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Density gradient medium, OptiPrep™

Catalog Number: (D1556-250ML)
Supplier: SIGMA-ALDRICH MICROSCOPY
Description: Density gradient medium is suitable for cell and subcellular organelle isolation, virus purification, and isolation of lipoproteins and macromolecules.
UOM: 1 * 250 mL
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Percoll®, Siam

Supplier: SIGMA-ALDRICH MICROSCOPY
Description: Percoll® is a classic medium for density gradient centrifugation of cells, viruses, and subcellular particles. It consists of colloidal silica particles of 15 – 30 nm diameter (23% w/w in water), coated with polyvinylpyrrolidone (PVP).

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Bürkle
Product Image-E965-50G

VWR® Ficoll® 400 Biotechnology Grade

Supplier: VWR Chemicals
Description: Ficoll® 400 is a neutral, hydrophilic polymer of sucrose, which readily dissolves in aqueous solutions.

MSDS

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VWR®, Lymphocyte separation media

Supplier: VWR Chemicals
Description: Lymphocyte separation media is a density gradient medium for the isolation of mononuclear cells from peripheral blood, cord blood and bone marrow.

MSDS Certificates

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Lymphocyte separation media, LymphoSep™

Supplier: Biowest
Description: Lymphosep is a standardised, high quality medium designed for simple and rapid isolation of PBMCs from treated whole blood. Lymphosep, like Ficoll™, has the optimised density 1,077 g/ml for lymphocyte separation to guarantee best and reliable results for your preparations.

Certificates

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VWR®, Trypsin-EDTA solutions

Supplier: VWR Chemicals
Description: VWR® Trypsin-EDTA solutions are used in cell culture to remove adherent cells from a culture surface. Trypsin is a porcine pancreas-derived enzyme that is commonly used for the dissociation and disaggregation of anchorage-dependent mammalian cells and tissues. The concentration of trypsin necessary to separate cells from their substrate is dependent primarily on the cell type and the age of the culture. EDTA, a chelating agent, enhances enzymatic activity by removing calcium and magnesium ions. These ions obscure the peptide bonds on which trypsin acts as well as enhancing cell to cell adhesion.

MSDS Certificates

Product Image-78444.

Halt™ Protease and Phosphatase Inhibitor Cocktail

Supplier: Thermo Fisher Scientific
Description: Halt protease and phosphatase inhibitor cocktail provides the convenience of a single solution with full sample protection. This broad-spectrum inhibitor cocktail is specifically optimised to protect proteins from degradation during extraction and purification. The cocktail contains inhibitors against the major classes of proteases and phosphatases, targeting aminopeptidases, cysteine and serine proteases and serine/threonine and protein tyrosine phosphatases.

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Phytohaemagglutinin-M (PHA-M)

Catalog Number: (L3010-005)
Supplier: Biowest
Description: Phytohaemagglutinin is a lectin extracted from red kidney beans (<i>Phaseolus vulgaris</i>). PHA-M is the mucoprotein form and is a crude extract used for the stimulation of cell proliferation in lymphocyte culture. PHA-M also has a powerful erythroagglutinating property and it was originally used for separating leukocytes from whole blood.
UOM: 1 * 5 mL
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Percoll™ PLUS

Supplier: Cytiva
Description: Percoll® PLUS is for preparation of cells, subcellular particles and larger viruses.

MSDS Certificates

Product Image-734-1433

Corning® BioCoat™ Intestinal Epithelium Differentiation Media Pack, Corning

Catalog Number: (734-1433)
Supplier: Corning
Description: Corning® BioCoat™ Intestinal Epithelium Differentiation Environment is an integrated system designed to create <i>in vitro</i> intestinal models.
UOM: 1 * 1 KIT

Certificates


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Ficoll® PM400

Supplier: Cytiva
Description: Ficoll® PM400 is a synthetic neutral, highly branched hydrophilic polymer of sucrose with an average molecular weight of 400000. It is used to form density gradients for separating and isolating eukaryotic cells, organelles and bacterial cells, as a stabilising agent and as a preparation medium for isolating mononuclear cells. It is useful for separating cells that are sensitive to centrifugation. Ficoll™ PM400 can also be used in other applications, such in defined culture media, nucleic acid hybridisation, electrophoresis, and immunological studies.

Certificates

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Ficoll® 400

Supplier: Thermo Scientific
Description: Cell separation media, Ficoll® 400

MSDS

Product Image-17-0891-01

Percoll™

Supplier: Cytiva
Description: Percoll® is commonly used for separating cells, organelles, viruses, and other subcellular particles.

MSDS Certificates

Product Image-734-1431

Intestinal Epithelium differentiation medium, Corning®

Catalog Number: (734-1431)
Supplier: Corning
Description: Intestinal Epithelium Differentiation Medium (formerly Entero-STIM) promotes the rapid differentiation of intestinal epithelium <i>in vitro</i>.
UOM: 1 * 500 mL
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Lymphocyte separation medium, LymphoSep™, MP Biomedicals

Supplier: MP Biomedicals
Description: Lymphocyte separation medium, LYMPHOSEP® is a sterile, iso-osmotic, low viscosity solution for density centrifugation yielding a clear separation of lymphocytes from heparinised or defibrinated human blood.

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Ficoll® 400, molecular biology grade

Supplier: Merck Millipore (Calbiochem‎)
Description: Ficoll® 400 is useful for the separation of lymphocyte sub populations. DNases, proteases, RNases: none detected.

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on +353 1 88 22222.
Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on +353 1 88 22222
This product is marked as restricted and can only be purchased by approved Shipping Accounts. If you need further assistance, email VWR Regulatory Department at eurega_services@eu.vwr.com
-Additional Documentation May be needed to purchase this item. A VWR representative will contact you if needed.
This product has been blocked by your organisation. Please contact your purchasing department for more information.
The original product is no longer available. The replacement shown is available.
Product(s) marked with this symbol are discontinued - sold till end of stock. Alternatives may be available by searching with the VWR Catalog Number listed above. If you need further assistance, please call VWR Customer Service on +353 1 8822222.
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