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Product Image-BE-104

DNA fingerprinting kit

Catalog Number: (BE-104)
Supplier: G-Biosciences
Description: This DNA fingerprinting kit allows students to carry out their own criminal investigation by comparing DNA samples collected from suspects to DNA collected at a pseudo-crime scene.
UOM: 1 * 1 KIT
Product Image-EDVO207

Experimentation kits, southern blot analysis

Catalog Number: (EDVO207)
Supplier: EDVOTEK EUROPE LTD
Description: This experiment introduces students to Southern blotting as a tool for DNA fingerprinting in a hypothetical paternity determination. DNA fragments are first separated by agarose gel electrophoresis, then transferred to a nylon membrane and finally visualised by staining.
UOM: 1 * 1 items

Automated plate washing for ELISA, cells and beads

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Product Image-BE-409

Experimentation kits, conservation of genetic information

Catalog Number: (BE-409)
Supplier: G-Biosciences
Description: In this lab activity, students learn to perform carefully controlled experiments to generate protein fragments using a proteolysis enzyme and then analyse the protein fragments by electrophoresis. By analysis of protein fragmentation patterns, i.e. protein fingerprints, students learn about protein sequence, structure, and their conservation. Students resolve a set of three functionally identical protein samples selected from throughout the animal kingdom; including human, bovine and sheep. After generating fingerprints by electrophoresis, students examine the protein fingerprint of each sample to determine the degree of conservation.
UOM: 1 * 1 KIT
Product Image-BE-408

Experimentation kits, protein fingerprinting

Catalog Number: (BE-408)
Supplier: G-Biosciences
Description: In this lab activity, students learn to perform simple protein isolation procedures to isolate the protein fingerprint from various fresh tissues. They will compare the protein fingerprints of four different tissues to understand that the function of a particular organ is due to the proteins that are localised to the specific organ. Also included in this kit are four dried protein samples (from mouse liver, brain, heart and lungs) to compare as a control, if fresh tissues cannot be obtained.
UOM: 1 * 1 KIT
Product Image-EDVO371

Experimentation kits, DNA fingerprinting - using PCR

Catalog Number: (EDVO371)
Supplier: EDVOTEK EUROPE LTD
Description: Students can solve a crime using PCR. Plasmid DNA is provided that, when amplified by PCR, provides products that represent individual DNA profiles.
UOM: 1 * 1 KIT

MSDS


Product Image-EDVO124

Kits: DNA-based screening for smallpox, Ready-to-Load™

Catalog Number: (EDVO124)
Supplier: EDVOTEK EUROPE LTD
Description: This experiment presents a bioterrorism scenario, with students examining a simulated DNA fingerprinting test for the detection of smallpox.
UOM: 1 * 1 items
Product Image-EDVO114-C

Kits: DNA paternity test, Ready-to-Load™

Supplier: EDVOTEK EUROPE LTD
Description: This experiment introduces students to the use of DNA fingerprinting in a simulated paternity determination. A child's DNA fingerprint is compared with his parents.

Product Image-EDVO334

Experimentation kits, PCR-based VNTR human DNA typing

Catalog Number: (EDVO334)
Supplier: EDVOTEK EUROPE LTD
Description: In DNA fingerprinting, variable number tandem repeats (VNTR) are used to identify individuals. Using this kit, students type themselves at the D1S80 locus on chromosome 1. This region contains between 14 and 40 copies of a 16 base pair repeat.
UOM: 1 * 1 KIT
Product Image-EDVO225

Experimentation kits, DNA fingerprinting using restriction enzymes

Catalog Number: (EDVO225)
Supplier: EDVOTEK EUROPE LTD
Description: This experiment helps teach students about restriction enzyme digests in the context of forensic science. Students cut DNA with restriction enzymes and then compare the banding pattern of the crime scene DNA versus that of two suspects using agarose gel electrophoresis.
UOM: 1 * 1 KIT

MSDS


Product Image-BE-502

Experimentation kits, dot blot analysis

Catalog Number: (BE-502)
Supplier: G-Biosciences
Description: Dot blotting is a simple technique to identify a known protein in a biological sample. The ease and simplicity of the technique makes dot blotting an ideal diagnostic tool.
UOM: 1 * 1 KIT
Product Image-BE-401

