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DNA extraction, MPure™ Blood DNA extraction kits, MP Biomedicals

Supplier: MP Biomedicals
Description: MPure™ Blood DNA Extraction Kit is used with the MPure-12™ aNAP System for extraction of DNA from 100 to 400 µl mammalian whole blood, peripheral blood mononuclear cells or buffy coat. Nucleic acids extracted from MPure Blood DNA Extraction Kit 200 can be used in a number of downstream application including: PCR, qPCR, Sequencing (NGS), Microarray, RFLP, Southern Blot Analysis.

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Plasmid Midiprep kit, GeneJET™

Supplier: Thermo Fisher Scientific
Description: GeneJET™ Plasmid Midiprep Kit is designed for large scale isolation of high quality plasmid DNA from recombinant <i>E. coli</i> cultures. The kit utilises silica-based membrane technology in the form of convenient spin column. Each prep recovers up to 200 µg of high copy number plasmid DNA. The purified plasmid DNA can be used in a wide variety of molecular biology procedures, such as restriction endonuclease digestion, PCR, cloning, transformation, automated sequencing,<i> in vitro</i> transcription, and transfection of robust cell lines.

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SPINeasy® Host Depletion Microbial DNA Kit, MP Biomedicals

Catalog Number: (116545050.)
Supplier: MP Biomedicals
Description: Host DNA contamination impedes the molecular analyses of microbiomes in host samples, such as bodily fluids and swabs. The SPINeasy Host Depletion Microbial DNA kit provides an easy-to-use workflow to isolate microbial DNA from samples containing high amounts of host DNA.
UOM: 1 * 1 KIT
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MagBeads FastDNA™ Kit for Feces

Catalog Number: (116570400.)
Supplier: MP Biomedicals
Description: <p>The MagBeads FastDNA Kit for Feces allows thorough sample lysis and quick isolation of DNA from fecal samples. It is using magnetic beads with high DNA binding capacity for extraction of high yields of pure DNA. This is done manually using a magnetic rack or adapted to automated instruments for high throughput processing.</p>
UOM: 1 * 50 items
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Plant Genomic DNA purification Mini kit, GeneJET™

Supplier: Thermo Fisher Scientific
Description: GeneJET™ Plant Genomic DNA Purification Mini Kit is designed for rapid and efficient purification of high quality genomic DNA from a wide variety of plant species and tissue types. It utilises silica-based membrane technology in the form of a convenient spin column, eliminating the need for expensive resins, toxic phenol-chloroform extractions, or time-consuming alcohol precipitation. The standard procedure takes less than 30 minutes following cell lysis and yields purified DNA greater than 30 kb in size. DNA yields vary between different species and tissues depending on genome size, ploidy, cell number, and the age of the tissue sample. The typical yield from a standard tissue source (for example, young wheat leaves) is 30 to 32 μg from 100 mg of tissue. Isolated DNA can be used directly in PCR, qPCR, Southern blotting, and enzymatic reactions.

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Fast RNA™ Pro Soil-Indirect Kit, MP Biomedicals

Catalog Number: (ICNA116075050)
Supplier: MP Biomedicals
Description: FastRNA™ Pro Soil-Indirect Kit is used to isolate total RNA from organic material found in soil samples. This kit allows for the sample to be slurried with water or buffer in order to further separate biomass from the soil or environmental matrix, and the resulting supernatant is then filtered, lysed, and purified through the rest of the protocol. Total RNA isolated is suitable for RT-PCR analysis and other downstream applications.
UOM: 1 * 50 Tests
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illustra™ plasmidPrep Mini Spin kit

Supplier: Cytiva
Description: The illustra™ plasmidPrep Mini Spin Kit uses a simple plasmid DNA purification protocol involving a modified alkaline lysis procedure and a novel silica-based membrane to achieve highly efficient plasmid DNA purification. Produces high quality plasmid DNA with excellent reproducibility for use in cloning, restriction enzyme digestion, PCR amplification, and DNA sequencing.

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Gel DNA kit, SpinPrep™

Catalog Number: (70852-3)
Supplier: Merck Millipore (Novagen)
Description: The SpinPrep™ Gel DNA Kit enables efficient extraction of DNA fragments of 150 bp to >12000 bp in size from agarose gels. The procedure uses GelMelt™ solution to dissolve the gel slice, followed by adsorption of the DNA to a silica membrane in a spin column format. After a wash step, the purified DNA is eluted in low salt buffer. Each spin column can bind up to 20 µg DNA. Recoveries are consistently in the range of 50 to 90% under standard conditions.
UOM: 1 * 100 Tests
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Dye terminator removal, illustra™ AutoSeq™ G-50 columns

Supplier: Cytiva
Description: illustra™ AutoSeq™ G-50 columns are specifically designed to remove fluorescent dye-terminators from sequencing reactions prior to analysis on automated sequencers. Effective purification is essential for high quality sequencing results since residual dye-terminators can obscure data. illustra™ AutoSeq™ G-50 consists of MicroSpin™ columns containing Sephadex™ G-50, pre-equilibrated in double-distilled water. This is important because even the small amount of salt in traditional buffers (such as TE buffer) can cause electrophoretic artifacts on salt-sensitive automated sequencers.

