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Product Image-EDVO223/AP08

Transformation with green fluorescent protein

Catalog Number: (EDVO223/AP08)
Supplier: EDVOTEK EUROPE LTD
Description: In this experiment, transformed cells take up a plasmid containing the Green Fluorescent Protein (GFP) gene. The GFP gene was isolated from the jellyfish <i>Aequorea victoria</i>. Transformed colonies expressing the GFP protein are visibly green in normal light but will fluoresce brightly when exposed to longwave UV light.
UOM: 1 * 1 items
Image Unavailable-EDVO616

ReadyPour™ Luria Broth (LB) agar with ampicillin

Catalog Number: (EDVO616)
Supplier: EDVOTEK EUROPE LTD
Description: Educational Materials, Microbiology, Application: Microbiology, ReadyPour™ LB agar with ampicillin
UOM: 1 * 170 mL

Collection devices for clinical microbiology

Complete portfolio of high-quality swabs to collect bacteria for clinical bacteriology.

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Gel-media swabs

Improve collection quality
Image Unavailable-EDVO615

ReadyPour™ Luria Broth (LB) agar base

Catalog Number: (EDVO615)
Supplier: EDVOTEK EUROPE LTD
Description: Educational Materials, Microbiology, Application: Microbiology
UOM: 1 * 1 mL
Product Image-EDVO221

Transformation of E. coli with pGAL™

Catalog Number: (EDVO221)
Supplier: EDVOTEK EUROPE LTD
Description: In this experiment, students see a blue colour change in transformed cells due to the switching on of a gene. The pGAL plasmid gives a blue colour dye due to the production of the β-galactosidase protein by the <i>lacZ</i> gene. IPTG is not required in this experiment since pGAL contains the complete <i>lacZ</i> gene.
UOM: 1 * 1 items
Product Image-BE-301

Experimentation kits, bacterial conjugation

Catalog Number: (BE-301)
Supplier: G-Biosciences
Description: Bacterial conjugation is a naturally occurring process that allows the transfer of DNA from one bacterium to another, which allows the transfer of genetic traits, particularly drug resistance. The kit contains two bacteria with different drug resistance genes and all the tools for students to study bacterial conjugation. This kit teaches the difference between bacterial genomic DNA and the transferable plasmid DNA and the mechanisms of bacterial conjugation. Students will also learn important basic microbiological techniques, including bacterial growth in liquid broth and on solid agar plates, antibiotic selection of bacteria and important aseptic techniques.
UOM: 1 * 1 KIT
Product Image-BE-203

Experimentation kits, antibiotic sensitivity and bacteria screening

Catalog Number: (BE-203)
Supplier: G-Biosciences
Description: Antibiotics play a crucial role in the manipulation, screening and killing of bacteria in a range of biotechnology processes. This kit specifically teaches the basic principles of antibiotics, bacterial resistance and susceptibility. Students learn and understand the use of antibiotic resistance in screening for infectious diseases. Utilising a bacterial strain, students learn the effects of different antibiotics and visualise bacterial sensitivity and resistance to the supplied antibiotics. This method involves the use of filter paper discs impregnated with a specified concentration of antibiotics on the surface of an agar plate containing microbial cells. This kit will enable students to analyse the inhibitory effects of different antibiotics on selected bacterial cells and then determine which antibiotic is the most suitable to treat a bacterial infection.
UOM: 1 * 1 KIT
Product Image-BE-201

Experimentation kits, bacterial culture and growth study

Catalog Number: (BE-201)
Supplier: G-Biosciences
Description: This kit teaches aseptic handling techniques and cultivation of bacteria in liquid culture media and on solid phase agar plates. This kit is designed to educate students about the various stages of the bacterial growth cycle, i.e. lag, log or exponential, stationary and decline or death phases. The kit also teaches the importance of growing bacteria on solid phase agar plates to isolate single colonies of bacteria. This lab activity involves preparation of culture medium and solid agar plates.
UOM: 1 * 1 KIT
Product Image-BE-202

Experimentation kits, bacterial gram staining

Catalog Number: (BE-202)
Supplier: G-Biosciences
Description: The Gram staining method was first described in 1844 by the Danish bacteriologist Hans Christian Gram, after whom the test was named. The Gram staining test for bacteria is one of the most important tests in microbiology and is often one of the first tests performed in the identification of bacteria. The Gram staining method utilises the properties of bacterial cell walls and the stain crystal violet. Gram-positive microorganisms have a higher peptidoglycan and lower lipid content than Gram-negative bacteria in their cell walls and this makes them capable of retaining the Gram stain. This kit is supplied with two strains of bacteria and all the necessary components to carry out the Gram staining.
UOM: 1 * 1 KIT

