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You Searched For: Next+Generation+Sequencing+Reagents


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Product Image-AGLSG9700B

NGS FFPE QC kit

Supplier: AGILENT
Description: The Agilent NGS FFPE QC kit is a qPCR-based assay that enables functional DNA quality assessment of input DNA prior to preparation of next generation sequencing (NGS) libraries.

Product Image-665-0562

Multiparameter photometer, Water-ID® The PrimeLab 2.0

Supplier: WATER ID PRIMELAB
Description: The next generation of multitest Photometer, with pH - EC - TDS - ORP - Temperature measurement functionality.

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Product Image-MAG-PCR-CL-50

PCR clean-up kit, Axygen® AxyPrep™ Mag

Supplier: Corning
Description: The efficient removal of unincorporated dNTPs, salts and enzymes after any PCR is detrimental to the success or failure of downstream applications, such as genotyping, Sanger, and Next Generation Sequencing. The AxyPrep™ Mag PCR Clean Up Kit utilises a paramagnetic bead technology for rapid high throughput purification of PCR products. Additionally, the AxyPrep™ Mag PCR Clean Up Kit employs an optimised buffer to selectively bind PCR amplicons 60 bp and larger to paramagnetic beads, providing end users flexibility and high recovery options. The protocol comprises binding, washing and eluting steps, where primers, nucleotides, salts and enzymes in the reaction mixture are efficiently removed leaving a purified PCR product that is essentially free of contaminants. No centrifugation or filtration steps are required, allowing the process to be adapted to multiple commercially available automation platforms.

Product Image-QUNT95171-010

Genomic DNA extraction from dried blood spots, Extracta™ DBS

Supplier: Quantabio
Description: Extracta™ DBS is a ready to use DNA extraction reagent for rapid and efficient recovery of PCR-ready DNA from dried blood spots (DBS) on Guthrie cards or Whatman® 903 filter/TFN or 226 from Ahlstrom Munksjö paper. This patented single-solution process produces DNA eluates that are substantially free of PCR inhibitors and compatible with a variety of end-point PCR, real-time PCR and Next Generation Sequencing (NGS) or Sanger Sequencing reagents. Application of Extracta DBS with PerfeCTa® qPCR ToughMix® or PerfeCTa MultiPlex qPCR ToughMix® enables accurate and reproducible quantification of DNA sequences in blood using TaqMan® hydrolysis probe real-time qPCR.

MSDS

Product Image-QUNT95161-025

qScript™ XLT cDNA SuperMix

Supplier: Quantabio
Description: qScript™ XLT cDNA SuperMix is a next generation tool for first-strand cDNA synthesis, providing a highly sensitive and easy to use solution for two-step RT-PCR and RT-qPCR. qScript XLT is an engineered M-MLV reverse transcriptase with reduced RNase H activity and improved activity and stability at higher temperatures. Combined with a precise mixture of reaction components, this SuperMix enables superior results over a wide dynamic range of input RNA, with up to 8-fold higher sensitivity than cDNA synthesis kits utilising an RNase H(+) reverse transcriptase (RT).

MSDS

Product Image-M6399-01

Mag-Bind® HDQ blood and tissue DNA 96 Kit

Supplier: OMEGA BIO-TEK
Description: The Mag-Bind® Blood and Tissue DNA HDQ 96 Kit is designed for rapid and reliable isolation of high quality genomic DNA from 100 to 200 μl blood samples, buccal swabs, buffy coat and cultured cells. All heating steps that limit robotic applications have been removed to allow for faster processing. Mag-Bind® particles HDQ provide quick magnetic response time reducing overall processing time. Utilising paramagnetic particles provides high quality DNA that is suitable for direct use in most downstream applications, such as qPCR, PCR and Next Generation Sequencing. The Mag-Bind® HDQ Blood DNA and Tissue 96 Kit is compatible with many robotic liquid handlers and magnetic processors, including Thermo Scientific KingFisher® Flex.

MSDS Certificates

Image Unavailable-29208-50MG

(N-Succinimidyloxycarbonylmethyl)tris(2,4,6-trimethoxyphenyl)phosphonium bromide acylation reagent, for protein sequencing by MALDI-MS, Supelco®

Catalog Number: (29208-50MG)
Supplier: Merck
Description: N-Succinimidyloxycarbonylmethyl tris(2,4,6-trimethoxyphenyl)phosphonium bromide (TMPP-Ac-OSu) is an acylating reagent, and is generally used for the selective derivatization of N-terminal a-amino group of peptide.
UOM: 1 * 50 mg
Product Image-AATB17092

7-Deaza-7-Propargylamino-3'-azidomethyl-dGTP

Catalog Number: (AATB17092)
Supplier: AAT BIOQUEST
Description: 7-Deaza-7-Propargylamino-3'-azidomethyl-dGTP is a key building block for preparing fluorescent conjugates that are used in the next generation sequencing (NGS).
UOM: 1 * 50 Nmo
Product Image-AATB17090

7-Deaza-7-Propargylamino-3'-azidomethyl-dATP

Catalog Number: (AATB17090)
Supplier: AAT BIOQUEST
Description: 7-Deaza-7-Propargylamino-3'-azidomethyl-dATP is a key building block for preparing fluorescent conjugates that are used in the next generation sequencing (NGS).
UOM: 1 * 50 Nmo
Product Image-AATB17091

