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Image Unavailable-PIERA35395

Sulpho-SANPAH (Sulphosuccinimidyl-6-(4′-azido-2′-nitrophenylamino)hexanoate), No-Weigh™ Format, Pierce™

Catalog Number: (PIERA35395)
Supplier: Thermo Fisher Scientific
Description: Thermo Scientific Pierce Sulfo-SANPAH is a hetero-bifunctional crosslinker that contains an amine-reactive N-hydroxysuccinimide (NHS) ester and a photoactivatable nitrophenyl azide.
UOM: 1 * 10 mg
Product Image-PIER22317

Sulpho-SMPB (Sulphosuccinimidyl 4-(4-maleimidophenyl)butyrate), Pierce™

Catalog Number: (PIER22317)
Supplier: Thermo Fisher Scientific
Description: Thermo Scientific Pierce Sulfo-SMPB is a water-soluble amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a medium-length aromatic spacer arm (11.6 angstroms).
UOM: 1 * 50 mg
Product Image-PIER22362

LC-SMCC (N-Succinimidyl 6-[[4-(N-Maleimidomethyl)cyclohexyl]carboxamido]hexanoate), Pierce™

Catalog Number: (PIER22362)
Supplier: Thermo Fisher Scientific
Description: Thermo Scientific Pierce LC-SMCC is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a long cyclohexane-stabilized spacer arm (16.2 angstroms).
UOM: 1 * 50 mg
Product Image-PIER46604

PEGylated dyes, DyLight™

Supplier: Thermo Fisher Scientific
Description: DyLight™ PEGylated dyes display absorption spectra ranging from 550 nm to 783 nm. These reagents fluoresce over a broad pH range, are more intense than Alexa Fluor® or Cy® dyes in many applications and match the output wavelengths of common fluorescence instrumentation. Additionally, the water solubility of the DyLight™ reagents allows a high dye-to-protein ratio without precipitation during conjugation.

Product Image-AATB217

6-NED azide fluorescent probe

Catalog Number: (AATB217)
Supplier: AAT BIOQUEST
Description: 5'-Labeled primer pairs include a fluorescently labeled oligo with a choice of dye on the 5' end, along with an unlabeled oligo, in each pair.
UOM: 1 * 1 mg
Product Image-PIER21558

SMPT (4-succinimidyloxycarbonyl-alpha-methyl-α(2-pyridyldithio)toluene), Pierce™

Catalog Number: (PIER21558)
Supplier: Thermo Fisher Scientific
Description: Thermo Scientific Pierce SMPT is a long-chain crosslinker for amine-to-sulfhydryl conjugation via NHS-ester and pyridyldithiol reactive groups that form strong (hindered) yet cleavable (reducible) disulfide bonds with cysteine sulfhydryls..
UOM: 1 * 50 mg
Product Image-PIER21650

Sulpho-LC-SPDP (Sulphosuccinimidyl 6-(3'-(2-pyridyldithio)propionamido)hexanoate) ≥90%, Pierce™

Catalog Number: (PIER21650)
Supplier: Thermo Fisher Scientific
Description: Thermo Scientific Pierce Sulfo-LC-SPDP is a water-soluble, long-chain crosslinker for amine-to-sulfhydryl conjugation via NHS-ester and pyridyldithiol reactive groups that form cleavable (reducible) disulfide bonds with cysteines.
UOM: 1 * 50 mg
Product Image-AATB4501

SMCC (N-Succinimidyl 4-(N-Maleimidomethyl)cyclohexanecarboxylate) ≥85%

Supplier: AAT BIOQUEST
Description: SMCC is a non-cleavable and membrane permeable crosslinker with a spacer arm of 8.3 Å. It contains an amine-reactive succinimidyl ester (SE) and a sulfhydryl-reactive maleimide group. NHS esters react with primary amines at pH 7 to 9 to form stable amide bonds. Maleimides react with sulfhydryl groups at pH 6.5 to 7.5 to form stable thioether bonds. The SE group of SMCC is reacted with lysine residues on the carrier protein, converting them to reactive maleimides. SMCC is a reagent that is widely used for generating stable maleimide-activated carrier proteins that can spontaneously react with sulfhydryls. Alternatively these relatively stable maleimide-activated intermediates may be lyophilized and stored for later conjugation to a hapten.