Certificates


Product Image-EDVO255

Purification and size determination of green and blue fluorescent proteins

Catalog Number: (EDVO255)
Supplier: EDVOTEK EUROPE LTD
Description: When bacteria are used to make medicinally useful proteins by transformation, the protein of interest must be separated from all of the other cellular proteins. In this experiment, the unique fluorescent properties of GFP and BFP are used as an assay during their purification from an <i>E. coli</i> extract. The column fractions containing GFP or BFP are identified by fluorescence and then purified. As an optional activity, purified protein fractions can be separated by SDS polyacrylamide gel electrophoresis (SDS-PAGE) to estimate the purity and size of the GFP and BFP proteins.
UOM: 1 * 1 KIT
Product Image-EDVO222

Transformation with blue and green fluorescent proteins

Catalog Number: (EDVO222)
Supplier: EDVOTEK EUROPE LTD
Description: The Green Fluorescent Protein (GFP) from the jellyfish <i>Aequorea victoria</i> is used extensively in all areas of science. Many organisms have been transformed with the GFP gene, the gene responsible for bioluminescence in jellyfish. It has proven to be so useful that scientists have mutated it to produce Blue Fluorescent Protein (BFP). In this simple experiment, students transform bacteria either by GFP, BFP or both.
UOM: 1 * 1 items

MSDS


Product Image-RDBT002015

VWR® Electrode Holders

Catalog Number: (RDBT002015)
Supplier: VWR Chemicals
Description: Plain borosilicate tubes for insertion of a wire electrode so that the electrode is insulated from the clamp or holder and protected from mechanical damage. The tube also helps the flexible wire electrode be positioned correctly in a rigid support.
UOM: 1 * 100 items
Product Image-BE-502

Experimentation kits, dot blot analysis

Catalog Number: (BE-502)
Supplier: G-Biosciences
Description: Dot blotting is a simple technique to identify a known protein in a biological sample. The ease and simplicity of the technique makes dot blotting an ideal diagnostic tool.
UOM: 1 * 1 KIT
Product Image-201-0216

Capillary tube on foot

Catalog Number: (201-0216)
Supplier: GLASWARENFABRIK KARL HECHT
Description: Five communicating glass tubes with different inner diameters. Used to show capillary forces and how they are affected by the inner diameter of the pipes.
UOM: 1 * 1 items
Product Image-BE-404

Experimentation kits, hydrophobic and hydrophilic proteins

Catalog Number: (BE-404)
Supplier: G-Biosciences
Description: This lab activity is designed to demonstrate the different classes of protein molecules and their classification based on solubility. Students learn fractionation of soluble, insoluble membrane proteins, and cytoskeleton proteins from a tissue sample. The insoluble protein fraction is further fractionated into hydrophilic and hydrophobic membrane proteins. Cell membrane structure and the role of hydrophobic membrane proteins are considered. This lab activity also provides an opportunity to understand characteristics of various classes of detergents and the role of detergents in solubilisation of hydrophobic membrane proteins.
UOM: 1 * 1 KIT
Product Image-765-0969

Communicating tubes

Catalog Number: (765-0969)
Supplier: GLASWARENFABRIK KARL HECHT
Description: Five glass tubes of different shape and inner dimensions. Down to each other. When filling the liquid in the pipes, it is clear that the height of the stowage in the tubes. pipes are independent of the design of the pipes. Comes with foot.
UOM: 1 * 1 items
Product Image-BE-401

Experimentation kits, physical properties of proteins

Catalog Number: (BE-401)
Supplier: G-Biosciences
Description: The physical properties of proteins kit is a lab activity that enables students to investigate the physical properties of several different proteins. Students will learn about protein solubility and how it is affected by various parameters; including temperature, pH, salt and dielectric constant. They will understand about protein precipitation due to pH, high salt and in the presence of organic solvents and about protein denaturation as a result of high temperature. In addition, the kit will demonstrate how non-protein agents, such as detergents drastically alter the physical properties of protein molecules and as a result, understand the importance of detergents in protein solubilisation. This lab activity involves analysis of three different types of pure proteins and then students alter some of those properties with a detergent and re-examine physical properties of those proteins. Students are challenged to consider how physical properties of protein molecules can be exploited for purification and characterisation of proteins and apply their findings on a test sample of complex tissue extract.
UOM: 1 * 1 KIT
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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on +353 1 88 22222.
Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on +353 1 88 22222
This product is marked as restricted and can only be purchased by approved Shipping Accounts. If you need further assistance, email VWR Regulatory Department at eurega_services@eu.vwr.com
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Product(s) marked with this symbol are discontinued - sold till end of stock. Alternatives may be available by searching with the VWR Catalog Number listed above. If you need further assistance, please call VWR Customer Service on +353 1 8822222.
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