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Image Unavailable-BOSSBS-0987R-CY5

Anti-BCHE Rabbit Polyclonal Antibody (Cy5®)

Description: Esterase with broad substrate specificity. Contributes to the inactivation of the neurotransmitter acetylcholine. Can degrade neurotoxic organophosphate esters.
Catalog Number: BOSSBS-0987R-CY5
UOM: 1 * 100 µl
Supplier: Bioss
Image Unavailable-BOSSBS-1135R-CY5

Anti-SRC Rabbit Polyclonal Antibody (Cy5®)

Description: c-Src tyrosine kinase plays a critical role in signal transduction downstream of growth factor receptors, integrins and G protein-coupled receptors. We used stable isotope labeling with amino acids in cell culture (SILAC) approach to identify additional substrates of c-Src tyrosine kinase in human embryonic kidney 293T cells. We have identified 10 known substrates and interactors of c-Src and Src family kinases along with 26 novel substrates. We have experimentally validated 4 of the novel proteins (NICE-4, RNA binding motif 10, FUSE-binding protein 1 and TRK-fused gene) as direct substrates of c-Src using in vitro kinase assays and cotransfection experiments. Significantly, using a c-Src specific inhibitor, we were also able to implicate 3 novel substrates (RNA binding motif 10, EWS1 and Bcl-2 associated transcription factor) in PDGF signaling. Finally, to identify the exact tyrosine residues that are phosphorylated by c-Src on the novel c-Src substrates, we designed custom peptide microarrays containing all possible tyrosine-containing peptides (312 unique peptides) and their mutant counterparts containing a Tyr -->Phe substitution from 14 of the identified substrates. Using this platform, we identified 34 peptides that are phosphorylated by c-Src. We have demonstrated that SILAC-based quantitative proteomics approach is suitable for identification of substrates of nonreceptor tyrosine kinases and can be coupled with peptide microarrays for high-throughput identification of substrate phosphopeptides.
Catalog Number: BOSSBS-1135R-CY5
UOM: 1 * 100 µl
Supplier: Bioss
Image Unavailable-BOSSBS-1135R-HRP

Anti-SRC Rabbit Polyclonal Antibody (HRP (Horseradish Peroxidase))

Description: c-Src tyrosine kinase plays a critical role in signal transduction downstream of growth factor receptors, integrins and G protein-coupled receptors. We used stable isotope labeling with amino acids in cell culture (SILAC) approach to identify additional substrates of c-Src tyrosine kinase in human embryonic kidney 293T cells. We have identified 10 known substrates and interactors of c-Src and Src family kinases along with 26 novel substrates. We have experimentally validated 4 of the novel proteins (NICE-4, RNA binding motif 10, FUSE-binding protein 1 and TRK-fused gene) as direct substrates of c-Src using in vitro kinase assays and cotransfection experiments. Significantly, using a c-Src specific inhibitor, we were also able to implicate 3 novel substrates (RNA binding motif 10, EWS1 and Bcl-2 associated transcription factor) in PDGF signaling. Finally, to identify the exact tyrosine residues that are phosphorylated by c-Src on the novel c-Src substrates, we designed custom peptide microarrays containing all possible tyrosine-containing peptides (312 unique peptides) and their mutant counterparts containing a Tyr -->Phe substitution from 14 of the identified substrates. Using this platform, we identified 34 peptides that are phosphorylated by c-Src. We have demonstrated that SILAC-based quantitative proteomics approach is suitable for identification of substrates of nonreceptor tyrosine kinases and can be coupled with peptide microarrays for high-throughput identification of substrate phosphopeptides.
Catalog Number: BOSSBS-1135R-HRP
UOM: 1 * 100 µl
Supplier: Bioss
Image Unavailable-BOSSBS-1135R-A555

Anti-SRC Rabbit Polyclonal Antibody (Alexa Fluor® 555)

