You Searched For: 5(6)-Carboxyfluorescein+N-hydroxysuccinimide+ester+(5\/6-FAM+SE)

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6-Carboxy-4,7-dichlororhodamine 110 (6-dR110) ≥95%

Catalog Number: (AATB316)

Supplier: AAT BIOQUEST

Description: Compared to fluorescein labeling reagents such as FITC and FAM, 6-dR110 [6-carboxy-4,7-dichlororhodamine 110] reagents give more photostable and pH-independent bioconjugates that have the absorption maximum around the preferred 488 nm excitation wavelength.

UOM: 1 * 25 mg

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Real Time PCR Detection Test Kits, Supreme HLVd (Hop Latent Viroid)

Supplier: BIOPREMIER

Description: SUPREME RT-PCR detection kit HLVd is designed for the detection of Hop Latent Viroid (HLVd) in a single PCR reaction. The kit is suitable for use in real time instruments with FAM and Cy5 detection channels.

Affinity coupling media, NHS-Activated Sepharose™ 4 Fast Flow

Catalog Number: (17-0906-01)

Supplier: Cytiva

Description: NHS-activated Sepharose™ 4 Fast Flow is composed of cross-linked 4% agarose beads, NHS activated for coupling amino-containing proteins and peptides in process-scale applications. NHS (N-hydroxysuccinimide) coupling forms a chemically stable amide bond with ligands containing primary amino groups. The resin provides a spacer arm to the coupled ligand and suitable for immobilising smaller protein and peptide ligands.

UOM: 1 * 25 mL

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EDC-HCl (N-(3-Dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride), Pierce™

Supplier: Thermo Fisher Scientific

Description: Thermo Scientific Pierce EDC is a carboxyl- and amine-reactive zero-length crosslinker. EDC reacts with a carboxyl group first and forms an amine-reactive O-acylisourea intermediate that quickly reacts with an amino group to form an amide bond with release of an isourea by-product. The intermediate is unstable in aqueous solutions and so two-step conjugation procedures rely on N-hydroxysuccinimide (NHS) for stabilization. Failure to react with an amine will result in hydrolysis of the intermediate, regeneration of the carboxyl, and release of an N-substituted urea. A side reaction is the formation of an N-acylurea, which is usually restricted to carboxyls located in hydrophobic regions of proteins.

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