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Product Image-OZBIHYC03000

DNA transfection reagent, HYPE-CHO™

Supplier: OZ BIOSCIENCES
Description: HYPE-CHO™ transfection kit has been designed for large scale up transient transfection and high protein expression, such as antibodies. The system is optimised for maximum efficiency in all CHO-S cells.

Product Image-K1261

LIC cloning and expression kits, aLICator™, Fermentas

Supplier: Thermo Fisher Scientific
Description: The aLICator™ LIC Cloning and Expression System is designed for fast and efficient ligation independent cloning and tight regulation of gene expression in <i>E. coli</i>. The plate bacterial expression vectors are designed for high levels of target protein expression in concert with minimal basal (uninduced) expression, which permits expression of proteins that are toxic to <i>E. coli</i> cells.

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DNA kit, BacMagic™-2

Catalog Number: (72157-3)
Supplier: Merck Millipore (Novagen)
Description: The BacMagic™-2 DNA Kit provides a positive selection for creating baculoviruses recombinants in preparation for generating recombinant protein expression in insect cells. The kit improves on the traditional method for generating recombinant baculoviruses by eliminating the tedious, time-consuming steps of plaque purification.
UOM: 1 * 1 KIT
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Yeast transformation kit, Alkali-Cation™, MP Biomedicals

Catalog Number: (ICNA112200200)
Supplier: MP Biomedicals
Description: Alkali-Cation™ Yeast Transformation Kit provides an efficient method for yeast transformation and has been successfully used to transform linear DNA into <i>Pichia pastoris</i> for protein expression studies.
UOM: 1 * 250 Tests
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Transfection kit, BacMagic™-2

Catalog Number: (72158-3)
Supplier: Merck Millipore (Novagen)
Description: The Novagen® BacMagic™-2 transfection kit provides a positive selection for creating baculovirus recombinants in preparation for recombinant protein expression in insect cells. The kit improves on the traditional method for generating recombinant baculoviruses by eliminating the tedious, time-consuming steps of plaque purification.
UOM: 1 * 1 KIT
Product Image-OZBIFF51000

DNA transfection reagent, FlyFectIN™

Supplier: OZ BIOSCIENCES
Description: FlyFectIN™ transfection reagent is a powerful reagent based on Tee-technology and specifically designed to obtain highly efficient and reproducible transfection of insect cells. It is adapted to the delivery of all types of nucleic acids. It can be used for many applications including the production of recombinant protein using Baculovirus expression system.

Product Image-OZBIDG80500

DNA transfection reagent, DreamFect™ Gold

Supplier: OZ BIOSCIENCES
Description: DreamFect™ Gold transfection reagent allows transfecting all types of nucleic acids with a very high efficiency. Based on Tee-technology (Triggered Endosomal Escape), DreamFect™ Gold delivers a large quantity of nucleic acids leading to higher protein expression compared to other transfection reagents. It is fully and does not interfere with cellular mechanisms. Consequently, high cell viability is maintained in every experiment and any potential secondary effect is avoided.

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Transfection kit, BacMagic™

Catalog Number: (71546-3)
Supplier: Merck Millipore (Novagen)
Description: The Novagen® BacMagic™-1 transfection kit provides a positive selection for creating baculovirus recombinants in preparation for recombinant protein expression in insect cells. The kit improves on the traditional method for generating recombinant baculoviruses by eliminating the tedious, time-consuming steps of plaque purification.
UOM: 1 * 1 KIT
Image Unavailable-PRSI40-566

Human recombinant NT4 (from E. coli)

Catalog Number: (PRSI40-566)
Supplier: ProSci Inc.
Description: NT-4 is a neurotrophic factor structurally related to β-NGF, BDNF, and NT-3. These proteins belong to the cysteine-knot family of growth factors that assume stable dimeric structures. NT-4 is expressed in the prostate, thymus, placenta and skeletal muscle. NT-4 can signal through the LNGFR and trkB receptors and promotes the survival of peripheral sensory sympathetic neurons. Recombinant human NT-4 is a noncovalently linked homodimer, of two 14.0 kDa polypeptide monomers (260 total amino acid residues). Manufactured using all non-animal reagents.
UOM: 1 * 10 µG
Product Image-RPN1236

Anti-GST Antibody (HRP (Horseradish Peroxidase))

Catalog Number: (RPN1236)
Supplier: Cytiva
Description: GST is a commonly used expression marker in cloning that is also used for purification and detection of fusion proteins. This highly specific antibody to GST is conjugated to horseradish peroxidase and optimised for use in Western blotting with ECL™ Detection Reagents. The conjugate recognises multiple epitopes of GST and therefore is not reliant on functional GST for fusion protein detection. It has also been cross-absorbed to <i>E. coli </i>proteins so that GST-fusion proteins can be easily and specifically identified against an <i>E. coli</i> background.
UOM: 1 * 75 µl
Image Unavailable-PRSI40-557

