Azure A ≥70% (dye content), Sigma-Aldrich®

Supplier: SIGMA-ALDRICH MICROSCOPY
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Synonyms: N,N-Dimethylthionine, 3-Amino-7-(dimethylamino)phenothiazin-5-ium chloride, Methylene Azur A

M-Clarity™ quality level = MQ200

A6270-10G A6270-50G
A6270-10GEA 60.9 GBP
A6270-10G A6270-50G
Azure A ≥70% (dye content), Sigma-Aldrich®
Azure A

Azure A is a basic cationic dye. The combination of azure A and silver staining is used for the quantification of glycosaminoglycans in agarose gels. It is also used for the staining of chromosomes in the nucleus. It is a main component of the azure B/polychromed methylene blue – eosin stains family, also referred to as the Romanowsky stain.


Azure A chloride has been used for the staining of polyacrylamide gel to analyse the bovine lung heparin digestion by heparinase I. It has been used for the determination of the sulphated groups content in polysaccharides.


Caution: Handle and store under argon. Glove bag item.

Formula: C₁₄H₁₄ClN₃S
MW: 291,797 g/mol
Melting Pt: 290 °C (dec., lit.)
Storage Temperature: Room Temperature
MDL Number: MFCD00012112
CAS Number: 531-53-3
EINECS: 208-510-7
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Specification Test Results

Appearance (Colour) Blue to Very Dark Blue to Very Dark Green
Appearance (Form) Powder or Crystals
Infrared spectrum Conforms to Structure
Solubility (Colour) at 4 mg Plus 4 ml of Water Dark Blue to Very Dark Blue and Dark Purple to Very Dark Purple and Blue-Purple
Nitrogen 10.0 - 14.4 %
Dye Content ≥70 %

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