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Product Image-786-161

Wash buffers, femto TBST™ and femto PBST™

Supplier: G-Biosciences
Description: The femto TBST™ and femto PBST™ wash buffers will enhance sensitivity of immunoassays by minimising the 'washing out' of immuno agents or immuno complexes, a common problem associated with classical TBST and PBST buffers. The wash buffers aid in the generation of cleaner backgrounds, resulting in a larger ratio of signal to background. These can be used for incubation and washing procedures.

Product Image-PIER89888

Pierce™ SDS-PAGE Sample Prep Kit

Catalog Number: (PIER89888)
Supplier: Thermo Fisher Scientific
Description: Pierce SDS-PAGE sample prep kit quickly and easily removes high levels of salts, denaturants, detergents and other buffer components that interfere with polyacrylamide gel electrophoresis of proteins.
UOM: 1 * 1 KIT
Product Image-730-0183

Mini horizontal electrophoresis system, Owl™ B1A EasyCast™

Catalog Number: (730-0183)
Supplier: THERMO OWL SCIENTIFIC
Description: The Owl™ B1A EasyCast™ mini gel system is designed to provide flat, even banding patterns and consistent results with minimum effort. This system is ideal for determination of molecular weight ranges of PCR fragments or larger sized DNA molecules. The all-in-one design allows the option of casting and running a gel in the buffer chamber, eliminating the need for additional equipment.
UOM: 1 * 1 items
Image Unavailable-EKSIC44807

Low pH SDS Sample Buffer

Catalog Number: (EKSIC44807)
Supplier: EKSIGENT
Description: SCIEX Low pH SDS Sample Buffer, 140 mL (100 mM Tris-HCl, pH 6.8, 1% SDS) allows you to use SCIEX’s Gold Standard CE-SDS with a low pH SDS sample buffer. Use of this buffer can increase non-reduced purity by decreasing method induced fragmentation. Less variability and lower RSDs means less troubleshooting and faster method development.
UOM: 1 * 1 items
Product Image-17-1321-01

Tris(hydroxymethyl)aminomethane (TRIS, Trometamol)

Catalog Number: (17-1321-01)
Supplier: Cytiva
Description: Tris (tris[hydroxymethyl] aminoethane) is suitable for preparing electrophoresis buffer in the pH range of 7,2 to 9,0. It is qualified for 2-D electrophoresis.
UOM: 1 * 500 g

MSDS


Product Image-PIER78437

Protease inhibitor cocktail, Halt

Supplier: Thermo Fisher Scientific
Description: Halt Protease Inhibitor Cocktail provides all the protection of a broad-spectrum protease inhibitor in a single, ready-to-use liquid format. Halt protects against protein degradation by serine, cysteine, and aspartic acid proteases and aminopeptidases. Metalloproteases are inhibited by the optional use of 0,5 M EDTA. The 100X Halt stock solution makes it easy to dispense the required amount (10 µl per 1 ml) of protease inhibitors into buffers or lysis reagents prior to extraction of proteins from cultured cells, animal tissues, plant tissues, yeast or bacteria. Halt Protease Inhibitor Cocktails are compatible with Thermo Scientific Pierce Protein Extraction Reagents. Halt EDTA-free formulation is ideal for preparing samples that will be analysed by 2-D gel electrophoresis, immobilised metal chelate affinity chromatography (IMAC) and other downstream application where chelating agents interfere.

Product Image-28-9374-51

Precast gels, DIGE Gel

Catalog Number: (28-9374-51)
Supplier: Cytiva
Description: DIGE gel is a 12,5% precast polyacrylamide gel in a low fluorescent glass cassette for second dimension 2-D electrophoresis and specially developed for 2-D DIGE analysis. DIGE gel and DIGE buffer kit are tools to increase the quality, reproducibility, and convenience of 2-D DIGE using Ettan™ DIGE system.
UOM: 1 * 3 items
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Bromochlorophenol blue, powder

Supplier: MP Biomedicals
Description: Bromophenol blue is a tracking dye for alkaline and neutral buffer systems. It is used as a tracking dye in DNA, RNA (agarose) and protein (polyacrylamide) gel electrophoresis.

Product Image-786-411

Protein extraction kit, Insect PE LB™

Catalog Number: (786-411)
Supplier: G-Biosciences
Description: Insect PE LB™ has been developed for extraction of total biologically active, soluble proteins from adherent or suspension cultured insect cells, including Sf9 and Sf21. Insect PE LB™ utilises a mild non-ionic detergent and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. The Insect PE LB™ is fully compatible with downstream processes, such as electrophoresis and chromatography. Depending on the required downstream application, additional agents such as reducing agents and protease inhibitors may be added into Insect PE LB™.
UOM: 1 * 250 mL
Image Unavailable-17-6003-18

Rehydration reagent, DeStreak

Supplier: Cytiva
Description: The appearance of streaks that distort 2-D electrophoresis maps is a common problem, occurring most frequently when running gels that contain regions greater than pH 7,0. Increased sample load, increased length of the IPG strip, or using a narrower pH gradient worsens the problem. Extra spots on 2-D gels, caused by non specific oxidation of proteins, is another difficulty encountered when running gels containing basic regions. Both streaking and non specific oxidation result in poorly resolved protein patterns and reduced reproducibility between electrophoresis runs.

