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Image Unavailable-PRSI33-517

Anti-pan Cytokeratin Mouse Monoclonal Antibody [clone: KRTL/1077 + KRTH/1076]

Catalog Number: (PRSI33-517)
Supplier: ProSci Inc.
Description: Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This antibody cocktail recognises acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, which include Keratins 1, 3, 4, 5, 6, 8, 10, 14, 15, 16, and 19. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. KRTL/KRTH is a broad spectrum anti pan-cytokeratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. It has been used to characterize the source of various neoplasms and to study the distribution of cytokeratin containing cells in epithelia during normal development and during the development of epithelial neoplasms. This antibody stains cytokeratins present in normal and abnormal human tissues and has shown high sensitivity in the recognition of epithelial cells and carcinomas.
UOM: 1 * 100 µG

New Product


Image Unavailable-18-1129-58

Power supply adapters

Supplier: Cytiva
Description: Choice of adapters for connecting 4 or 2 mm leads to 2 or 4 mm output jacks, respectively.

Product Image-1.14296.0001

HPTLC plates silica gel 60, RP-modified layers, glass backed

Supplier: Merck
Description: Our NH₂, CN- and DIOL-modified silica sorbents are less polar then conventional silica phases, making them ideal for separating hydrophilic or charged substances. Our moderately polar cyano- and diol-modified silica plates can be used for both normal phase and reversed phase systems. An alternative to PEI cellulose, amino-modified NH₂ plates provide weak basic ion exchange characteristics with special selectivity for charged compounds.

MSDS

Product Image-EDVO316

Experimentation kits, in search of the cholesterol gene

Catalog Number: (EDVO316)
Supplier: EDVOTEK
Description: Coronary heart disease and stroke are major causes of death in the Western world. Elevated blood cholesterol levels are a serious risk factor in both conditions. The genetic disease familial hypersholesterolemia (FH) causes an increase in blood levels of the bad form of cholesterol, low density lipoprotein (LDL). In untreated patients with the mutant FH gene, the condition can cause premature death. This experiment introduces the colorimetric enzymatic reaction which is the basis of the clinical cholesterol test. In addition, using agarose gel electrophoresis, students can analyse a simulated genetic screening for a disease.
UOM: 1 * 1 KIT
Image Unavailable-BOSSBS-7166R-FITC

Anti-MUC5AC Rabbit Polyclonal Antibody (FITC (Fluorescein Isothiocyanate))

Catalog Number: (BOSSBS-7166R-FITC)
Supplier: Bioss
Description: Gel-forming glycoprotein of gastric and respiratoy tract epithelia that protects the mucosa from infection and chemical damage by binding to inhaled microrganisms and particles that are subsequently removed by the mucocilary system.
UOM: 1 * 100 µl
Image Unavailable-BOSSBS-7166R-A555

Anti-MUC5AC Rabbit Polyclonal Antibody (Alexa Fluor® 555)

Catalog Number: (BOSSBS-7166R-A555)
Supplier: Bioss
Description: Gel-forming glycoprotein of gastric and respiratoy tract epithelia that protects the mucosa from infection and chemical damage by binding to inhaled microrganisms and particles that are subsequently removed by the mucocilary system.
UOM: 1 * 100 µl
Image Unavailable-ICNA0219485794

Urea ≥98%, white powder for molecular biology

Supplier: MP Biomedicals
Description: Applications:
Urea is used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies and in the extraction of the mitochondrial complex. It is commonly used to solubilize and denature proteins for denaturing isoelectric focusing and two-dimensional electrophoresis and in acetic acid-urea PAGE gels. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form. Urea is used in cell or tissue culture media to increase the osmolality. Urea has also been used as fertilizer because of the easy availability of nitrogen; in animal feeds; it is reacted with aldehydes to make resins and plastics; condensed with malonic ester to form barbituric acid; used in the paper industry to soften cellulose.
Biochem/physiol Actions:
Urea has been shown to act as an aldosterone antagonist in the development of peanut agglutinin binding in cultured embryonic renal collecting duct epithelial cells. Urea is the principal end product of nitrogen metabolism in most mammals, formed by the enzymatic reactions of the Kreb's cycle.
Typical Working Concentration:
The use of 2 g/L urea in the culture of Kluyveromyces marxianus to produce a thermostable extracellular lipase has been described. Urea is typically used at a concentration of 8 M for protein denaturation or solubilization. A final concentration of 5 M urea is commonly used in molecular biology for sequencing gels. To prevent carbamylation, do not heat urea containing buffers above 37 °C

Product Image-PRSI36-346

Anti-VASP Rabbit Polyclonal Antibody

Catalog Number: (PRSI36-346)
Supplier: ProSci Inc.
Description: VASP (vasodilator-stimulated phosphoprotein) is a proline-rich protein substrate of cAMP- and cGMP-dependent protein kinases. VASP is an actin-associated protein involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation. It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping proteins. VASP stimulates actin filament elongation by promoting the transfer of profilin-bound actin monomers onto the barbed end of growing actin filaments and it plays a role in actin-based mobility of Listeria monocytogenes in host cells. It regulates actin dynamics in platelets and plays an important role in regulating platelet aggregation. VASP phosphorylation is used to monitor the effect of so-called antiplatelet drugs that reduce platelet reactivity and are used to prevent stent thrombosis, strokes and heart attacks in patients. Phosphorylation of VASP at Ser157 causes a mobility shift in SDS gel electrophoresis from 46 to 50kDa, which has been used as a convenient marker to monitor cyclic nucleotide-dependent protein kinase activity.
UOM: 1 * 25 µG
Product Image-460-0310

