTechnologies
Description: <p><span style="color:black"> BspQI (isoschizomers: SapI, PciSI and LguI) is a Type IIS restriction enzyme known for its unique cleavage properties. It recognizes asymmetric DNA sequences, cleaves the double-stranded DNA outside of the recognition site, and generates a 5′ overhang.</span></p>

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Catalog Number: (SOLUK-7413-25)
Supplier: TriLink BioTechnologies
Description: <p><span style="color:black">TriLink’s CleanCap® AG (3′ OMe) CleanScript™ IVT kit provides essential components for mRNA synthesis using co-transcriptional capping by in vitro transcription (IVT). The kit components are sufficient to run 25 of 100 µl reactions following our recommended IVT protocol with co-transcriptional capping. It includes a cap analog, modified and unmodified nucleotides, IVT enzymes including CleanScribe RNA polymerase, 10X CleanScript IVT reaction buffer, and a linearized FLuc DNA control template.</span></p>
UOM: 1 * 100 µl

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Supplier: Polyplus-transfection
Description: FectoCHO® expression system consists in the synergistic association of a novel CHO chemically defined medium, FectoCHO® CD expression medium and a powerful transfection reagent, FectoPRO®.

Supplier: Polyplus-transfection
Description: PEIpro® is a ready to use 1 mg/ml optimised linear polyethylenimine (PEI) reagent for the production of virus and recombinant proteins by Transient Gene Expression (TGE) in suspension-adapted mammalian cell lines cultivated in shaker flasks, platform shakers or stirred tank bioreactors.
Supplier: G-Biosciences
Description: T4 UvsX DNA recombinase mediates DNA strand exchange between homologous chromosomes. The protein forms a right-handed nucleoprotein complex on single ssDNA called the presynaptic filament that can search for homology in duplex DNA and pair the recombining DNA molecules to form a DNA joint molecule. This process is aided by ssDNA binding proteins and recombination mediators. The filament is then elongated by DNA polymerase with the newly synthesised strand displacing the old strand. The newly synthesised duplex DNA can then act as a template for the next cycle to achieve exponential amplification of dsDNA. <i>E.coli</i> cells expressing recombinant T4 UvsX gene.

Supplier: Polyplus-transfection
Description: The FectoPRO® Transfection Kit is specifically designed for enhanced Transient Gene Expression (TGE) in suspension CHO and HEK-293 cells in various serum-free media, using low DNA amount (<1 μg/ml of cell culture).
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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on +353 1 88 22222
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