Image Unavailable-ICNA04805732

Bromophenol blue, powder

Catalog Number: (ICNA04805732)
Supplier: MP Biomedicals
Description: Bromophenol blue is a tracking dye for alkaline and neutral buffer systems. It is used as a tracking dye in DNA, RNA (agarose) and protein (polyacrylamide) gel electrophoresis.
UOM: 1 * 10 g

Certificates


Image Unavailable-ICNA0219549601

Sodium acetate, anhydrous ≥99.0%, crystalline powder ACS

Supplier: MP Biomedicals
Description: Sodium acetate is used as a buffer in the buffering range of pH 3.6-5.6. It is also used in the purification and precipitation of nucleic acids, protein crystallization, staining of gels in protein gel electrophoresis, HPLC, mordant dyeing, DNA microarray studies of E. coli response, investigation of protein unfolding during reverse phase chromatography.

Product Image-BE-406

Experimentation kits, protein electrophoresis

Catalog Number: (BE-406)
Supplier: G-Biosciences
Description: Using the Protein Electrophoresis kit, students will learn the principles of various types of electrophoresis, including denaturing and non-denaturing electrophoresis, and how this powerful technique is used to analyse proteins. The kit will introduce students to the different separation matrices currently in use and will understand their differing separation properties and their role in protein analysis. Students have an option of casting their own electrophoresis gels using polyacrylamide or using pre-cast commercially available gels. This kit is provided with all of the reagents, buffers and supplies needed for casting acrylamide gels, preparing protein samples, running electrophoresis, and staining the gels for visualisation of protein bands. Test protein samples and protein standards are also provided.
UOM: 1 * 1 KIT
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Polyvinyl alcohol (PVA) coated capillaries

Supplier: Agilent
Description: PVA coated capillaries contain a permanently adsorbed layer of polyvinyl alcohol. This coating minimises hydrophobic and electrostatic solute/wall interactions and eliminates electroosmotic flow (EOF). Using a proprietary deposition process, the PVA coating is stable over a wide pHrange, even under basic conditions from 2,5 to 9,5. This stability allows the use of many common CE buffers. Because the silica surface is covered, many proteins and amines can be analysed without the peak tailing found with uncoated capillaries. In addition, since EOF is eliminated, cumbersome washing procedures are unnecessary and migration time reproducibility may be improved.

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Glycine ≥99% for molecular biology

Supplier: MP Biomedicals
Description: Storage: Store at Room Temperature (15-30 °C)
Glycine is a non-essential amino acid. It is only amino acid with no asymmetric carbon and thus is not chiral. It is the major inhibitory neurotransmitter. It is involved in the biosynthesis of the porphyrin rings of hemes and chlorophylls.
Glycine is commonly used in buffer solutions, in electrophoresis and preparative chromatography. A study of the folding of monoclonal antibodies in the presence of glycine and their subsequent purification has been published. The use of glycine in the purification of lipopolysaccharides, lipooligosaccharides, and lipid A has been reported. It is an amino acid for use in cell culture media development applications and existing media formulations. Glycine is commonly used as a component in Tris-glycine and Tris-glycine-SDS running buffers for polyacrylamide gel electrophoresis, a component of Towbin's transfer buffer for Western blots, a buffer substance in cryoenzymology, in osmotic pressure maintenance in isoelectric focusing of erythrocytes, salting-in effect in protein chemistry, and as a buffer component in the coupled phosphatase-kinase reaction for end labelling of restriction fragments. The growth requirements of various microorganisms is reported in the Handbook of Microbiology.
Glycine is a non-chiral amino acid that can be synthesized in the body from the amino acid serine by Serine Hydroxymethyltransferase. Inhibitory neurotransmitter in spinal cord, allosteric regulator of NMDA receptors.

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TRIS HCl (tris(hydroxymethyl)aminomethane hydrochloride) ≥99%, Reagent Grade

Supplier: MP Biomedicals
Description: Tris and Tris Hydrochloride have been useful as buffers in a wide variety of biological systems. Uses include pH control<i> in vitro </i>and <i>in vivo</i> for body fluids and in buffering systems for electrophoresis applications.

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on +353 1 88 22222
This product is marked as restricted and can only be purchased by approved Shipping Accounts. If you need further assistance, email VWR Regulatory Department at eurega_services@eu.vwr.com
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The original product is no longer available. The replacement shown is available.
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