Azure A 70% (dye content) certified by the Biological Stain Commission, Sigma-Aldrich®

Supplier: SIGMA-ALDRICH MICROSCOPY
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Synonyms: N,N-Dimethylthionine, 3-Amino-7-(dimethylamino)phenothiazin-5-ium chloride, Methylene Azur A

M-Clarity™ quality level = MQ200

861049-25G 861049-5G
861049-25GEA 321 GBP
861049-25G 861049-5G
Azure A 70% (dye content) certified by the Biological Stain Commission, Sigma-Aldrich®
Azure A

The combination of azure A and silver staining is used for the quantification of glycosaminoglycans in agarose gels. It is also used for the staining of chromosomes in the nucleus. Azure A is a main component of the azure B/polychromed methylene blue-eosin stains family, also referred to as the Romanowsky stain. Azure A is a basic cationic dye.


Azure A has been used to determine the amount of charged dextran sulfate in the nanoparticles for the study involving incorporation of heparin-binding proteins into preformed dextran sulfate-chitosan nanoparticles. Certified for use in Lillie′s modified Nocht′s method and the NCCLS method for blood smears.

Formula: C₁₄H₁₄ClN₃S
MW: 291,797 g/mol
Melting Pt: 290 °C (dec., lit.)
Storage Temperature: Room Temperature
MDL Number: MFCD00012112
CAS Number: 531-53-3
EINECS: 208-510-7
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Specification Test Results

Appearance (Colour) Blue to Very Dark Blue to Very Dark Green
Appearance (Form) Powder or Crystals
Infrared spectrum Conforms to Structure
Wave Length, c = 0.003g/L; Water 620 - 634 nm
Extinction Coefficient ≥11000
Wave Length 242 - 246 nm
Solubility (Turbidity), c = 1 mg/ml; Water Turbid
Solubility (Colour) Dark Blue to Very Dark Blue and Dark Purple to Very Dark Purple and Blue-Purple
Biological Stain Commision, Commission Certified Number: Pass
Dye Content Approximately 70%

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