Experimentation kits, physical properties of proteins

Catalog Number: (BE-401)
Supplier: G-Biosciences
Description: The physical properties of proteins kit is a lab activity that enables students to investigate the physical properties of several different proteins. Students will learn about protein solubility and how it is affected by various parameters; including temperature, pH, salt and dielectric constant. They will understand about protein precipitation due to pH, high salt and in the presence of organic solvents and about protein denaturation as a result of high temperature. In addition, the kit will demonstrate how non-protein agents, such as detergents drastically alter the physical properties of protein molecules and as a result, understand the importance of detergents in protein solubilisation. This lab activity involves analysis of three different types of pure proteins and then students alter some of those properties with a detergent and re-examine physical properties of those proteins. Students are challenged to consider how physical properties of protein molecules can be exploited for purification and characterisation of proteins and apply their findings on a test sample of complex tissue extract.
UOM: 1 * 1 KIT
Image Unavailable-730-2832

Data storage software, GeneDirectory

Catalog Number: (730-2832)
Supplier: Syngene (a division of the Synoptics group)
Description: GeneDirectory is a fast data storage and retrieval system designed for large-scale genomic fingerprinting studies, cluster analysis, VNTR analysis, genotyping and RFLP. Used in conjunction with the GeneTools gel analysis package, GeneDirectory can save hours of repetitive work by automatically comparing thousands of bands on different gels.
UOM: 1 * 1 items
Product Image-EDVO986

Experimentation kits, analysis and comparision of mammalian cell types

Catalog Number: (EDVO986)
Supplier: EDVOTEK EUROPE LTD
Description: Observe the differences between mammalian cell types and how these cells funciton. Cells are fixed on microscope slides and students stain the cells on the slide to view morphological characteristics of the cell types. These cells are very safe for classroom use.
UOM: 1 * 1 items
Image Unavailable-ENZOADIKAPCA002F

Anti-CAMK2 Rabbit Polyclonal Antibody

Supplier: ENZO LIFE SCIENCES AG
Description: CaMKII (calmodulin-dependent kinase II) is an enzyme which is activated by increases in intracellular Ca2+ ion concentration and it has been proposed to be pivotal in regulating synaptic strength and maturation of synapses during development.  This process is thought to be critical in memory and learning and in establishing the specificity of synaptic connections. There are over two dozen alternative splice variants of CaMKII which are encoded by four genes, a, b, g, and d with apparent molecular masses of 50-60 kDa.  CaMKII is widely distributed in many tissues, but is highly expressed in brain. Several studies demonstrate that CaMKII is a multifunctional enzyme which modulates the synaptic strength by binding to a subunit of NMDA receptors and promoting the phosphorylation of this NMDA receptor subunit.  CaMKII also regulates DLG localization at synapses by co-localization with DLG in the same protein complex.  Experimental data suggest that CaMKII is critically involved in the development of morphine tolerance as well as dependence and inhibition of this enzyme may have some therapeutic benefit in the treatment of opiate tolerance and dependence.  It also has been demonstrated that d CaMKII isozyme is down-regulated in human tumor cells indicating a role for d CaMKII isozymes in cellular differentiation.  Changes in d CaMKII isozyme expression pattern in human hearts during heart failure suggest that CaMKII is important for regulation of heart function.  This immunoaffinity purified antibody detects proteins of 50-60 kDa, corresponding to apparent molecular mass of CaMKII isoforms on SDS-PAGE immunoblots, in samples from human, mouse, rat, bovine, hamster, guinea pig, chicken and rabbit origins. Recombinant rat calmodulin-dependent protein kinase II a subunits expressed in sf9 insect cells are also detected. As the sequences of the rat a, d, and g isoforms are conserved over this amino terminus region, this antibody is expected to recognize the d, g and a isoforms. This antibody is not expected to cross-react with the b isoform. Proteins of unknown identity, ~40, 45 and 90 kDa, may be detected on immunoblot analysis with some lysates.

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Product Image-EDVO332

Experimentation kits, mitochondrial DNA analysis using PCR

Catalog Number: (EDVO332)
Supplier: EDVOTEK EUROPE LTD
Description: The mitochondria are thought to have evolved from a symbiotic relationship between prokaryotic and eukaryotic cells. Mitochondria have their own DNA and are only inherited via the maternal line. In this experiment, students amplify two regions of their mitochondrial DNA.
UOM: 1 * 1 KIT
Product Image-EDVO112-C

Kits: analysis of Eco RI cleavage patterns of lambda DNA, Ready-to-Load™

Supplier: EDVOTEK EUROPE LTD
Description: Ready-to-Load™ kit for introducing the use of restriction enzymes as a tool to digest lambda DNA at specific nucleotide sequences.

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on +353 1 88 22222
This product is marked as restricted and can only be purchased by approved Shipping Accounts. If you need further assistance, email VWR Regulatory Department at eurega_services@eu.vwr.com
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