Naphthalene black 10B (naphthol blue black, acid black 1) 80% (dye content), Sigma-Aldrich®

Supplier: SIGMA-ALDRICH MICROSCOPY
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Synonyms: Sodium 4-amino-5-hydroxy-3-((4-nitrophenyl)diazenyl)-6-(phenyldiazenyl)naphthalene-2,7-disulphonate, Amido Black 10B

M-Clarity™ quality level = MQ200

195243-100G 195243-25G
195243-100GEA 173 GBP
195243-100G 195243-25G
Naphthalene black 10B (naphthol blue black, acid black 1) 80% (dye content), Sigma-Aldrich®
Naphthalene Black 10B (Naphthol Blue Black, Acid Black 1)

Naphthol blue black (NBB) is an textile diazo dye, with high photo- and thermal-stability. Photoelectrochemical degradation of this dye yields colorless quinone as end product. Pure grade of NBB is generally used for dyeing foodstuffs, drugs and cosmetics. It is also used as a protein stain in chromatography and electrophoresis and as a biological stain. Commercial grade of NBB is a widely used textile dye. It is used in dyeing wool, nylon, silk and in textile printing. In addition, it is also employed for colouring of soaps, anodised aluminum and casein, wood stains and writing ink preparation.


  • Naphthol blue black (NBB) has been used to stain soluble proteins

Formula: C₂₂H₁₄N₆Na₂O₉S₂
MW: 616,49 g/mol
Storage Temperature: Ambient
MDL Number: MFCD00004017
CAS Number: 1064-48-8
EINECS: 213-903-1
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Specification Test Results

Appearance (Color) Brown to Very Dark Brown and Black
Appearance (Form) Powder
Infrared spectrum Conforms to Structure
Wavelength c = 0.007g/l in H2O 616 - 622 nm
Extinction Coefficient ≥20000
Wavelength 318 - 324 nm
TLC Consistent With Control Conforms
Solubility (Turbidity) Opaque (c = 1mg/ml; H2O) Conforms
Solubility (Color) Dark Blue to Very Dark Blue
Water (by Karl Fischer) ≤10.0 %
Carbon ≥33.4 %
Nitrogen ≥10.6 %

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