Alcian blue 8GX, powder, Sigma-Aldrich®
Supplier: SIGMA-ALDRICH MICROSCOPY
|
Warning
|
M-Clarity™ quality level = MQ100
Alcian Blue 8GX, also known as Ingrain Blue 1, is a basic copper phthalocyanine dye whose copper content imparts a blue color. It is tetravalent, cationic, and water-soluble in nature. It forms salt linkages with the acid groups of acid mucopolysaccharides.
- Alcian Blue 8GX has been used for the staining and precipitation of glycosaminoglycans and as a heteroglycan stain for neutral, sulfated, and phosphated mucopolysaccharides and glycosaminoglycans in tissues such as cartilage and extracellular matrices
- It has been employed in the in vivo visualization of intravascular Bonghan ducts and corpuscles in murine models
- It has been used to develop a novel histochemical staining method to assess multiple respiratory parameters in various species
- Desired staining patterns can be obtained by manipulating pH and salt concentrations
The Alcian blue staining is easily combined with the Periodic acid-Schiff (PAS) reaction. In the tissue, acid mucosubstances are stained selectively and nuclear fast red is commonly used as counterstain. In combination with the PAS reaction, non-substituted polysaccharides, neutral mucopolysaccharides, muco- and glycoproteins, and glycol- and phospholipids can be visualized in addition. An Acetic acid-Alcian blue solution is used for the staining, whereby carboxylated and sulfatic proteoglycans are selectively stained when a pH of 2.5 is applied. The differentiation between carboxyl and sulfate groups is only possible at pH 1.
Formula:
C₅₆H₆₈Cl₄CuN₁₆S₄ MW: 1298,86 g/mol Storage Temperature: Room Temperature |
MDL Number:
MFCD00010720 CAS Number: 33864-99-2 EINECS: 251-705-7 |
Specification Test Results
Appearance (Colour) | Dark Blue to Very Dark Blue |
Appearance (Form) | Powder |
Wavelength | 615 - 630 nm |
E1% 615-630 nm in H₂O |
≥190 |
Dye Content UV Spectroscopy, Based on E1% = 425 at 615-630 nm |
45 - 65 % |
Solubility (Colour) 1 mg/ml, H₂O | Dark Blue to Very Dark Blue and Dark Blue-Green to Very Dark Blue-Green |
Learn more
About VWR
Avantor is a vertically integrated, global supplier of discovery-to-delivery solutions for...