Azure A ≥70% (dye content), Sigma-Aldrich®
Supplier: SIGMA-ALDRICH MICROSCOPY
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Warning
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Synonyms:
N,N-Dimethylthionine, 3-Amino-7-(dimethylamino)phenothiazin-5-ium chloride, Methylene Azur A
M-Clarity™ quality level = MQ200
Azure A is a basic cationic dye. The combination of azure A and silver staining is used for the quantification of glycosaminoglycans in agarose gels. It is also used for the staining of chromosomes in the nucleus. It is a main component of the azure B/polychromed methylene blue – eosin stains family, also referred to as the Romanowsky stain.
Azure A chloride has been used for the staining of polyacrylamide gel to analyse the bovine lung heparin digestion by heparinase I. It has been used for the determination of the sulphated groups content in polysaccharides.
Caution: Handle and store under argon. Glove bag item.
Formula:
C₁₄H₁₄ClN₃S MW: 291,797 g/mol Melting Pt: 290 °C (dec., lit.) Storage Temperature: Room Temperature |
MDL Number:
MFCD00012112 CAS Number: 531-53-3 EINECS: 208-510-7 |
Specification Test Results
Appearance (Colour) | Blue to Very Dark Blue to Very Dark Green |
Appearance (Form) | Powder or Crystals |
Infrared spectrum | Conforms to Structure |
Solubility (Colour) at 4 mg Plus 4 ml of Water | Dark Blue to Very Dark Blue and Dark Purple to Very Dark Purple and Blue-Purple |
Nitrogen | 10.0 - 14.4 % |
Dye Content | ≥70 % |
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