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You Searched For: Electrophoresis Buffers

The electrophoresis buffers maintain consistent pH levels during DNA, RNA, and other protein separation. Providing ions that transfer the applied current, the low charge solutions facilitate different molecular sizes to travel at various rates for analyses. Offering additional protection to proteins, the preparative substances prevent degradation for guaranteed sample viability and accurate testing. Available in various molecular weights, the best electrophoresis buffer can be selected based on the specific macromolecules being handled.

The electrophoresis buffers maintain consistent pH levels during DNA, RNA, and other protein separation. Providing ions that transfer the applied current, the low charge solutions facilitate different molecular sizes to travel at various rates for analyses. Offering additional protection to proteins, the preparative substances prevent degradation for guaranteed sample viability and accurate testing. Available in various molecular weights, the best electrophoresis buffer can be selected based on the specific macromolecules being handled.


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Product Image-ROCKMB-070

Blocking buffers

Catalog Number: (ROCKMB-070)
Supplier: Rockland Immunochemicals
Description: A blocking buffer specifically designed for western blotting using fluorochrome conjugated antibodies. Blocking buffer was prepared using ultra pure reagents dissolved in pharmaceutical grade water (WFI) and consists of a proprietary protein formulation in tris buffered saline at pH 7,6 with thimerisol added as an antimicrobial agent.
UOM: 1 * 500 mL
Product Image-786-027

Phosphate buffered saline (PBS), 10X

Catalog Number: (786-027)
Supplier: G-Biosciences
Description: PBS 10X solution is used as a wash buffers for Western blotting, ELISA and other applications.
UOM: 1 * 500 mL

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Image Unavailable-A0972.1000

Tris-Borate-EDTA (TBE) buffer, 10X

Supplier: PanReac AppliChem
Description: 10X concentrated solution of 0,89 M Tris, 0,89 M boric acid and 0,02 M EDTA.Na₂, pH 8,3 ±0,2.

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Product Image-786-471

Hybridisation buffers

Supplier: G-Biosciences
Description: The hybridisation buffers like Church & Gilbert’s, Denhardt solution, hybridisation denaturing solution, hybridisation neutralising solution, SSC, SSPE, TMAC Hyb solution and TNT Buffer are used in wide range of applications. Church & Gilbert’s Hybridization Buffer is prepared from molecular biology grade SDS (sodium dodecyl sulfate), monosodium phosphate (NaHPO₄), EDTA (ethylenediaminetetracetic acid) and bovine serum albumin (BSA) Fraction V using DNase, RNase and Protease-free water. It consists of 1mM EDTA, 1% BSA, 0,5M sodium phosphate monobasic, and 7% SDS (at a pH of 7,2).

Product Image-ROCKMB-020

Buffer, TAE solution 50X concentrate (TRIS-acetate-EDTA buffer)

Catalog Number: (ROCKMB-020)
Supplier: Rockland Immunochemicals
Description: Running Buffer, TAE buffer solution 50X concentrate (TRIS-acetate-EDTA buffer)
UOM: 1 * 1 L
Image Unavailable-786-663

Blocking buffers, Protein-Free™

Supplier: G-Biosciences
Description: Protein-Free™ Blocking buffer is a formulation of non-protein agents that eliminate non-specific binding sites in ELISA, blotting, immunohistochemistry and other applications. The absence of protein from the Protein-Free™ Blocking buffer eliminates problems associated with traditional protein based blockers, such as cross-reactivity and interference from glycosylated proteins.

