Azure A 70% (dye content) certified by the Biological Stain Commission, Sigma-Aldrich®
Supplier: SIGMA-ALDRICH MICROSCOPY
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Warning
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Synonyms:
N,N-Dimethylthionine, 3-Amino-7-(dimethylamino)phenothiazin-5-ium chloride, Methylene Azur A
M-Clarity™ quality level = MQ200
The combination of azure A and silver staining is used for the quantification of glycosaminoglycans in agarose gels. It is also used for the staining of chromosomes in the nucleus. Azure A is a main component of the azure B/polychromed methylene blue-eosin stains family, also referred to as the Romanowsky stain. Azure A is a basic cationic dye.
Azure A has been used to determine the amount of charged dextran sulfate in the nanoparticles for the study involving incorporation of heparin-binding proteins into preformed dextran sulfate-chitosan nanoparticles. Certified for use in Lillie′s modified Nocht′s method and the NCCLS method for blood smears.
Formula:
C₁₄H₁₄ClN₃S MW: 291,797 g/mol Melting Pt: 290 °C (dec., lit.) Storage Temperature: Room Temperature |
MDL Number:
MFCD00012112 CAS Number: 531-53-3 EINECS: 208-510-7 |
Specification Test Results
Appearance (Colour) | Blue to Very Dark Blue to Very Dark Green |
Appearance (Form) | Powder or Crystals |
Infrared spectrum | Conforms to Structure |
Wave Length, c = 0.003g/L; Water | 620 - 634 nm |
Extinction Coefficient | ≥11000 |
Wave Length | 242 - 246 nm |
Solubility (Turbidity), c = 1 mg/ml; Water | Turbid |
Solubility (Colour) | Dark Blue to Very Dark Blue and Dark Purple to Very Dark Purple and Blue-Purple |
Biological Stain Commision, Commission Certified Number: | Pass |
Dye Content | Approximately 70% |
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