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Catalog Number: (OZBIGX10003)
Supplier: OZ BIOSCIENCES
Description: X-Gal staining kit allows to visualise ß-gal expression through hydrolysis of the X-Gal (5-bromo-4-chloro-3-indoyl-beta-D-Galactopyranoside) substrate yielding blue precipitates.
UOM: 1 * 1 KIT


Catalog Number: (203788-1)
Supplier: Merck Millipore (Oncogene)
Description: 5-Bromo-4-chloro-3-indolyl phosphate disodium salt (BCIP-Na), Sigma-Aldrich®
UOM: 1 * 1 g


Catalog Number: (MOLEM30473975)
Supplier: Molekula
Description: X-Gal (5-bromo-4-chloro-3-indolyl-ß-D-galactopyranoside)
UOM: 1 * 100 mg

Market Source Item This is a MarketSource item. Additional charges may apply

Catalog Number: (MOLE11322533-5G)
Supplier: Molekula
Description: X-Gal (5-bromo-4-chloro-3-indolyl-ß-D-galactopyranoside)
UOM: 1 * 5 g

Market Source Item This is a MarketSource item. Additional charges may apply

Catalog Number: (RC-113)
Supplier: G-Biosciences
Description: X-Gal (5-bromo-4-chloro-3-indolyl-ß-D-galactopyranoside)
UOM: 1 * 100 mg


Catalog Number: (MOLEM41538013)
Supplier: Molekula
Description: 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt (BCIP (p-toluidine salt), X-Phosphate)
UOM: 1 * 1 g

Market Source Item This is a MarketSource item. Additional charges may apply

Supplier: G-Biosciences
Description: X-gal (5-bromo-4-chloro-3-indolyl-β-D- galactopyranoside) is used to indicate whether bacteria express the ß-galactosidase enzyme, which is encoded by the lacZ gene. X-gal is cleaved by ß-galactosidase yielding galactose and 5-bromo-4-chloro-3-hydroxyindole, which is oxidised into 5,5'-dibromo-4,4'-dichloro-indigo, an insoluble blue product. Thus, if X-gal and an inducer of ß-galactosidase (usually IPTG) is contained within an agar medium on a culture plate, colonies which have a functional lacZ gene can easily be distinguished by their blue colour.

Catalog Number: (10016.)
Supplier: Biotium
Description: X-GlcA ((5-bromo-4-chloro-3-indolyl-ß-D-glucuronide) cyclohexylammonium salt)
UOM: 1 * 25 mg


Catalog Number: (OZBIXG31000)
Supplier: OZ BIOSCIENCES
Description: X-Gal substrate is metabolised by the b-galactosidase enzyme. The quality and purity of the substrate is essential to obtain high-quality and reliable results.
UOM: 1 * 1 g


Supplier: Thermo Fisher Scientific
Description: 5-Bromo-4-chloro-3-indolyl phosphate disodium salt (BCIP-Na) ≥98%
Supplier: MP Biomedicals
Description: Storage: Store at -20 °C. Store Desiccated. Store Under Nitrogen. Protect from light.
5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside, commonly known as X−Gal, is a histochemical substrate for β−galactosidase.
5-Bromo-4-Chloro-3-Indolyl-β-D-Galactopyranoside is used as indigogenic substrate for β-galactosidase, for detection of β-galactosidase-positive clones, and the identification of lac and bacterial colonies or phage plaques. It is the substrate of choice for blue-white selection of recombinant bacterial colonies with the lac+ genotype. X−Gal is cleaved by β−galactosidase to yield an insoluble blue precipitate. X−Gal is particularly useful in molecular biology applications to detect the activity of β−galactosidase which is frequently used as a reporter gene. In cloning, X−Gal is used to detect insertion of foreign DNA into the lacZ region of plasmid DNA using α-complementation which is based on vectors such as the pUC and the M13mp series that carry a fragment of the β-galactosidase gene encoding an α-fragment of β-galactosidase.

Catalog Number: (GENORC-214)
Supplier: G-Biosciences
Description: X-Gluc (5-bromo-4-chloro-3-indolyl-β-D-glucuronic acid) is a substrate for ß-glucuronidase (GUS), which is encoded by <italic>gusA</italic>, a widely used reporter gene. The glucuronidase cleaves X-Gluc to produce colourless glucuronic acid and an intense blue precipitate of chloro-bromoindigo. X-Gluc is used for the histochemical detection of GUS expression in plant cells and tissues, detection of infections caused by <italic>E.coli</italic> and detection of bacterial contamination in food and water samples.
UOM:


Catalog Number: (10026.)
Supplier: Biotium
Description: X-β-D-cellobioside is a chromogenic substrate for β-cellobiosidase.
UOM: 1 * 25 mg


Supplier: Apollo Scientific
Description: 6-Chloro-3-indolyl β-D-glucopyranoside

Supplier: Apollo Scientific
Description: 6-Chloro-3-indolyl α-D-mannopyranoside

Supplier: Apollo Scientific
Description: 6-Chloro-3-indolyl α-glucopyranoside

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on +353 1 88 22222
This product is marked as restricted and can only be purchased by approved Shipping Accounts. If you need further assistance, email VWR Regulatory Department at eurega_services@eu.vwr.com
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The original product is no longer available. The replacement shown is available.
Product(s) marked with this symbol are discontinued - sold till end of stock. Alternatives may be available by searching with the VWR Catalog Number listed above. If you need further assistance, please call VWR Customer Service on +353 1 8822222.
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