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Product Image-OZBILL73000

siRNA transfection reagent, Lullaby™

Supplier: OZ BIOSCIENCES
Description: Lullaby™ is an ideal siRNA transfection reagent for gene silencing. Based on the TEE-technology, it has been successfully tested on numerous cell lines, reaching up to 90% gene silencing with high reproducibility and very low toxicity. RNA interference is a powerful technique to shut down genes expression in cells and organisms. This silencing effect constitutes a very helpful tool to study gene function and a promising approach for new therapeutic treatments.

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Reagent kits for protein and fibre analysis

Catalog Number: (1.12979.0001)
Supplier: Merck
Description: Simple reagent kits, used in food analysis, that use colour chemistry to determine levels of protein and fibre. Please note these kits require the use of a photometer or spectrophotometer.
UOM: 1 * 100 Tests

MSDS Certificates


Product Image-MILF2500-S

Chemicon® ReBlot Plus Kits

Supplier: Merck
Description: Western blotting is a commonly used technique for studying protein function and localisation. Typically, protein samples are electrophoresed on SDS-page and transferred to a membrane such as nitrocellulose or nylon, where they are probed with specific antibodies. Unlike nucleic acid based technologies, which allow reuse of southern and northern blots, it has been difficult to reuse western blots.

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TAQuest™ FAST qPCR Master Mix for TaqMan Probes *High ROX*

Supplier: AAT BIOQUEST
Description: TAQuest™ FAST qPCR Master mix for TaqMan probes is a ready-to-use 2X solution optimised for qPCR and two-step RT-qPCR well suited to use for TaqMan gene expression assays.

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T7 RNA polymerase

Catalog Number: (EP0111)
Supplier: Thermo Fisher Scientific
Description: T7 RNA polymerase is ideal for synthesis of unlabeled and labeled RNA. It catalyses the 5'→3' synthesis of RNA on either single-stranded DNA or double-stranded DNA downstream from it promoter. It is also used in the study of RNA secondary structure and RNA-protein interactions, RNA splicing.
UOM: 1 * 1 KIT
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J.T.Baker® Endonuclease

Supplier: Avantor
Description: J.T.Baker® Endonuclease Biotech Reagent meets the strictest cGMP standards and is designed for the degradation of both single stranded and double stranded DNA and RNA. J.T.Baker® Endonuclease Biotech Reagent is used to ensure host cell DNA impurities are removed; driving process efficiency by lowering viscosity and preventing aggregation. J.T.Baker® Endonuclease Biotech Reagent is an enzyme based upon the native endonuclease of Serratia marcescens, enabling rapid clearance of residual DNA and RNA during the production and purification of both recombinant proteins and viral vectors. Non-animal origin. Absence of proteolytic activity. The purity of materials in non-negotiable. J.T.Baker® Endonuclease Biotech Reagent acts to degrade and eliminate extraneous genetic material, ensuring the pristine quality of your final product.

MSDS Certificates

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Amine reactive biotin reagents, HOOK™

Supplier: G-Biosciences
Description: HOOK™ d-Biotin (Vitamin H), 500 mg

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Taq DNA polymerase, MP Biomedicals

Supplier: MP Biomedicals
Description: <i>Taq</i> DNA Polymerase is a specialised thermostable enzyme isolated from the thermophilic bacterium <i>Thermus aquaticus</i>. The recombinant form of this enzyme is expressed in <i>E. coli</i>. It is highly processive, recombinant polymerase which catalyses the addition of mononucleotide units to the 3’-OH end of a primer chain. It remains functional even after prolonged incubation at 95 °C. This enzyme possesses 5’-3’ exonuclease activiy.

Product Image-0340-1G

D-(+)-Biotin ≥99%, Reagent Grade

Supplier: VWR Chemicals
Description: A small biomolecule, 244 Daltons, that has high binding affinity to avidin and streptavidin often used to label antibodies and enzymes. Several molecules of biotin can react with a single protein molecule to increase the sensitivity at the site of detection.