5-Propargylamino-3'-azidomethyl-dCTP

Catalog Number: (AATB17091)
Supplier: AAT BIOQUEST
Description: 5-Aminopropargyl 3’-azidomethyl dCTP is a key building block for preparing fluorescent conjugates that are used in the next generation sequencing (NGS).
UOM: 1 * 50 Nmo
Product Image-AATB17093

5-Propargylamino-3'-azidomethyl-dUTP

Catalog Number: (AATB17093)
Supplier: AAT BIOQUEST
Description: 5-Propargylamino-3'-azidomethyl-dUTP is a key building block for preparing fluorescent conjugates that are used in the next generation sequencing (NGS).
UOM: 1 * 50 Nmo
Image Unavailable-ENZOENZ42502

Cyanine 5-dUTP

Catalog Number: (ENZOENZ42502)
Supplier: ENZO LIFE SCIENCES
Description: Cyanine 5-dUTP can replace TTP in reactions in which it serves as a substrate for E. coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase. Fluorescently labeled probes can be prepared with this fluorescent nucleotide by a variety of methods including nick translation, cDNA labeling and 3’-end labeling. Probes generated by these methods are suitable for use for the identification of specific sequences by in situ hybridization procedures on fixed cells and tissues by direct fluorescence detection. Cyanine 5-dUTP can also be used for multicolor fluorescence labeling.
UOM: 1 * 25 Nmo

New Product


Product Image-ENZOENZ42501

Cyanine 3-dUTP

Catalog Number: (ENZOENZ42501)
Supplier: ENZO LIFE SCIENCES
Description: Cyanine 3-dUTP can replace TTP in reactions in which it serves as a substrate for E. coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase. Fluorescently labeled probes can be prepared with this fluorescent nucleotide by a variety of methods including nick translation, cDNA labeling and 3’-end labeling. Probes generated by these methods are suitable for use for the identification of specific sequences by in situ hybridization procedures on fixed cells and tissues by direct fluorescence detection. Cyanine 3-dUTP can also be used for multicolor fluorescence labeling.
UOM: 1 * 25 Nmo

New Product


Product Image-BSENM-1311-100

Anti-His Tag Mouse Monoclonal Antibody [clone: BHISH8]

Catalog Number: (BSENM-1311-100)
Supplier: Biosensis
Description: The 6X His tag is a short peptide sequence of 6 histidine residues. Epitopes such as the 6X His tag are often included with the target DNA at the time of cloning to produce fusion proteins containing the tag sequence. This allows anti-epitope tag antibodies such as this one to serve as a universal detection reagent for any recombinant protein containing this tag. Anti-epitope antibodies are a useful alternative to generating antibodies to identify a specific recombinant protein. The 6X His motif is often used as a tag on recombinant proteins to facilitate purification with immobilized metal-affinity chromatography.
UOM: 1 * 100 µG
Product Image-BSENR-1312-100

Anti-His Tag Rabbit Polyclonal Antibody

Catalog Number: (BSENR-1312-100)
Supplier: Biosensis
Description: The 6X His tag is a short peptide sequence of 6 histidine residues. Epitopes such as the 6X His tag are often included with the target DNA at the time of cloning to produce fusion proteins containing the tag sequence. This allows anti-epitope tag antibodies such as this one to serve as a universal detection reagent for any recombinant protein containing this tag. Anti-epitope antibodies are a useful alternative to generating antibodies to identify a specific recombinant protein. The 6X His motif is often used as a tag on recombinant proteins to facilitate purification with immobilized metal-affinity chromatography.
UOM: 1 * 100 µG
Image Unavailable-BOSSBS-5364R

Anti-HSF1 Rabbit Polyclonal Antibody

Catalog Number: (BOSSBS-5364R)
Supplier: Bioss
Description: Two HSFs have been identified in human cells, HSF 1 and HSF 2, which bind to the same HSEs and have 38% sequence identity. These factors are activated by distinct stimuli, HSF 1 is responsive to classical stress signals such as heat, heavy metals and oxidative reagents, whereas HSF 2 is activated during hemin-mediated differentiation of human erythroleukemia cells.HSF 1 exists constitutively in the cytoplasm and the nucleus of unstressed cells as a monomer which lacks DNA binding activity. Through an unknown signal generated during stress, HSF 1 becomes activated to a nuclear localized, trimeric state which binds to DNA. The phosphorylation of HSF 1 is necessary for maximal transcription of heat shock genes.
UOM: 1 * 100 µl
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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on +353 1 88 22222
This product is marked as restricted and can only be purchased by approved Shipping Accounts. If you need further assistance, email VWR Regulatory Department at eurega_services@eu.vwr.com
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This product has been blocked by your organisation. Please contact your purchasing department for more information.
The original product is no longer available. The replacement shown is available.
Product(s) marked with this symbol are discontinued - sold till end of stock. Alternatives may be available by searching with the VWR Catalog Number listed above. If you need further assistance, please call VWR Customer Service on +353 1 8822222.
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