You Searched For: 6-NED+NHS+ester

Catalog Number: (AATB3010)

Supplier: AAT BIOQUEST

Description: This biotin has a spacer arm of ~22 Å that reduces steric hindrance when binding several biotinylated molecules to one avidin complex; This reagent penetrates cell membranes because there is no charged group.

UOM: 1 * 25 mg

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Catalog Number: (AATB345)

Supplier: AAT BIOQUEST

Description: 5-CR6G, SE is amine-reactive derivative of 5-CR6G.

UOM: 1 * 5 mg

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Catalog Number: (PIER22339)

Supplier: Thermo Fisher Scientific

Description: Thermo Scientific Pierce SBAP is a short (6.2 angstrom) crosslinker for amine-to-sulfhydryl conjugation via N-hydroxysuccinimide (NHS) ester and bromoacetyl reactive groups.

UOM: 1 * 50 mg

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Supplier: Thermo Fisher Scientific

Description: Cross linkers are used for the identification of near-neighbour protein relationships and ligand-receptor interactions. Homobifunctional, amine-reactive NHS esters or imidates, and heterobifunctional, amine-reactive, photoactivatable phenyl azides are the most commonly used crosslinkers for these applications.

Catalog Number: (RPN5661)

Supplier: Cytiva

Description: CyDye™ Post-labelling reactive dye pack for the generation of highly fluorescent Cy3- and Cy5-labelled probes.

UOM: 1 * 1 KIT

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Catalog Number: (AATB21242)

Supplier: AAT BIOQUEST

Description: Protonex™ Lyso-NIR 770 is a fluorescent derivative prepared from our Protonex™ NIR 770 NHS ester.

UOM: 1 * 1 mg

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Supplier: AAT BIOQUEST

Description: 6-CR6G, SE is amine-reactive derivative of 6-CR6G.

Catalog Number: (PIER26169)

Supplier: Thermo Fisher Scientific

Description: Thermo Scientific Pierce SDAD (NHS-SS-Diazirine) combines proven NHS-ester and diazirine-based photoreaction chemistries with conjugate amine-containing molecules with nearly any other functional group via long-wave UV-light activation. A 13.5Å spacer arm containing a cleavable disulfide bond separates the two photoreactive groups.

UOM: 1 * 50 mg

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Supplier: Cytiva

Description: Mono-reactive dye pack contains derivatised CyDye™ that carries only one reactive group on each dye molecule for targeted and specific labelling of amine groups, for example on antibodies, peptides or oligonucleotides.

Supplier: Cytiva

Description: Amersham™ CyDye™ fluors combine exceptional photostability with bright and intense signals, resulting in high sensitivity and a highly reproducible performance at a biologically suitable pH range of 3 to 10.

Catalog Number: (AATB211)

Supplier: AAT BIOQUEST

Description: 6-TET, SE is a popular amino-reactive fluorescent probe that is widely used in nucleic acid sequencing and related research.

UOM: 1 * 5 mg

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Catalog Number: (PIER22298)

Supplier: Thermo Fisher Scientific

Description: Thermo Scientific Pierce BMPS is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a short spacer arm (5.9 Å).

UOM: 1 * 50 mg

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Supplier: Thermo Fisher Scientific

Description: Thermo Scientific Pierce DSP (Lomant's Reagent) is a water-insoluble, homo-bifunctional N-hydroxysuccimide ester (NHS ester) crosslinker that is thiol-cleavable, primary amine-reactive, and useful for many applications. DSP contains an amine-reactive NHS ester at each end of an 8-carbon spacer arm. NHS esters react with primary amines at pH 7–9 to form stable amide bonds and releasing N-hydroxy-succinimide. Proteins, including antibodies, generally have several primary amines in the side chain of lysine (K) residues and the N-terminus of each polypeptide that are available as targets for NHS ester crosslinking reagents. DSP is non-sulfonated and insoluble in water, so it must first be dissolved in an organic solvent and then added to the aqueous reaction mixture. Because DSP does not possess a charged group, it is lipophilic and membrane-permeable and so useful for intracellular and intramembrane conjugation. A sulfonated analog of DSP (DTTSP) is water soluble. DSS, the non-cleavable analog of the DSP crosslinker is also available for applications that require a stable spacer arm that cannot be cleaved in the presence of reducing agents.

Catalog Number: (PIER22360)

Supplier: Thermo Fisher Scientific

Description: Thermo Scientific Pierce SMCC is a hetero-bifunctional crosslinker that contain N-hydroxysuccinimide (NHS) ester and maleimide groups that allow covalent conjugation of amine- and sulfhydryl-containing molecules. NHS esters react with primary amines at pH 7–9 to form amide bonds, while maleimides react with sulfhydryl groups at pH 6.5–7.5 to form stable thioether bonds. In aqueous solutions, NHS ester hydrolytic degradation is a competing reaction whose rate increases with pH. The maleimide group is more stable than the NHS-ester group, but will slowly hydrolyze and lose its reaction specificity for sulfhydryls at pH values > 7.5. For these reasons, conjugations with these crosslinkers are usually performed at pH 7.2–7.5, with the NHS ester (amine-targeted) reacted before or simultaneous with the maleimide (sulfhydryl-targeted) reaction.

UOM: 1 * 50 mg

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Catalog Number: (PIER22311)

Supplier: Thermo Fisher Scientific

Description: Thermo Scientific Pierce MBS is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a short aromatic spacer arm (7.3 angstroms).

UOM: 1 * 50 mg

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Catalog Number: (PIER22416)

Supplier: Thermo Fisher Scientific

Description: Thermo Scientific Pierce SMPB is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a medium-length aromatic spacer arm (11.6 angstroms).

UOM: 1 * 50 mg

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