Product Image-PIER22322

Sulpho-SMCC (3-Sulpho-N-succinimidyl-4-(N-maleimidomethyl)cyclohexane-1-carboxylate sodium salt), Pierce™

Supplier: Thermo Fisher Scientific
Description: Thermo Scientific Pierce Sulfo-SMCC, No-Weigh Format is a water-soluble, amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a medium-length cyclohexane spacer arm (8.3 angstroms).

Product Image-AATB218

6-NED maleimide fluorescent probe

Catalog Number: (AATB218)
Supplier: AAT BIOQUEST
Description: 5'-Labeled primer pairs include a fluorescently labeled oligo with a choice of dye on the 5' end, along with an unlabeled oligo, in each pair.
UOM: 1 * 1 mg
Image Unavailable-PIERA39268

Sulpho-SMCC (3-Sulpho-N-succinimidyl-4-(N-maleimidomethyl)cyclohexane-1-carboxylate sodium salt), No-Weigh™ Format, Pierce™

Catalog Number: (PIERA39268)
Supplier: Thermo Fisher Scientific
Description: Thermo Scientific Pierce Sulfo-SMCC, No-Weigh Format is a water-soluble, amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a medium-length cyclohexane spacer arm (8.3 angstroms).
UOM: 1 * 20 mg
Product Image-AATB67031

NED Dye qPCR Calibration Solution *10000X*

Catalog Number: (AATB67031)
Supplier: AAT BIOQUEST
Description: NED dye qPCR Calibration Solution supplied as a 10000X concentrate is compatible with a wide variety of qPCR instruments, including the ABI7500 Fast, QuantStudio™, and ViiA™ seven systems.
UOM: 1 * 100 µl

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Product Image-AATB216

6-NED alkyne fluorescent probe

Catalog Number: (AATB216)
Supplier: AAT BIOQUEST
Description: 5'-Labeled primer pairs include a fluorescently labeled oligo with a choice of dye on the 5' end, along with an unlabeled oligo, in each pair.
UOM: 1 * 1 mg
Product Image-USBI038843-FITC

Anti-NDFIP1 Rabbit Polyclonal Antibody (FITC (Fluorescein Isothiocyanate))

Catalog Number: (USBI038843-FITC)
Supplier: US Biological
Description: Anti-NDFIP1 Rabbit Polyclonal Antibody (FITC (Fluorescein Isothiocyanate))
UOM: 1 * 200 µl
Image Unavailable-ICNA0219485794

Urea ≥98%, white powder for molecular biology

Supplier: MP Biomedicals
Description: Applications:
Urea is used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies and in the extraction of the mitochondrial complex. It is commonly used to solubilize and denature proteins for denaturing isoelectric focusing and two-dimensional electrophoresis and in acetic acid-urea PAGE gels. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form. Urea is used in cell or tissue culture media to increase the osmolality. Urea has also been used as fertilizer because of the easy availability of nitrogen; in animal feeds; it is reacted with aldehydes to make resins and plastics; condensed with malonic ester to form barbituric acid; used in the paper industry to soften cellulose.
Biochem/physiol Actions:
Urea has been shown to act as an aldosterone antagonist in the development of peanut agglutinin binding in cultured embryonic renal collecting duct epithelial cells. Urea is the principal end product of nitrogen metabolism in most mammals, formed by the enzymatic reactions of the Kreb's cycle.
Typical Working Concentration:
The use of 2 g/L urea in the culture of Kluyveromyces marxianus to produce a thermostable extracellular lipase has been described. Urea is typically used at a concentration of 8 M for protein denaturation or solubilization. A final concentration of 5 M urea is commonly used in molecular biology for sequencing gels. To prevent carbamylation, do not heat urea containing buffers above 37 °C

Image Unavailable-AATB67010

NED Dye qPCR Calibration Plate *Optimised for ABI7500 Fast 96-Well*

Catalog Number: (AATB67010)
Supplier: AAT BIOQUEST
Description: The NED dye qPCR calibration plate can be used to maintain the 7500 real-time PCR system with fast 96-well block.
UOM: 1 * 1 items

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on +353 1 88 22222
This product is marked as restricted and can only be purchased by approved Shipping Accounts. If you need further assistance, email VWR Regulatory Department at eurega_services@eu.vwr.com
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