Methyl green zinc chloride salt ~85%, Sigma-Aldrich®

Supplier: SIGMA-ALDRICH MICROSCOPY
Danger

Synonyms: CI 42590Methyl green zinc chloride double saltMethyl GreenEthyl Green

M-Clarity™ quality level = MQ200

M8884-5GEA 51.8 GBP
M8884-5G M8884-25G
Methyl green zinc chloride salt ~85%, Sigma-Aldrich®
Methyl Green zinc chloride salt

Methyl green is a synthetic, triphenylmethane-type cationic dye related to ethyl green mainly used as DNA stain in histochemistry. It possesses aniline rings with different degrees of methylation. The dye binds specifically to the A-T rich regions of the major groove of DNA with the help of two positive charges. Methyl green in combination with pyronin is extensively used to stain RNA and plasma cells in tissue sections and cytologic preparations.


Methyl green is suitable as a nuclear counterstain in enzyme histochemistry, immunostaining, and in situ hybridisation as the bluish-green color will be in contrast with red, purple, brown, and black products of histochemical reactions. It is also suitable as a tracking dye in Acid-polyacrylamide gel electrophoresis to separate gliadins. It has been used in the histochemical staining for the apoptosis examination of kidney tissue and as a nuclear stain for labeling embryos. Material may be a mixture of monobromo-monochloro and dichloro ZnCl₂ salts. Halogen contents listed on label.

Formula: C₂₇H₃₅BrClN₃·xZnCl₂
MW: 653,24 g/mol
Melting Pt: >300 °C (lit.)
Storage Temperature: Room Temperature
MDL Number: MFCD00151094
CAS Number: 7114-03-6
EINECS: 230-415-4

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Specification Test Results

Appearance (Colour) Red to Very Dark Red and Red-Brown and Brown-Red
Appearance (Form) Powder
Infrared spectrum Conforms to Structure
Wave Length, 0,005 G/L H₂O 626 - 634 nm
Extinction Coefficient ≥60000
Wave Length 417 - 425 nm
Extinction Coefficient ≥11100
Wave Length 311 - 319 nm
Extinction Coefficient ≥16000
Solubility (Colour), C = 1 mg/ml, H₂O Dark Blue to Very Dark Blue and Dark Blue-Green to Very Dark Blue-Green
Dye Content ≥83 %

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