MSDS

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Pellet Paint® NF Co-Precipitant

Supplier: Merck Millipore (Novagen)
Description: Pellet Paint® NF Co-Precipitant is a non-fluorescent dye-labelled carrier compatible with fluorescent sequencing. It facilitates rapid removal of BigDye® Terminators during the alcohol precipitation of cycle sequencing reaction products. Cycle sequencing reactions can be precipitated rapidly with centrifugation times of 10 minutes. The easily visualised carrier provides a simple confirmation that precipitation has occurred. Sequencing reaction products are efficiently pelleted and dye-labelled terminators remain in the supernatant during alcohol precipitation using the standard Applied Biosystems precipitation protocols. Re-suspension of pelleted sequencing reaction products in deionised formamide can be confirmed by checking for dissolution of the carrier pellet.

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Genomic DNA isolation and purification kits, GET™ Plant DNA Template

Catalog Number: (786-355)
Supplier: G-Biosciences
Description: GET™ Plant DNA Template is a spin column format kit suitable for the preparation of DNA templates from plant samples. The isolated genomic DNA is suitable for PCR and other applications. GET™ Plant DNA Template purifies plant genomic DNA free from plant impurities known to inhibit downstream applications. The high yield, extracted DNA is normally >50 kb in size. The yield is ~30 µg DNA per preparation, depending on the plant source and quantity used.
UOM: 1 * 100 Tests
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Pellet Paint® Co-Precipitant

Supplier: Merck Millipore (Novagen)
Description: Pellet Paint® Co-Precipitant is a visible dye-labelled carrier formulated specifically for use in alcohol precipitation of nucleic acids. The 2-minute precipitation does not require low-temperature incubations or prolonged centrifugation. Both RNA and DNA are efficiently precipitated from even the most dilute solutions (2 ng/ml) and the pellet is easily located by its vivid pink colour. The pellet can be easily followed during washing steps and this prevents losses during handling and ensures thorough pellet re-suspension.

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SPINeasy® DNA Kit for Tissue (with Lysing Matrix)

Catalog Number: (116558050.)
Supplier: MP Biomedicals
Description: <p>SPINeasy® DNA Kit for Tissue (with Lysing Matrix) is a high-performance genomic DNA (gDNA) extraction kit which combines mechanical, chemical and enzymatic disruption of tissue samples in 30 minutes. The addition of proteinase K allows a more efficient and complete digestion of any tissue type. Homogenizing time is greatly reduced while substantially increasing the gDNA yield and purity. The kit is validated for tissue samples of fatty, tough, and fibrous samples, and suitable for downstream applications such as PCR and qPCR.</p>
UOM: 1 * 50 Tests

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illustra™ ProbeQuant™ G-50 micro columns

Catalog Number: (28-9034-08)
Supplier: Cytiva
Description: ProbeQuant™ G-50 micro columns use spin column chromatography to provide a fast and reliable method for purifying labelled probes from unincorporated labelled nucleotides. In addition, a portion of the eluted DNA can be counted in a scintillation counter and compared to an unpurified sample to give an approximation of percent incorporation. The columns are designed for use in a microcentrifuge, come prepacked with Sephadex™ G-50 DNA grade and are pre-equilibrated in STE, which means that they are ready to use. They can accommodate samples ranging in volume from 25 μl to 50 μl.
UOM: 1 * 50 items
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Bacteria genomicPrep mini spin kit, illustra™

Supplier: Cytiva
Description: The illustra™ bacteria genomicPrep Mini Spin Kit is designed for the rapid extraction and purification of high quality genomic DNA from gram-positive and gram-negative bacteria. The kit can be used to isolate genomic DNA from a wide range of bacterial strains such as DH5a, TOP10, JM109, <i>Bacillus subtilis</i>, etc. The illustra™ bacteria genomicPrep Mini Spin Kit produces highly pure and intact genomic DNA with A<sub>260</sub>/A<sub>280</sub> values of 1,75 to 1,85.

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SpinPrep™ PCR clean up kit

Catalog Number: (70976-3)
Supplier: Merck Millipore (Novagen)
Description: The SpinPrep™ PCR Clean Up Kit is designed for the rapid purification of DNA amplified by PCR. The 10-minute procedure involves addition of a binding buffer followed by adsorption of the DNA to a silica membrane in a spin column format. Following a wash step, the DNA is eluted in low salt buffer. This kit removes DNA polymerises, dNTPs, salts, and >99% of primers so that they do not interfere with downstream applications such as cloning, sequencing, or labelling. PCR products from 100 to >12000 bp can be cleaned up, with recoveries of amplified DNA in the range of 60 to 90% under standard conditions.
UOM: 1 * 100 Tests
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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on +353 1 88 22222
This product is marked as restricted and can only be purchased by approved Shipping Accounts. If you need further assistance, email VWR Regulatory Department at eurega_services@eu.vwr.com
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This product has been blocked by your organisation. Please contact your purchasing department for more information.
The original product is no longer available. The replacement shown is available.
Product(s) marked with this symbol are discontinued - sold till end of stock. Alternatives may be available by searching with the VWR Catalog Number listed above. If you need further assistance, please call VWR Customer Service on +353 1 8822222.
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