Human recombinant IFNG (from E. coli)

Catalog Number: (PRSI40-557)
Supplier: ProSci Inc.
Description: IFN-γ is an acid-labile interferon produced by CD4 and CD8 T lymphocytes as well as activated NK cells. IFN-γ receptors are present in most immune cells, which respond to IFN-γ signaling by increasing the surface expression of class I MHC proteins. This promotes the presentation of antigen to T-helper (CD4+) cells. IFN-γ signaling in antigen-presenting cells and antigen-recognizing B and T lymphocytes regulate the antigen-specific phases of the immune response. Additionally, IFN-γ stimulates a number of lymphoid cell functions including the anti-microbial and anti-tumor responses of macrophages, NK cells, and neutrophils. Human IFN-γ species-specific and is biologically active only in human and primate cells. Recombinant human IFN-γ is a 16.7 kDa protein containing 143 amino acid residues.Manufactured using all non-animal reagents.
UOM: 1 * 100 µG
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L(+)-Cysteine, white powder cell culture reagent

Supplier: MP Biomedicals
Description: Storage: Store at Room Temperature (15-30 °C). Store Desiccated. Store under nitrogen.
L-Cysteine is a proteinogenic amino acid incorporated into proteins as directed by the genetic code. The thiol-side chain participates in a variety of oxidation/reduction reactions within the cell. The side chain participates in the formation of β bonds that modulate the secondary and ternary structure of proteins. Cysteine is essential and limiting for the formation of glutathione, an important antioxidant, within the cell.
Cysteine has been used in pulse-chase experiments to study protein expression from an inducible promoter. L-Cysteine is used to supplement cell culture media.
NMDA glutamatergic receptor agonist.

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Protein extraction reagents, BugBuster® and BugBuster® plus Benzonase®, Millipore®

Supplier: Merck Millipore (Novagen)
Description: BugBuster® Protein Extraction reagent is formulated for the gentle disruption of the cell wall of <i>E. coli</i>, liberating soluble protein. It provides a simple, rapid, low-cost alternative to mechanical methods such as French Press or sonication for releasing expressed target protein in preparation for purification or other applications. The formulation utilises a detergent mix that is capable of cell wall perforation without denaturing soluble protein.

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IPTG (isopropyl-β-D-thiogalactopyranoside) ≥99%, white powder

Supplier: MP Biomedicals
Description: IPTG (isopropyl-β-thiogalactoside) is an analogue of allolactose that binds specifically to the repressor protein of the lac operon and induces expression of β-galactosidase in Escherichia coli.
IPTG is a commonly used reagent in cloning procedures that require induction of b-galactosidase activity and is used in conjunction with X-Gal in blue-white color selection of recombinant bacterial colonies.
The final concentration typically used in indicator plates is 0.2 mM.

Image Unavailable-PRSI40-574

Human recombinant TGF beta 3 (from E. coli)

Catalog Number: (PRSI40-574)
Supplier: ProSci Inc.
Description: The three mammalian isoforms of TGF-β, TGF-β1, β2, β3, signal through the same receptor and elicit similar biological responses. They are multifunctional cytokines that regulate cell proliferation, growth, differentiation and motility as well as synthesis and deposition of the extracellular matrix. They are involved in various physiological processes including embryogenesis, tissue remodeling and would healing. They are secreted predominantly as latent complexes which are stored at the cell surface and in the extracellular matrix. The release of biologically active TGF-β isoform from a latent complex involves proteolytic processing of the complex and /or induction of conformational changes by proteins such as thrombospondin-1. The physiological role of TGF-β3 is still unknown but its expression pattern suggests a role in the regulation of certain development processes. Recombinant TGF-β3 is a 25.0 kDa protein composed of two identical 112 amino acid polypeptide chains linked by a single disulfide bond.Manufactured using all non-animal reagents.
UOM: 1 * 10 µG
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Deoxyoligo B2, BIOARRAY™

Catalog Number: (ENZOENZ42662150)
Supplier: ENZO LIFE SCIENCES
Description: BIOARRAY™ Deoxyoligo B2 is a synthetic biotin-labelled sequence that can be used in eukaryotic expression assays and in aiding software programs by aligning the grid with the array image.
UOM: 1 * 1 Vial
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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on +353 1 88 22222
This product is marked as restricted and can only be purchased by approved Shipping Accounts. If you need further assistance, email VWR Regulatory Department at eurega_services@eu.vwr.com
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