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Gram′s safranin solution for Gram staining, for microscopy, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Description: Gram’s safranin solution, also known as Safranin T or Basic Red 2, is a ready-to-use staining solution that comprises 10% safranin in ethanol. Safranin is a basic, cationic, azine dye containing a planar, diaminophenazine chromophore, with a phenyl substituent attached to a ring nitrogen. The trimethyl cationic form of safranine is lipophilic, whereas the dimethyl forms are weakly hydrophilic. It is most notably used in bacteriology as a counterstain in Gram staining to differentiate between Gram-positive and Gram-negative bacteria.

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Safranine O solution for Gram staining, Sigma-Aldrich®

Supplier: Merck

Description: Counterstain for Gram staining

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Gram color staining kit, Sigma-Aldrich®

Catalog Number: (1.11885.0001)

Supplier: Merck

Description: Gram-colour staining kit, containing 500 ml each of crystal violet solution, Lugol's solution stabilised, Safranin solution and 2×500 ml decolourisation solution.

UOM: 1 * 5 items

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Safranine O ≥85% (dye content), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Description: Safranin O is a cationic dye, which displays less significant metachromasia. It is used to detect any variations in articular disease. Safranin O binds specifically to polyanions. It detects the amount of proteoglycan present in cartilages. Safranin O is used as a marker for glycosaminoglycan depletion in osteoarthritis. Safranin is used as a counter stain in gram staining.

Gram color staining kit for tissue, Sigma-Aldrich®

Catalog Number: (HT90T-1KT)

Supplier: SIGMA ALDRICH MICROSCOPY

Description: Tissues are stained for Gram-positive organisms with crystal violet, mordanted in iodine, decolorized, stained for Gram-negative organisms with safranin O and then counterstained with tartrazine. For delineating Gram-positive and Gram-negative organisms

UOM: 1 * 1 KIT

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Safranine O, Certifiable Grade

Supplier: VWR Chemicals

Description: Part Fleming Triple stain, a chromosome stain prepared with light green SF and in Gram stain.

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Gram color staining kit for films, Sigma-Aldrich®

Catalog Number: (HT90A-1KT)

Supplier: SIGMA ALDRICH MICROSCOPY

Description: Films are stained for Gram-positive organisms with crystal violet, mordanted in iodine, decolorized and then stained for Gram-negative organisms with safranin O. For delineating Gram-positive and Gram-negative organisms in films.

UOM: 1 * 1 KIT

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Safranine O solution

Supplier: VWR Chemicals

Description: Safranine O solution

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Gram-Hücker R Kit To differentiate Gram-positive from Gram-negative Bacteria

Catalog Number: (720-0350)

Supplier: RAL

Description: Gram-Hücker staining is based on the same principle than the Gram-Nicolle staining. The structure of the bacterial walls and their permeability to alcohol help differentiate the Gram-negative bacteria from the Gram-positive ones. In this technique, Carbolic Gentian Violet is replaced by Crystal Violet Oxalate (that does not require filtration) and Ziehl-Carbolic Fuchsin by Safranin.

UOM: 1 * 1 KIT

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Gram's decolourising solution for Gram staining, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Description: Gram staining is one of the most significant staining techniques in microbiology. Gram′s decolouriser solution is a mixture of ethanol and acetone. Gram′s decolouriser solution is used in Gram staining procedure, after the iodine step to remove the crystal violet dye. The decolouriser dissolves the lipid layer from the gram-negative cells.

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