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Product Image-PIER21336

Amine reactive biotinylation reagents, EZ-Link™

Supplier: Thermo Fisher Scientific
Description: The amine group is the most common target for modifying protein molecules because it is abundant in the majority of proteins either due to the presence of lysine bearing amino side chain functionality or the N-terminal-amine. Amine-reactive biotinylation reagents can be divided into two groups based on water solubility: NHS-esters and sulfo-NHS-esters.

Product Image-PIER22360

SMCC (N-Succinimidyl 4-(N-Maleimidomethyl)cyclohexanecarboxylate), Pierce™

Catalog Number: (PIER22360)
Supplier: Thermo Fisher Scientific
Description: Thermo Scientific Pierce SMCC is a hetero-bifunctional crosslinker that contain N-hydroxysuccinimide (NHS) ester and maleimide groups that allow covalent conjugation of amine- and sulfhydryl-containing molecules. NHS esters react with primary amines at pH 7–9 to form amide bonds, while maleimides react with sulfhydryl groups at pH 6.5–7.5 to form stable thioether bonds. In aqueous solutions, NHS ester hydrolytic degradation is a competing reaction whose rate increases with pH. The maleimide group is more stable than the NHS-ester group, but will slowly hydrolyze and lose its reaction specificity for sulfhydryls at pH values > 7.5. For these reasons, conjugations with these crosslinkers are usually performed at pH 7.2–7.5, with the NHS ester (amine-targeted) reacted before or simultaneous with the maleimide (sulfhydryl-targeted) reaction.
UOM: 1 * 50 mg

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Image Unavailable-PIERPG82078

Sulpho-NHS-SS-Biotin, Premium Grade, Pierce™

Supplier: Thermo Fisher Scientific
Description: Thermo Scientific™ Pierce™ Premium Grade Sulfo-NHS-SS-Biotin is our highest quality formulation of this amine-reactive, cleavable biotinylation reagent, specially characterized for applications where product integrity and risk minimization are paramount. Sulfo-NHS-SS-biotin is used for cleavable attachment of biotin to protein and cell surface amines.

Product Image-PIER22341

Pegylation reagents, Methyl-PEG-NHS-Esters, Pierce™

Supplier: Thermo Fisher Scientific
Description: Pierce™ MS(PEG)<sub>n</sub> reagents are methyl-terminated, polyethylene glycol compounds (n equals 4 to 24 PEG units) and TMS(PEG)<sub>12</sub> is a three-branched, polyethylene glycol compound (3× 12 PEG units). Both are activated as NHS esters for covalent pegylation of primary amines on proteins (eg. lysines) or assay surfaces.

Product Image-PIER24500

N-Hydroxysuccinimide, Pierce™

Catalog Number: (PIER24500)
Supplier: Thermo Fisher Scientific
Description: Thermo Scientific Pierce NHS is a chemical modification reagent for converting carboxyl groups to amine-reactive NHS esters for bioconjugation, crosslinking, labeling and immobilization methods.
UOM: 1 * 25 g
Image Unavailable-PIERA35394

SMCC (N-Succinimidyl 4-(N-Maleimidomethyl)cyclohexanecarboxylate), No-Weigh™ Format, Pierce™

Catalog Number: (PIERA35394)
Supplier: Thermo Fisher Scientific
Description: Thermo Scientific Pierce SMCC is a hetero-bifunctional crosslinker that contain N-hydroxysuccinimide (NHS) ester and maleimide groups that allow covalent conjugation of amine- and sulfhydryl-containing molecules. NHS esters react with primary amines at pH 7–9 to form amide bonds, while maleimides react with sulfhydryl groups at pH 6.5–7.5 to form stable thioether bonds. In aqueous solutions, NHS ester hydrolytic degradation is a competing reaction whose rate increases with pH. The maleimide group is more stable than the NHS-ester group, but will slowly hydrolyze and lose its reaction specificity for sulfhydryls at pH values > 7.5. For these reasons, conjugations with these crosslinkers are usually performed at pH 7.2–7.5, with the NHS ester (amine-targeted) reacted before or simultaneous with the maleimide (sulfhydryl-targeted) reaction.
UOM: 1 * 10 mg
Product Image-PIER22324