Description: c-Src tyrosine kinase plays a critical role in signal transduction downstream of growth factor receptors, integrins and G protein-coupled receptors. We used stable isotope labeling with amino acids in cell culture (SILAC) approach to identify additional substrates of c-Src tyrosine kinase in human embryonic kidney 293T cells. We have identified 10 known substrates and interactors of c-Src and Src family kinases along with 26 novel substrates. We have experimentally validated 4 of the novel proteins (NICE-4, RNA binding motif 10, FUSE-binding protein 1 and TRK-fused gene) as direct substrates of c-Src using in vitro kinase assays and cotransfection experiments. Significantly, using a c-Src specific inhibitor, we were also able to implicate 3 novel substrates (RNA binding motif 10, EWS1 and Bcl-2 associated transcription factor) in PDGF signaling. Finally, to identify the exact tyrosine residues that are phosphorylated by c-Src on the novel c-Src substrates, we designed custom peptide microarrays containing all possible tyrosine-containing peptides (312 unique peptides) and their mutant counterparts containing a Tyr -->Phe substitution from 14 of the identified substrates. Using this platform, we identified 34 peptides that are phosphorylated by c-Src. We have demonstrated that SILAC-based quantitative proteomics approach is suitable for identification of substrates of nonreceptor tyrosine kinases and can be coupled with peptide microarrays for high-throughput identification of substrate phosphopeptides.
Catalog Number: BOSSBS-1135R-A555
UOM: 1 * 100 µl
Supplier: Bioss
Image Unavailable-BOSSBS-0987R-A488

Anti-BCHE Rabbit Polyclonal Antibody (Alexa Fluor® 488)

Description: Esterase with broad substrate specificity. Contributes to the inactivation of the neurotransmitter acetylcholine. Can degrade neurotoxic organophosphate esters.
Catalog Number: BOSSBS-0987R-A488
UOM: 1 * 100 µl
Supplier: Bioss
Image Unavailable-BOSSBS-0987R-A350

Anti-BCHE Rabbit Polyclonal Antibody (Alexa Fluor® 350)

Description: Esterase with broad substrate specificity. Contributes to the inactivation of the neurotransmitter acetylcholine. Can degrade neurotoxic organophosphate esters.
Catalog Number: BOSSBS-0987R-A350
UOM: 1 * 100 µl
Supplier: Bioss
Image Unavailable-ENZOALX850216KI01

Caspase-3 fluorometric assay kit

Description: Based on the detection of AFC (7-amino-4-trifluoromethyl coumarin) after cleavage from the labelled substrate DEVD-AFC.
Catalog Number: ENZOALX850216KI01
UOM: 1 * 25 Tests
Supplier: ENZO LIFE SCIENCES
Image Unavailable-BOSSBS-1135R-CY3

Anti-SRC Rabbit Polyclonal Antibody (Cy3®)

Description: c-Src tyrosine kinase plays a critical role in signal transduction downstream of growth factor receptors, integrins and G protein-coupled receptors. We used stable isotope labeling with amino acids in cell culture (SILAC) approach to identify additional substrates of c-Src tyrosine kinase in human embryonic kidney 293T cells. We have identified 10 known substrates and interactors of c-Src and Src family kinases along with 26 novel substrates. We have experimentally validated 4 of the novel proteins (NICE-4, RNA binding motif 10, FUSE-binding protein 1 and TRK-fused gene) as direct substrates of c-Src using in vitro kinase assays and cotransfection experiments. Significantly, using a c-Src specific inhibitor, we were also able to implicate 3 novel substrates (RNA binding motif 10, EWS1 and Bcl-2 associated transcription factor) in PDGF signaling. Finally, to identify the exact tyrosine residues that are phosphorylated by c-Src on the novel c-Src substrates, we designed custom peptide microarrays containing all possible tyrosine-containing peptides (312 unique peptides) and their mutant counterparts containing a Tyr -->Phe substitution from 14 of the identified substrates. Using this platform, we identified 34 peptides that are phosphorylated by c-Src. We have demonstrated that SILAC-based quantitative proteomics approach is suitable for identification of substrates of nonreceptor tyrosine kinases and can be coupled with peptide microarrays for high-throughput identification of substrate phosphopeptides.
Catalog Number: BOSSBS-1135R-CY3
UOM: 1 * 100 µl
Supplier: Bioss
Image Unavailable-ENZOALX850214KI01