Image Unavailable-ROCKMB-045

SSC buffer, 20X concentrate, pH 7,0

Catalog Number: (ROCKMB-045)
Supplier: Rockland Immunochemicals
Description: Saline-sodium citrate (SSC) buffer is most commonly used as a buffer for the hybridisation process and washing in experiments where spcies of nucleic or ribonucleic acid are to be matched to complimentary sequences (such as Southern and Nouthern blotting, <i>in situ</i> hybridisation, or DNA microarrays). SSC is used to help control the stringency of hybridisation in these steps.
UOM: 1 * 1 L
Product Image-GENO786-701

Electrophoresis running buffers

Supplier: G-Biosciences
Description: There are many electrophoresis running buffers including: TAE running buffer (50×), TBE running buffer (10×), TBE-urea sample buffer, MOPS running buffer (10×), and MOPS/ SDS running buffer (20×). These include the SDS-PAGE sample loading buffer, SDS-PAGE running buffer, native sample loading buffer, tris glycine native gel running buffer, tricine sample buffer, tris-tricine, tris-tricine-SDS, MES SDS running buffer, MOPS/ SDS running buffer, IEF anode buffer, IEF cathode buffer, zymogram sample buffer, zymogram renature buffer, zymogram development buffer and the discontinuous buffer system.

Image Unavailable-ROCKMB-018

2X SDS-PAGE sample buffer without DTT or b-ME

Catalog Number: (ROCKMB-018)
Supplier: Rockland Immunochemicals
Description: Sample buffer for protein electrophoresis.
UOM: 1 * 100 mL
Product Image-B52

Buffer, TBE solution 10X concentrate (TRIS-borate-EDTA buffer)

Catalog Number: (B52)
Supplier: Thermo Fisher Scientific
Description: Running Buffer, TBE buffer solution 10X concentrate (TRIS-borate-EDTA buffer)
UOM: 1 * 1 KIT
Product Image-B49

Buffer, TAE solution 50X concentrate (TRIS-acetate-EDTA buffer)

Catalog Number: (B49)
Supplier: Thermo Fisher Scientific
Description: Running Buffer, TAE buffer solution 50X concentrate (TRIS-acetate-EDTA buffer)
UOM: 1 * 1 KIT

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Product Image-786-666

Blocking buffers, FirstChoice™

Supplier: G-Biosciences
Description: FirstChoice™ Blocking Buffer is ideal as a first choice for optimization of new assays and systems, or when determining the optimal blocking buffer for elimination of non specific binding sites in ELISA, blotting, immunohistochemistry and other applications. Buffers are conveniently supplied in widely used TBS (Tris-buffered saline at pH 7,5) and PBS (phosphate-buffered saline at pH 7,5) buffers as well as in separate formulations containing Tween® 20 for improving blocking efficiencies.

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Buffer, TBS solution 10X concentrate (TRIS buffered saline)

Supplier: G-Biosciences
Description: The TBS solution is primarily used as a wash and blocking buffer diluent for Western blot, ELISA and immunoassays.

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Product Image-A0965.9010

Buffer, PBS (10X, Dulbecco's) powder pack

Catalog Number: (A0965.9010)
Supplier: PanReac AppliChem
Description: Powder mixture to prepare PBS buffer (10X) according to Dulbecco.
UOM: 1 * 1 KIT

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Image Unavailable-ROCKMB-044

SSPE Buffer, 20X concentrate, pH 7,4

Catalog Number: (ROCKMB-044)
Supplier: Rockland Immunochemicals
Description: 20X SSPE buffer (3,0 M sodium chloride, 0,2 M sodium hydrogen phosphate, 0,02 M EDTA, pH 7,4) - DEPC treated.
UOM: 1 * 1 L
Image Unavailable-CAYM600220-250

Buffer, TBS solution 10X concentrate (TRIS buffered saline) with phenol red, pH 7,4

Supplier: Cayman Chemical
Description: 250 mM Tris-base, pH 7,4, 1,37 M sodium chloride, containing 27 mM KCl, 0,4 mM phenol red.

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on +353 1 88 22222.
Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on +353 1 88 22222
This product is marked as restricted and can only be purchased by approved Shipping Accounts. If you need further assistance, email VWR Regulatory Department at eurega_services@eu.vwr.com
-Additional Documentation May be needed to purchase this item. A VWR representative will contact you if needed.
This product has been blocked by your organisation. Please contact your purchasing department for more information.
The original product is no longer available. The replacement shown is available.
Product(s) marked with this symbol are discontinued - sold till end of stock. Alternatives may be available by searching with the VWR Catalog Number listed above. If you need further assistance, please call VWR Customer Service on +353 1 8822222.
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