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Product Image-786-226

Agarose Sealing Solution

Catalog Number: (786-226)
Supplier: G-Biosciences
Description: Simply heat and use for sealing the IPG strips for SDS-PAGE analysis. The Agarose Sealing Solution is prepared in a proprietary buffer to minimise reoxidation of the competing thiol pairs as proteins enter into the second dimension gel. Improves resolution and prevents streaking of protein spots on 2D gels.
UOM: 1 * 50 mL
Image Unavailable-ICNA04808831

Glycine ≥99.5%

Supplier: MP Biomedicals
Description: Electrophoresis Grade Heavy metals (as Pb): 20 ppm Used for preparation of Tris-glycine buffers.

Image Unavailable-EKSIC44807

Low pH SDS Sample Buffer

Catalog Number: (EKSIC44807)
Supplier: EKSIGENT
Description: SCIEX Low pH SDS Sample Buffer, 140 mL (100 mM Tris-HCl, pH 6.8, 1% SDS) allows you to use SCIEX’s Gold Standard CE-SDS with a low pH SDS sample buffer. Use of this buffer can increase non-reduced purity by decreasing method induced fragmentation. Less variability and lower RSDs means less troubleshooting and faster method development.
UOM: 1 * 1 items
Image Unavailable-HEWLG1607-60041

CE & CE/MS Supplies, Agilent Technologies

Supplier: Agilent
Description: CE/MS sprayer kit and CE/MS adapter kit to couple an Agilent CE instrument with an MS system.

Product Image-PIER89888

Pierce™ SDS-PAGE Sample Prep Kit

Catalog Number: (PIER89888)
Supplier: Thermo Fisher Scientific
Description: Pierce SDS-PAGE sample prep kit quickly and easily removes high levels of salts, denaturants, detergents and other buffer components that interfere with polyacrylamide gel electrophoresis of proteins.
UOM: 1 * 1 KIT
Product Image-17-1321-01

Tris(hydroxymethyl)aminomethane (TRIS, Trometamol)

Catalog Number: (17-1321-01)
Supplier: Cytiva
Description: Tris (tris[hydroxymethyl] aminoethane) is suitable for preparing electrophoresis buffer in the pH range of 7,2 to 9,0. It is qualified for 2-D electrophoresis.
UOM: 1 * 500 g

MSDS


Image Unavailable-ICNA0210112325

Bromochlorophenol blue, powder

Supplier: MP Biomedicals
Description: Bromophenol blue is a tracking dye for alkaline and neutral buffer systems. It is used as a tracking dye in DNA, RNA (agarose) and protein (polyacrylamide) gel electrophoresis.

Product Image-786-411

Protein extraction kit, Insect PE LB™

Catalog Number: (786-411)
Supplier: G-Biosciences
Description: Insect PE LB™ has been developed for extraction of total biologically active, soluble proteins from adherent or suspension cultured insect cells, including Sf9 and Sf21. Insect PE LB™ utilises a mild non-ionic detergent and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. The Insect PE LB™ is fully compatible with downstream processes, such as electrophoresis and chromatography. Depending on the required downstream application, additional agents such as reducing agents and protease inhibitors may be added into Insect PE LB™.
UOM: 1 * 250 mL
Image Unavailable-17-6003-18

Rehydration reagent, DeStreak

Supplier: Cytiva
Description: The appearance of streaks that distort 2-D electrophoresis maps is a common problem, occurring most frequently when running gels that contain regions greater than pH 7,0. Increased sample load, increased length of the IPG strip, or using a narrower pH gradient worsens the problem. Extra spots on 2-D gels, caused by non specific oxidation of proteins, is another difficulty encountered when running gels containing basic regions. Both streaking and non specific oxidation result in poorly resolved protein patterns and reduced reproducibility between electrophoresis runs.

Image Unavailable-ICNA04805732

Bromophenol blue, powder

Catalog Number: (ICNA04805732)
Supplier: MP Biomedicals
Description: Bromophenol blue is a tracking dye for alkaline and neutral buffer systems. It is used as a tracking dye in DNA, RNA (agarose) and protein (polyacrylamide) gel electrophoresis.
UOM: 1 * 10 g

Certificates


Product Image-28-9374-51

Precast gels, DIGE Gel

Catalog Number: (28-9374-51)
Supplier: Cytiva
Description: DIGE gel is a 12,5% precast polyacrylamide gel in a low fluorescent glass cassette for second dimension 2-D electrophoresis and specially developed for 2-D DIGE analysis. DIGE gel and DIGE buffer kit are tools to increase the quality, reproducibility, and convenience of 2-D DIGE using Ettan™ DIGE system.
UOM: 1 * 3 items
Product Image-BE-406

Experimentation kits, protein electrophoresis

Catalog Number: (BE-406)
Supplier: G-Biosciences
Description: Using the Protein Electrophoresis kit, students will learn the principles of various types of electrophoresis, including denaturing and non-denaturing electrophoresis, and how this powerful technique is used to analyse proteins. The kit will introduce students to the different separation matrices currently in use and will understand their differing separation properties and their role in protein analysis. Students have an option of casting their own electrophoresis gels using polyacrylamide or using pre-cast commercially available gels. This kit is provided with all of the reagents, buffers and supplies needed for casting acrylamide gels, preparing protein samples, running electrophoresis, and staining the gels for visualisation of protein bands. Test protein samples and protein standards are also provided.
UOM: 1 * 1 KIT
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Sodium acetate, anhydrous ≥99.0%, crystalline powder ACS

Supplier: MP Biomedicals
Description: Sodium acetate is used as a buffer in the buffering range of pH 3.6-5.6. It is also used in the purification and precipitation of nucleic acids, protein crystallization, staining of gels in protein gel electrophoresis, HPLC, mordant dyeing, DNA microarray studies of E. coli response, investigation of protein unfolding during reverse phase chromatography.

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Polyvinyl alcohol (PVA) coated capillaries

Supplier: Agilent
Description: PVA coated capillaries contain a permanently adsorbed layer of polyvinyl alcohol. This coating minimises hydrophobic and electrostatic solute/wall interactions and eliminates electroosmotic flow (EOF). Using a proprietary deposition process, the PVA coating is stable over a wide pHrange, even under basic conditions from 2,5 to 9,5. This stability allows the use of many common CE buffers. Because the silica surface is covered, many proteins and amines can be analysed without the peak tailing found with uncoated capillaries. In addition, since EOF is eliminated, cumbersome washing procedures are unnecessary and migration time reproducibility may be improved.

Image Unavailable-ICNA02103130.1

TRIS HCl (tris(hydroxymethyl)aminomethane hydrochloride) ≥99%, Reagent Grade

Supplier: MP Biomedicals
Description: Tris and Tris Hydrochloride have been useful as buffers in a wide variety of biological systems. Uses include pH control<i> in vitro </i>and <i>in vivo</i> for body fluids and in buffering systems for electrophoresis applications.

Product Image-733-1618

FlashGel™ RNA system

Supplier: LONZA
Description: FlashGel™ system for RNA is optimised for the unique requirements of RNA, and is an ideal tool for rapid analysis of sample integrity. High quality, intact RNA is essential for consistent results in gene expression, Northern analysis, cDNA library construction and cDNA labelling for microarrays. Most protocols recommend checking RNA prior to downstream analysis.

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on +353 1 88 22222
This product is marked as restricted and can only be purchased by approved Shipping Accounts. If you need further assistance, email VWR Regulatory Department at eurega_services@eu.vwr.com
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