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Supplier: Thermo Fisher Scientific
Description: Aluminium lanthanum (III) oxide substrate 10x10x0.5 mm, polished one side 100 orientation
Catalog Number: (BOSSBS-1135R-A350)
Supplier: Bioss
Description: c-Src tyrosine kinase plays a critical role in signal transduction downstream of growth factor receptors, integrins and G protein-coupled receptors. We used stable isotope labeling with amino acids in cell culture (SILAC) approach to identify additional substrates of c-Src tyrosine kinase in human embryonic kidney 293T cells. We have identified 10 known substrates and interactors of c-Src and Src family kinases along with 26 novel substrates. We have experimentally validated 4 of the novel proteins (NICE-4, RNA binding motif 10, FUSE-binding protein 1 and TRK-fused gene) as direct substrates of c-Src using in vitro kinase assays and cotransfection experiments. Significantly, using a c-Src specific inhibitor, we were also able to implicate 3 novel substrates (RNA binding motif 10, EWS1 and Bcl-2 associated transcription factor) in PDGF signaling. Finally, to identify the exact tyrosine residues that are phosphorylated by c-Src on the novel c-Src substrates, we designed custom peptide microarrays containing all possible tyrosine-containing peptides (312 unique peptides) and their mutant counterparts containing a Tyr -->Phe substitution from 14 of the identified substrates. Using this platform, we identified 34 peptides that are phosphorylated by c-Src. We have demonstrated that SILAC-based quantitative proteomics approach is suitable for identification of substrates of nonreceptor tyrosine kinases and can be coupled with peptide microarrays for high-throughput identification of substrate phosphopeptides.
UOM: 1 * 100 µl


Catalog Number: (38500.KT)
Supplier: Thermo Fisher Scientific
Description: Aluminium oxide substrate 10x10x0.5 mm, polished one side R plane
UOM: 1 * 1 items

Supplier: Thermo Fisher Scientific
Description: Aluminium oxide substrate 10x10x1 mm, polished one side C plane
Catalog Number: (ENZOBMLP3160500)
Supplier: ENZO LIFE SCIENCES
Description: PTP substrate
UOM: 1 * 0,5 mg


Catalog Number: (ENZOBMLP3030005)
Supplier: ENZO LIFE SCIENCES
Description: Proteinase substrate
UOM: 1 * 5 mg


Catalog Number: (42594.KT)
Supplier: Thermo Fisher Scientific
Description: Strontium titanate substrate 5x5x0.5 mm, polished both sides 111 orientation
UOM: 1 * 1 items

Catalog Number: (38507.KT)
Supplier: Thermo Fisher Scientific
Description: Strontium titanate substrate 10x10x1 mm, polished one side 100 orientation
UOM: 1 * 1 items

Catalog Number: (ENZOBMLP2310001)
Supplier: ENZO LIFE SCIENCES
Description: AMPK substrate
UOM: 1 * 1 mg


Catalog Number: (USBIC2580-01)
Supplier: US Biological
Description: Anti-CDKs Substrate Rabbit Monoclonal Antibody [clone: 13G10]
UOM: 1 * 100 µl


Catalog Number: (ENZOBMLP8010005)
Supplier: ENZO LIFE SCIENCES
Description: Substrate for ubiquitin C-terminal hydrolases (UCHs)
UOM: 1 * 5 mg


Supplier: ENZO LIFE SCIENCES
Description: PLA₂ substrate

Catalog Number: (ENZOALX260048M001)
Supplier: ENZO LIFE SCIENCES
Description: Caspase substrate
UOM: 1 * 1 mg


Catalog Number: (ENZOBMLP2510100)
Supplier: ENZO LIFE SCIENCES
Description: Protein kinase substrate
UOM: 1 * 100 µG


Catalog Number: (ENZOBMLP2490001)
Supplier: ENZO LIFE SCIENCES
Description: Protein kinase substrate
UOM: 1 * 1 mg


Supplier: ENZO LIFE SCIENCES
Description: Caspase-2 substrate

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on +353 1 88 22222.
Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on +353 1 88 22222
This product is marked as restricted and can only be purchased by approved Shipping Accounts. If you need further assistance, email VWR Regulatory Department at eurega_services@eu.vwr.com
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This product has been blocked by your organisation. Please contact your purchasing department for more information.
The original product is no longer available. The replacement shown is available.
Product(s) marked with this symbol are discontinued - sold till end of stock. Alternatives may be available by searching with the VWR Catalog Number listed above. If you need further assistance, please call VWR Customer Service on +353 1 8822222.
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