The simple, modular design of miniVE vertical electrophoresis system consolidates all the small parts for electrophoresis and blotting into one convenient unit. Uniform gel temperature is maintained with the tank-style lower buffer chamber. A full tank stabilises the temperature so that lanes are straight and bands are flat. The blot module will accept two gel/membrane stacks simultaneously. The lower buffer chamber is designed to hold two blot modules, allowing four gels to be blotted at once.
- Perform 1-D and 2-D electrophoresis and electrotransfer in a single compact unit
- Cast, run, and blot mini-gels in four streamlined components: gel module, blot module, lower buffer chamber, safety lid
- Run two mini-gels or four mini-blots at once, using 10×10,5 cm gel cassettes for increased separation distances
- Complete semi-wet blotting in 45 minutes with only 300 ml buffer
- Choose precast gels from a variety of manufacturers
- Perform two second-dimension separations using 7 cm IPG strips in 90 minutes
The gel module is simultaneously a casting stand and an upper buffer chamber with integrated clamps. Simply assemble the gel sandwich of plates and spacers, clamp it onto the upper buffer chamber, then pivot the sealing gasket and cast the gel. After polymerisation, simply release the bottom seal and drop the assembly into the tank for running. No extra parts are needed and no unclamping until the run is finished.
The miniVE handles gel cassettes up to 10×10,5 cm (W×H), which increases the separation length by more than 30% over conventional 10×8 cm gels. In addition, miniVE is compatible with the glass plates, combs, and spacers of SE 260 mini-vertical units. For gradient gels, mini-vertical gel casters can produce up to four identical gels at one time.
EPS 301 is the power supply of choice for the miniVE System. With ratings of 300 V and 400 mA, it is capable of both running and blotting gels. For faster transfers when blotting four gels at a time, use the EPS 2A200 for its higher current capability.
Certifications: The product fulfills valid directives and standards when used within the conditions specified in the user manual. The product must also be used in the same state as it was delivered from Cytiva and connected only to other CE labeled Cytiva modules or other products as recommended. For regulatory details please see declaration of conformity.
Delivery information: Each complete system includes 3× rectangular glass plates, 3× notched plates, 2× gel modules, lid, lower buffer chamber, 2× 1,0 mm thick 10 well combs and 2× 1,0 mm thick spacer sets. Optional miniVE blot module includes 3× Dacron sponges (¼" thick) and 25 sheets of blotting paper.
