You Searched For: 6-Chloro-3-indolyl+\u03B1-D-mannopyranoside

Supplier: Thermo Fisher Scientific

Description: 5-Bromo-4-chloro-3-indolyl phosphate disodium salt (BCIP-Na) ≥98%

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Catalog Number: (MOLEM46642783)

Supplier: Molekula

Description: 5-Bromo-4-chloro-3-indolyl phosphate disodium salt (BCIP-Na)

UOM: 1 * 1 g

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Catalog Number: (OZBIXG31000)

Supplier: OZ BIOSCIENCES

Description: X-Gal substrate is metabolised by the b-galactosidase enzyme. The quality and purity of the substrate is essential to obtain high-quality and reliable results.

UOM: 1 * 1 g

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Supplier: MP Biomedicals

Description: 5-Bromo-4-chloro-3-indolyl b-D-galactopyranoside, commonly known as X-Gal, is a histochemical substrate for b-galactosidase. X-Gal is cleaved by b-galactosidase to yield an insoluble blue precipitate.

Supplier: MP Biomedicals

Description: Storage: Store at -20 °C. Store Desiccated. Store Under Nitrogen. Protect from light.
5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside, commonly known as X−Gal, is a histochemical substrate for β−galactosidase.
5-Bromo-4-Chloro-3-Indolyl-β-D-Galactopyranoside is used as indigogenic substrate for β-galactosidase, for detection of β-galactosidase-positive clones, and the identification of lac and bacterial colonies or phage plaques. It is the substrate of choice for blue-white selection of recombinant bacterial colonies with the lac+ genotype. X−Gal is cleaved by β−galactosidase to yield an insoluble blue precipitate. X−Gal is particularly useful in molecular biology applications to detect the activity of β−galactosidase which is frequently used as a reporter gene. In cloning, X−Gal is used to detect insertion of foreign DNA into the lacZ region of plasmid DNA using α-complementation which is based on vectors such as the pUC and the M13mp series that carry a fragment of the β-galactosidase gene encoding an α-fragment of β-galactosidase.

Catalog Number: (GENORC-214)

Supplier: G-Biosciences

Description: X-Gluc (5-bromo-4-chloro-3-indolyl-β-D-glucuronic acid) is a substrate for ß-glucuronidase (GUS), which is encoded by <italic>gusA</italic>, a widely used reporter gene. The glucuronidase cleaves X-Gluc to produce colourless glucuronic acid and an intense blue precipitate of chloro-bromoindigo. X-Gluc is used for the histochemical detection of GUS expression in plant cells and tissues, detection of infections caused by <italic>E.coli</italic> and detection of bacterial contamination in food and water samples.

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Supplier: Apollo Scientific

Description: Methyl 3-amino-3-deoxy-α-D-mannopyranoside hydrochloride ≥98%

Supplier: Apollo Scientific

Description: 2-Azidoethyl 2,3,4,6-tetra-O-acetyl-α-D-mannopyranoside ≥98%

Catalog Number: (OZBIGX10003)

Supplier: OZ BIOSCIENCES

Description: X-Gal staining kit allows to visualise ß-gal expression through hydrolysis of the X-Gal (5-bromo-4-chloro-3-indoyl-beta-D-Galactopyranoside) substrate yielding blue precipitates.

UOM: 1 * 1 KIT

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Supplier: G-Biosciences

Description: X-gal (5-bromo-4-chloro-3-indolyl-β-D- galactopyranoside) is used to indicate whether bacteria express the ß-galactosidase enzyme, which is encoded by the lacZ gene. X-gal is cleaved by ß-galactosidase yielding galactose and 5-bromo-4-chloro-3-hydroxyindole, which is oxidised into 5,5'-dibromo-4,4'-dichloro-indigo, an insoluble blue product. Thus, if X-gal and an inducer of ß-galactosidase (usually IPTG) is contained within an agar medium on a culture plate, colonies which have a functional lacZ gene can easily be distinguished by their blue colour.

Catalog Number: (10026.)

Supplier: Biotium

Description: X-β-D-cellobioside is a chromogenic substrate for β-cellobiosidase.

UOM: 1 * 25 mg

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Catalog Number: (462711-100)

Supplier: Merck Millipore (Calbiochem‎)

Description: Methyl-α-D-mannopyranoside is a substrate for α1,2-mannosyltransferase.

UOM: 1 * 100 g

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Supplier: MP Biomedicals

Description: p-Nitroblue Tetrazolium Chloride is an NADPH-diaphorase substrate that competitively inhibits NOS (nitric oxide synthase) (IC50 = 3-4 μM). In combination with 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and an appropriate reaction buffer, Nitroblue tetrazolium forms a substrate for the colorimetric detection of alkaline phosphatase conjugates in a variety of molecular and cell biology techniques.

p-nitro blue tetrazolium is used for the detection of alkaline phosphatase on western blots. It has also been used as a redox indicator for other enzymatic reactions including dehydrogenases, threonine deaminase, glucose-6-phosphate dehydrogenase, phosphofructokinase on polyacrylamide gels, oxidases on polyacrylamide gels, and pentose shunt dehydrogenases. Redox and halfwave potentials have been determined for p - nitro blue tetrazolium. It has also been used as a colorimetric indicator of bacterial infection in blood samples.

Catalog Number: (I7513-5MG)

Supplier: SIGMA ALDRICH MICROSCOPY

Description: 3-Indolyl-ß-D-galactopyranoside, Sigma-Aldrich®

UOM: 1 * 5 mg

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Supplier: Apollo Scientific

Description: 3-Indolyl butyrate

Supplier: MP Biomedicals

Description: Used to reduce Con A binding to lymphocyte surface in culture.