MSDS Certificates

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NEDD8-AMC

Catalog Number: (ENZOBMLUW00500025)
Supplier: ENZO LIFE SCIENCES
Description: NEDD8-AMC is a fluorogenic substrate for deNEDDylating enzymes, including NEDP1 and UCH-L3. It is a particularly useful reagent for the study of deNEDDylating activity where detection sensitivity or continuous monitoring of activity is essential.
UOM: 1 * 25 µG
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One step RT-PCR master mix kit

Catalog Number: (71978-3)
Supplier: Merck Millipore (Novagen)
Description: The One Step RT-PCR Master Mix Kit allows rapid, sensitive analysis of gene expression from tissues and cells. It can replace methods for detecting and quantifying gene expression such as Northern blots,<i> in situ</i> hybridisation, dot blots, S nuclease assays and conventional two step RT-PCR. The kit utilises recombinant <i>Thermus thermophilus</i> (rTth) DNA Polymerase, which acts as both a thermostable RNA dependent DNA polymerase and a DNA dependent DNA polymerase. The rTth DNA Polymerase is provided in a 2X master mix with an antibody for antibody-mediated hot start, optimised buffer, and ultrapure deoxynucleotides. Antibody-mediated Hot Start enhances specificity of both reverse transcription and PCR. The kit enables cDNA synthesis from input RNA followed by PCR amplification of the cDNA in a single reaction, with no additional hands-on requirement for buffer changes or adding reagents. Typically, detection of a specific transcript requires only 2 hours.
UOM: 1 * 1 KIT
Product Image-BB01

Botanical garden blot

Catalog Number: (BB01)
Supplier: G-Biosciences
Description: G-Biosciences' Botanical Garden Blot is prepared using total protein extracted from a variety of 5 to 10 day old, whole plant tissues.
UOM: 1 * 1 items
Product Image-MILF2504

Chemicon® ReBlot Plus Strong Antibody Stripping Solution, 10X

Catalog Number: (MILF2504)
Supplier: Merck
Description: Western blotting is a commonly used technique for studying protein function and localisation. Typically, protein samples are electro-phoresed on SDS-page and transferred to a membrane such as nitrocellulose or nylon, where they are probed with specific antibodies. Unlike nucleic acid based technologies, which allow reuse of southern and northern blots, it has been difficult to reuse western blots.
UOM: 1 * 50 mL
Product Image-ROCKPA00-03

Avidin, biotin, protein A/G and streptavidin reagents

Supplier: Rockland Immunochemicals
Description: Avidin is biotin-binding protein, tetramer and suitable for used as a antigen, control and standard in assays. Avidin fluorescein, rhodamine, alkaline phosphatase, Texas Red™ conjugated are suitable for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent Western blotting. Avidin conjugated with glucose oxidase and peroxidase are useful as a detection reagent for primary antibodies conjugated with biotin and also can be utilised in Western blotting and ELISA experiments with proper substrates.

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DNA transfection reagent, Magnetofectamine™ O2

Supplier: OZ BIOSCIENCES
Description: Magnetofectamine™ O2 is a powerful transfection kit designed for primary and hard-to-transfect cells. The alliance of MTX transfection reagent and CombiMag leads to increased transfection efficiency, minimised toxicity and enhanced gene expression. CombiMag, a magnetic formulation based on the Magnetofection™ technology, binds to MTX transfection reagent/DNA complexes and under the application of a magnetic field concentrates the genetic material onto cells and promotes cellular uptake. In this way, transfection efficiency is enhanced.

Product Image-TB23

Mouse single tissue blot, single species

Supplier: G-Biosciences
Description: This blot contains two protein lanes, a protein marker lane and a lane with one GenLysate™ sample. After the proteins are resolved, they are transferred to a PVDF membrane using Western Transfer Buffer. The blots are ready to be blocked and probed with the antibodies of choice.

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on +353 1 88 22222.
Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on +353 1 88 22222
This product is marked as restricted and can only be purchased by approved Shipping Accounts. If you need further assistance, email VWR Regulatory Department at eurega_services@eu.vwr.com
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This product has been blocked by your organisation. Please contact your purchasing department for more information.
The original product is no longer available. The replacement shown is available.
Product(s) marked with this symbol are discontinued - sold till end of stock. Alternatives may be available by searching with the VWR Catalog Number listed above. If you need further assistance, please call VWR Customer Service on +353 1 8822222.
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