Sulpho-GMBS (N-Maleimidobutyryloxysulphosuccinimide ester), Pierce™

Catalog Number: (PIER22324)
Supplier: Thermo Fisher Scientific
Description: Thermo Scientific Pierce Sulfo-GMBS is a water-soluble amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a short spacer arm (7.3 angstroms).
UOM: 1 * 50 mg
Product Image-PIER26173

Sulpho-SDA (Sulpho-NHS-Diazirine) (Sulphosuccinimidyl 4,4′-azipentanoate), Pierce™

Catalog Number: (PIER26173)
Supplier: Thermo Fisher Scientific
Description: Thermo Scientific Pierce Sulfo-SDA (Sulfo-NHS-Diazirine) combines proven NHS-ester and diazirine-based photoreaction chemistries with conjugate amine-containing molecules with nearly any other functional group via long-wave UV-light activation. A 3.9Å spacer arm separates the two photoreactive groups.
UOM: 1 * 50 mg
Product Image-AATB345

5-CR6G, SE single isomer ≥90%

Catalog Number: (AATB345)
Supplier: AAT BIOQUEST
Description: 5-CR6G, SE is amine-reactive derivative of 5-CR6G.
UOM: 1 * 5 mg
Product Image-PIER22339

SBAP (Succinimidyl 3-(bromoacetamido)propionate), Pierce™

Catalog Number: (PIER22339)
Supplier: Thermo Fisher Scientific
Description: Thermo Scientific Pierce SBAP is a short (6.2 angstrom) crosslinker for amine-to-sulfhydryl conjugation via N-hydroxysuccinimide (NHS) ester and bromoacetyl reactive groups.
UOM: 1 * 50 mg
Product Image-PIER21945

Amine reactive biotinylation kits, EZ-Link™

Supplier: Thermo Fisher Scientific
Description: The amine group is the most common target for modifying protein molecules because it is abundant in the majority of proteins either due to the presence of lysine bearing amino side chain functionality or the N-terminal-amine. Amine-reactive biotinylation reagents can be divided into two groups based on water solubility: NHS-esters and sulfo-NHS-esters.

Product Image-AATB570

3-Carboxy-6,8-difluoro-7-hydroxycoumarin ≥90% [equivalent to Pacific Blue succinimidyl ester]

Catalog Number: (AATB570)
Supplier: AAT BIOQUEST
Description: PacBlue succinimidyl ester is the same molecule to Pacific Blue™ succinimidyl ester (Pacific Blue™ is the trademark of ThermoFisher).
UOM: 1 * 5 mg
Image Unavailable-AATB21242

Protonex™ Lyso-NIR 770

Catalog Number: (AATB21242)
Supplier: AAT BIOQUEST
Description: Protonex™ Lyso-NIR 770 is a fluorescent derivative prepared from our Protonex™ NIR 770 NHS ester.
UOM: 1 * 1 mg

New Product


Product Image-PIER53032

Speciality dyes, DyLight™

Supplier: Thermo Fisher Scientific
Description: The DyLight™ Specialty Dyes are fluorescent dyes with varying spectral characteristics, degrees of sulfonation, charges and hydrophobicities. These dyes are offered in their amine-reactive forms and are mainly derivatized with N-hydroxysuccinimide (NHS) esters, the simplest and most commonly used reactive group for labeling proteins. The large selection of near infrared (NIR) and infrared (IR) dyes may be used for<i> in vivo</i> and near-IR fluorescence (NIRF) applications.

Product Image-PA63106

CyDye™ Mono-Reactive Maleimide Fluorescent Dyes, Cytiva

Supplier: Cytiva
Description: CyDye™ Mono-Reactive Maleimide Fluorescent Dyes are intensely fluorescent and highly water soluble, providing significant advantages over other existing fluorophores.

Product Image-AATB119

5-FAM-X, SE ≥95%

Catalog Number: (AATB119)
Supplier: AAT BIOQUEST
Description: 5-FAM-X, SE contains the aminohexanoyl spacer (known as 'X') between the FAM fluorophore and the succinimidyl ester.
UOM: 1 * 5 mg
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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on +353 1 88 22222
This product is marked as restricted and can only be purchased by approved Shipping Accounts. If you need further assistance, email VWR Regulatory Department at eurega_services@eu.vwr.com
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