Caspase-2 fluorometric assay kit

Description: Based on the detection of AFC (7-amino-4-trifluoromethyl coumarin) after cleavage from the labelled substrate VDVAD-AFC.
Catalog Number: ENZOALX850214KI01
UOM: 1 * 25 Tests
Supplier: ENZO LIFE SCIENCES
Image Unavailable-BOSSBS-1135R-A680

Anti-SRC Rabbit Polyclonal Antibody (Alexa Fluor® 680)

Description: c-Src tyrosine kinase plays a critical role in signal transduction downstream of growth factor receptors, integrins and G protein-coupled receptors. We used stable isotope labelling with amino acids in cell culture (SILAC) approach to identify additional substrates of c-Src tyrosine kinase in human embryonic kidney 293T cells. We have identified 10 known substrates and interactors of c-Src and Src family kinases along with 26 novel substrates. We have experimentally validated 4 of the novel proteins (NICE-4, RNA binding motif 10, FUSE-binding protein 1 and TRK-fused gene) as direct substrates of c-Src using in vitro kinase assays and cotransfection experiments. Significantly, using a c-Src specific inhibitor, we were also able to implicate 3 novel substrates (RNA binding motif 10, EWS1 and Bcl-2 associated transcription factor) in PDGF signaling. Finally, to identify the exact tyrosine residues that are phosphorylated by c-Src on the novel c-Src substrates, we designed custom peptide microarrays containing all possible tyrosine-containing peptides (312 unique peptides) and their mutant counterparts containing a Tyr --> Phe substitution from 14 of the identified substrates. Using this platform, we identified 34 peptides that are phosphorylated by c-Src. We have demonstrated that SILAC-based quantitative proteomics approach is suitable for identification of substrates of nonreceptor tyrosine kinases and can be coupled with peptide microarrays for high-throughput identification of substrate phosphopeptides.
Catalog Number: BOSSBS-1135R-A680
UOM: 1 * 100 µl
Supplier: Bioss
Image Unavailable-BOSSBS-0987R-CY3

Anti-BCHE Rabbit Polyclonal Antibody (Cy3®)

Description: Esterase with broad substrate specificity. Contributes to the inactivation of the neurotransmitter acetylcholine. Can degrade neurotoxic organophosphate esters.
Catalog Number: BOSSBS-0987R-CY3
UOM: 1 * 100 µl
Supplier: Bioss
Image Unavailable-BOSSBS-8474R-CY5

Anti-FBXL16 Rabbit Polyclonal Antibody (Cy5®)

Description: Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex.
Catalog Number: BOSSBS-8474R-CY5
UOM: 1 * 100 µl
Supplier: Bioss
Image Unavailable-BOSSBS-8474R-CY7

Anti-FBXL16 Rabbit Polyclonal Antibody (Cy7®)

Description: Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex.
Catalog Number: BOSSBS-8474R-CY7
UOM: 1 * 100 µl
Supplier: Bioss
Product Image-AATB13201

D-VLK-AMC [D-Val-Leu-Lys-AMC]

Description: D-VLK-AMC is a sensitive, highly specific fluorescent substrate for plasmin.
Catalog Number: AATB13201
UOM: 1 * 5 mg
Supplier: AAT BIOQUEST
Image Unavailable-BOSSBS-0987R-A647

Anti-BCHE Rabbit Polyclonal Antibody (Alexa Fluor® 647)

Description: Esterase with broad substrate specificity. Contributes to the inactivation of the neurotransmitter acetylcholine. Can degrade neurotoxic organophosphate esters.
Catalog Number: BOSSBS-0987R-A647
UOM: 1 * 100 µl
Supplier: Bioss
Image Unavailable-BOSSBS-8474R-A555

Anti-FBXL16 Rabbit Polyclonal Antibody (Alexa Fluor® 555)

Description: Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex.
Catalog Number: BOSSBS-8474R-A555
UOM: 1 * 100 µl
Supplier: Bioss
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