2',7'-Dichlorofluorescein ~90% (by TLC), powder chemical indicator, Sigma-Aldrich®

Supplier: SIGMA-ALDRICH MICROSCOPY

Synonyms: 2',7'-Dichloro-3',6'-dihydroxy-3H-spiro[isobenzofuran-1,9'-xanthen]-3-one

M-Clarity™ quality level = MQ200

D6665-25G D6665-5G
D6665-25GEA 739 GBP
D6665-25G D6665-5G
2',7'-Dichlorofluorescein ~90% (by TLC), powder chemical indicator, Sigma-Aldrich®
2',7'-Dichlorofluorescein

2′,7′-Dichlorofluorescein is an oxidation-sensitive fluorescent probe. 2′7′-dichlorofluorescein diacetate is oxidized to 2′7′-dichlorofluorescein in the presence of ROS (reactive oxygen species). 2′,7′-Dichlorofluorescein has been used as an oxidation-sensitive fluorescent probe to measure ROS (reactive oxygen species) formation in cells.

Formula: C₂₀H₁₀Cl₂O₅
MW: 401,20 g/mol
Melting Pt: 280 °C (dec., lit.)
Density: 0,79 g/cm³
MDL Number: MFCD00005047
CAS Number: 76-54-0
EINECS: 200-968-6
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Specification Test Results

Appearance (form) Powder
Purity (TLC area %) ~90% (85 - 100%)
Solubility (colour) Very faint brown-yellow to very dark brown-yellow to very dark orange
Solubility (turbidity) Clear to hazy
Solubility (method) 25 mg/ml in ethanol
Application test Suitable for use as an indicator in chloride titrations
Recommended retest period 48 months

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