Digital block heater, with touch screen

Supplier: Thermo Fisher Scientific
Description: Designed to provide precise temperature stability and uniformity. The units have an easy to use touch screen control which displays time and temperature and are durable with a powder-coated steel body. Ideal for sample preparation, enzyme preservation, enzyme-substrate reactions, DNA amplification, electrophoresis gel degeneration and serum coagulation. Choice of models to hold 1, 2 and 4 interchangeable modular blocks to accommodate a variety of vessels and applications. Interchangeable aluminum alloy sample blocks designed to provide versatility, easy cleaning and disinfecting.

Product Image-PRSI36-170

Anti-VASP Mouse Monoclonal Antibody [clone: IE273]

Catalog Number: (PRSI36-170)
Supplier: ProSci Inc.
Description: VASP (vasodilator-stimulated phosphoprotein) is a proline-rich protein substrate of cAMP- and cGMP-dependent protein kinases. VASP is an actin-associated protein involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation. It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping proteins. VASP stimulates actin filament elongation by promoting the transfer of profilin-bound actin monomers onto the barbed end of growing actin filaments and it plays a role in actin-based mobility of Listeria monocytogenes in host cells. It regulates actin dynamics in platelets and plays an important role in regulating platelet aggregation. VASP phosphorylation is used to monitor the effect of so-called antiplatelet drugs that reduce platelet reactivity and are used to prevent stent thrombosis, strokes and heart attacks in patients. Phosphorylation of VASP at Ser157 causes a mobility shift in SDS gel electrophoresis from 46 to 50kDa, which has been used as a convenient marker to monitor cyclic nucleotide-dependent protein kinase activity.
UOM: 1 * 50 µG
Product Image-17-1323-01

Glycine, PlusOne™

Catalog Number: (17-1323-01)
Supplier: Cytiva
Description: Glycine is frequently used as a component of electrophoresis buffers, especially in the Laemmli discontinuous buffer system for SDS-PAGE.
UOM: 1 * 500 g

MSDS


Product Image-461-0883

Refrigerated/heating circulators, CORIO™ CD series

Supplier: JULABO GmbH
Description: These powerful and robust CORIO™ laboratory circulators provide the exact temperature, absolute precision and a wide working temperature range. The CORIO™ CD controller is an advanced unit with professional technology for demanding applications and can be used for temperature control of internal or external applications. The jet nozzle allows continuous adjustment of the pump stream in the system. The insulated stainless steel baths are durable and have an integrated drain tap. These heating/cooling circulators are ideal for temperature control of samples in a circulator bath or temperature control of an external application. For example: Measuring cells, refractometers, polarimeters, photometers, viscometers, fermenters, electrophoresis chambers, chromatography columns, rotary evaporators and rheometers.

Environmentally Preferable

Product Image-D2500-02

Gel extraction kits, E.Z.N.A.®

Supplier: OMEGA BIO-TEK
Description: Gel purification of DNA is a common technique used for the isolation of specific DNA fragments. However, most methods either fail to completely remove agarose (which can lead to problems in downstream manipulations), shear the DNA, or result in very low yields.

MSDS Certificates

Image Unavailable-HEWLG7100-60400

Alignment interfaces and cassettes

Catalog Number: (HEWLG7100-60400)
Supplier: Agilent
Description: These alignment interfaces are an integral part of the Agilent diode-array detection system.
UOM: 1 * 1 items
Image Unavailable-D6294-00

MicroElute® Cycle-Pure & Gel Extraction Kit, Omega Bio-tek

Supplier: OMEGA BIO-TEK
Description: MicroElute® Clean Up system, designed for rapid DNA clean up.

MSDS

Image Unavailable-730-1162

Gel analysis software, Dymension

Supplier: Syngene (a division of the Synoptics group)
Description: Dymension software can be used to analyse a typical 2D gel image rapidly. It features algorithms for background subtraction, noise filtering, precise alignment, spot detection, rapid matching and reduced image editing time. Using its powerful spot detection algorithm, Dymension instantly locates and analyses protein spots. With Dymension, the entire analysis process from background correction to spot matching results and reporting takes minutes. Dymension is available in three versions, and upgrading between the packages is simple.

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on +353 1 88 22222.
Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on +353 1 88 22222
This product is marked as restricted and can only be purchased by approved Shipping Accounts. If you need further assistance, email VWR Regulatory Department at eurega_services@eu.vwr.com
-Additional Documentation May be needed to purchase this item. A VWR representative will contact you if needed.
This product has been blocked by your organisation. Please contact your purchasing department for more information.
The original product is no longer available. The replacement shown is available.
Product(s) marked with this symbol are discontinued - sold till end of stock. Alternatives may be available by searching with the VWR Catalog Number listed above. If you need further assistance, please call VWR Customer Service on +353 1 8822222.
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