5-Fluoroorotic acid enzyme substrate

Supplier: Thermo Fisher Scientific

Synonyms: 5-FOA, 2,6-Dihydroxy-5-fluoropyrimidine-4-carboxylic acid, 5-Fluoro-2,6-dioxo-1,2,3,6-tetrahydropyrimidine-4-carboxylic acid, 5-Fluorouracil-4-carboxylic acid

R0811 R0812
R0811EA 104 GBP
R0811 R0812
5-Fluoroorotic acid enzyme substrate
5-Fluoroorotic acid

Thermo Scientific 5-Fluoroorotic Acid (5-fluorouracil-6-carboxylic acid monohydrate; 5-FOA) is used in yeast molecular genetics to detect expression of the URA3 gene, which encodes orotine-5'-monophosphate (OMP) dicarboxylase


Yeast strains carrying a mutation in the URA3 gene grow in the presence of 5-FOA if the media is supplemented with uracil. 5-FOA is frequently used in experiments with Saccharomyces cerevisiae (URA3), Schizosaccharomyces pombe (URA4 and URA5), Candida albicans (URA3), and E. coli (pyrF).


Yeasts with an active URA3 gene (Ura+) convert 5-FOA to fluorodeoxyuridine, which is toxic to cells

Formula: C₅H₃FN₂O₄
MW: 174,09 g/mol
MDL Number: MFCD00042526
CAS Number: 703-95-7
EINECS: 211-876-0

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